Expression of pyruvate carboxylase in cultured oligodendroglial, microglial and ependymal cells

Neurochemical Research

Volumn 34, Issue 3, 2009, Pages 480-489

  Murin, Radovan ^a   Cesar, Marija ^a   Kowtharapu, Bhavani S ^a   Verleysdonk, Stephan ^a   Hamprecht, Bernd ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

Cultured cells; Ependymocytes; Immunocytochemistry; Microglia; Oligodendroglia; Pyruvate carboxylase

Indexed keywords

ASPARAGINE; ASPARTIC ACID; GLUTAMIC ACID; GLUTAMINE; GLYCOGEN; NONESSENTIAL AMINO ACID; PYRUVATE CARBOXYLASE; REACTIVE OXYGEN METABOLITE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; ASTROCYTE; CARBOXYLATION; CELL METABOLISM; CITRIC ACID CYCLE; CONTROLLED STUDY; CORPUS CALLOSUM; ENZYME ACTIVITY; EPENDYMA CELL; GLIA CELL; GLUCONEOGENESIS; GLYCOGEN SYNTHESIS; HOMEOSTASIS; IMMUNOBLOTTING; IMMUNOCYTOCHEMISTRY; MICROGLIA; MYELINATION; NERVE CELL CULTURE; NERVE REGENERATION; NONHUMAN; OLIGODENDROGLIA; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PYRUVATE CARBOXYLASE DEFICIENCY; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSCRIPTION REGULATION;

ANIMALS; ANIMALS, NEWBORN; CELLS, CULTURED; EPENDYMA; IMMUNOHISTOCHEMISTRY; MICROGLIA; OLIGODENDROGLIA; PYRUVATE CARBOXYLASE; RATS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

ORYCTOLAGUS CUNICULUS;

EID: 59849113735     PISSN: 03643190     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11064-008-9806-6     Document Type: Article

References (55)

1. S Jitrapakdee JC Wallace 2003 The biotin enzyme family: conserved structural motifs and domain rearrangements Curr Protein Pept Sci 4 217 229 10.2174/1389203033487199
2. MF Utter DB Keech 1960 Formation of oxaloacetate from pyruvate and carbon dioxide J Biol Chem 235 PC17 PC18
3. OE Owen SC Kalhan RW Hanson 2002 The key role of anaplerosis and cataplerosis for citric acid cycle function J Biol Chem 277 30409 30412 10.1074/jbc.R200006200
4. S Jitrapakdee JC Wallace 1999 Structure, function and regulation of pyruvate carboxylase Biochem J 340 1 16 10.1042/0264-6021:3400001
5. 14C]lactate into the glycogen of cultured mouse astroglial cells. Evidence for gluconeogenesis in brain cells Biol Chem Hoppe Seyler 374 343 347
6. H Wiesinger B Hamprecht R Dringen 1997 Metabolic pathways for glucose in astrocytes Glia 21 22 34
7. FJ Ballard RW Hanson L Reshef 1970 Immunochemical studies with soluble and mitochondrial pyruvate carboxylase activities from rat tissues Biochem J 119 735 742
8. JC Mackall MD Lane 1977 Role of pyruvate carboxylase in fatty acid synthesis: alterations during preadipocyte differentiation Biochem Biophys Res Commun 79 720 725 10.1016/0006-291X(77)91171-8
9. AJ Cooper F Plum 1987 Biochemistry and physiology of brain ammonia Physiol Rev 67 440 519
10. C Zwingmann 2007 The anaplerotic flux and ammonia detoxification in hepatic encephalopathy Metab Brain Dis 22 235 249 10.1007/s11011-007-9069-y
11. FJ Ballard RW Hanson 1967 The citrate cleavage pathway and lipogenesis in rat adipose tissue: replenishment of oxaloacetate J Lipid Res 8 73 79
12. GH Sterling JJ O'Neill 1978 Citrate as the precursor of the acetyl moiety of acetylcholine J Neurochem 31 525 530 10.1111/j.1471-4159.1978.tb02668.x
13. RP Shank GS Bennett SO Freytag 1985 Pyruvate carboxylase: an astrocyte-specific enzyme implicated in the replenishment of amino acid neurotransmitter pools Brain Res 329 364 367 10.1016/0006-8993(85)90552-9
14. 13C]glucose metabolism J Neurochem 61 315 323 10.1111/j.1471-4159.1993.tb03570.x
15. B Robinson 2001
16. S Jitrapakdee GW Booker AI Cassady 1997 The rat pyruvate carboxylase gene structure. Alternate promoters generate multiple transcripts with the 5′-end heterogeneity J Biol Chem 272 20522 20530 10.1074/jbc.272.33.20522
17. S Jitrapakdee Q Gong MJ MacDonald 1998 Regulation of rat pyruvate carboxylase gene expression by alternate promoters during development, in genetically obese rats and in insulin-secreting cells. Multiple transcripts with 5′-end heterogeneity modulate translation J Biol Chem 273 34422 34428 10.1074/jbc.273.51.34422
18. AC Yu J Drejer L Hertz 1983 Pyruvate carboxylase activity in primary cultures of astrocytes and neurons J Neurochem 41 1484 1487 10.1111/j.1471-4159. 1983.tb00849.x
19. M Cesar B Hamprecht 1995 Immunocytochemical examination of neural rat and mouse primary cultures using monoclonal antibodies raised against pyruvate carboxylase J Neurochem 64 2312 2318
20. Y Xu G Öz KF LaNoue 2004 Whole-brain glutamate metabolism evaluated by steady-state kinetics using a double-isotope procedure: effects of gabapentin J Neurochem 90 1104 1116 10.1111/j.1471-4159.2004.02576.x
21. 2 fixation increases with brain activity J Neurosci 24 11273 11279 10.1523/JNEUROSCI.3564-04.2004
22. B Hassel 2007
23. R Dringen 2005 Oxidative and antioxidative potential of brain microglial cells Antioxid Redox Signal 7 1223 1233 10.1089/ars.2005.7.1223
24. AM Cataldo RD Broadwell 1986 Cytochemical identification of cerebral glycogen and glucose-6-phosphatase activity under normal and experimental conditions. II. Choroid plexus and ependymal epithelia, endothelia and pericytes J Neurocytol 15 511 524 10.1007/BF01611733
25. SI Miekka KC Ingham D Menache 1982 Rapid methods for isolation of human plasma fibronectin Thromb Res 27 1 14 10.1016/0049-3848(82)90272-9
26. B Hamprecht F Löffler 1985 Primary glial cultures as a model for studying hormone action Methods Enzymol 109 341 345 10.1016/0076-6879(85)09097-8
27. C Prothmann J Wellard J Berger 2001 Primary cultures as a model for studying ependymal functions: glycogen metabolism in ependymal cells Brain Res 920 74 83 10.1016/S0006-8993(01)03021-9
28. R Murín S Verleysdonk M Rapp 2006 Immunocytochemical localization of 3-methylcrotonyl-CoA carboxylase in cultured ependymal, microglial and oligodendroglial cells J Neurochem 97 1393 1402 10.1111/j.1471-4159.2006.03819.x
29. D Giulian TJ Baker 1986 Characterization of ameboid microglia isolated from developing mammalian brain J Neurosci 6 2163 2178
30. J Hirrlinger A Resch JM Gutterer 2002 Oligodendroglial cells in culture effectively dispose of exogenous hydrogen peroxide: comparison with cultured neurones, astroglial and microglial cells J Neurochem 82 635 644 10.1046/j.1471-4159.2002.00999.x
31. R Murín A Schaer BS Kowtharapu 2008 Expression of 3-hydroxyisobutyrate dehydrogenase in cultured neural cells J Neurochem 105 1176 1186 10.1111/j.1471-4159.2008.05298.x
32. A Chapman-Smith JE Cronan Jr 1999 Molecular biology of biotin attachment to proteins J Nutr 129 477S 484S
33. MG Bixel B Hamprecht 2000 Immunocytochemical localization of beta-methylcrotonyl-CoA carboxylase in astroglial cells and neurons in culture J Neurochem 74 1059 1067 10.1046/j.1471-4159.2000.0741059.x
34. SE Crisp JB Griffin BR White 2004 Biotin supply affects rates of cell proliferation, biotinylation of carboxylases and histones, and expression of the gene encoding the sodium-dependent multivitamin transporter in JAr choriocarcinoma cells Eur J Nutr 43 23 31 10.1007/s00394-004-0435-9
35. L Salganicoff RE Koeppe 1968 Subcellular distribution of pyruvate carboxylase, diphosphopyridine nucleotide and triphosphopyridine nucleotide isocitrate dehydrogenases, and malate enzyme in rat brain J Biol Chem 243 3416 3420
36. JR Mendell JN Whitaker 1978 Immunocytochemical localization studies of myelin basic protein J Cell Biol 76 502 511 10.1083/jcb.76.2.502
37. WJ Streit GW Kreutzberg 1987 Lectin binding by resting and reactive microglia J Neurocytol 16 249 260 10.1007/BF01795308
38. D Schmoll M Cesar E Führmann 1995 Colocalization of fructose-1, 6-bisphosphatase and glial fibrillary acidic protein in rat brain Brain Res 677 341 344 10.1016/0006-8993(95)00214-B
39. N Baumann D Pham-Dinh 2001 Biology of oligodendrocyte and myelin in the mammalian central nervous system Physiol Rev 81 871 927
40. J Edmond TA Higa RA Korsak 1998 Fatty acid transport and utilization for the developing brain J Neurochem 70 1227 1234
41. TB Patel JB Clark 1980 Lipogenesis in the brain of suckling rats Studies on the mechansim of mitochondrial-cytosolic carbon transfer Biochem J 188 163 168
42. W Cammer 1991 Immunostaining of carbamoylphosphate synthase II and fatty acid synthase in glial cells in rat, mouse, and hamster brains suggests roles for carbonic anhydrase in biosynthetic processes Neurosci Lett 129 247 250 10.1016/0304-3940(91)90472-6
43. GM Kurz H Wiesinger B Hamprecht 1993 Purification of cytosolic malic enzyme from bovine brain, generation of monoclonal antibodies, and immunocytochemical localization of the enzyme in glial cells of neural primary cultures J Neurochem 60 1467 1474 10.1111/j.1471-4159.1993.tb03309.x
44. NA Elshourbagy JC Near PJ Kmetz 1990 Rat ATP citrate-lyase. Molecular cloning and sequence analysis of a full-length cDNA and mRNA abundance as a function of diet, organ, and age J Biol Chem 265 1430 1435
45. F Mochel P DeLonlay G Touati 2005 Pyruvate carboxylase deficiency: clinical and biochemical response to anaplerotic diet therapy Mol Genet Metab 84 305 312 10.1016/j.ymgme.2004.09.007
46. A Bal-Klara 1989 Effects of some antidepressant drugs on the activity of glial cell enzymes in culture Eur J Pharmacol 161 231 235 10.1016/0014-2999(89) 90850-9
47. T Suormala UN Wiesmann F Cruz 2002 Biotin-dependent carboxylase activities in different CNS and skin-derived cells, and their sensitivity to biotin-depletion Int J Vitam Nutr Res 72 278 286 10.1024/0300-9831.72.4.278
48. KE Miller BA Richards RM Kriebel 2002 Glutamine-, glutamine synthetase-, glutamate dehydrogenase- and pyruvate carboxylase-immunoreactivities in the rat dorsal root ganglion and peripheral nerve Brain Res 945 202 211 10.1016/S0006-8993(02)02802-0
49. LC Park H Zhang KF Sheu 1999 Metabolic impairment induces oxidative stress, compromises inflammatory responses, and inactivates a key mitochondrial enzyme in microglia J Neurochem 72 1948 1958 10.1046/j.1471-4159.1999.0721948.x
50. J Hirrlinger JM Gutterer L Kussmaul 2000 Microglial cells in culture express a prominent glutathione system for the defense against reactive oxygen species Dev Neurosci 22 384 392 10.1159/000017464
51. R Dringen 2000 Metabolism and functions of glutathione in brain Prog Neurobiol 62 649 671 10.1016/S0301-0082(99)00060-X
52. BS Winkler N DeSantis F Solomon 1986 Multiple NADPH-producing pathways control glutathione (GSH) content in retina Exp Eye Res 43 829 847 10.1016/S0014-4835(86)80013-6
53. R Dringen HH Hoepken T Minich 2007
54. Z Zhang T Weinschenk HJ Schluesener 2005 Uptake, intracellular distribution, and novel binding proteins of immunostimulatory CpG oligodeoxynucleotides in microglial cells J Neuroimmunol 160 32 40 10.1016/j.jneuroim.2004.10.030
55. S Verleysdonk W Hirschner J Wellard 2004 Regulation by insulin and insulin-like growth factor of 2-deoxyglucose uptake in primary ependymal cell cultures Neurochem Res 29 127 134 10.1023/B:NERE.0000010441.08234.ca