Technical modification of the balb/c 3T3 cell transformation assay: The use of serum-reduced medium to optimise the practicability of the protocol

Alternatives to Laboratory Animals

Volumn 36, Issue 6, 2008, Pages 653-665

  Hayashi, Kumiko ^a   Sasaki, Kiyoshi ^a   Asada, Shin ^a   Tsuchiya, Toshiyuki ^b   Hayashi, Makoto ^c   Yoshimura, Isao ^d   Tanaka, Noriho ^a   Umeda, Makoto ^a  

^a HATANO RESEARCH INSTITUTE   (Japan)

^b BANYU PHARMACEUTICAL CO LTD   (Japan)

^c NATIONAL INSTITUTE OF HEALTH SCIENCES   (Japan)

^d TOKYO UNIVERSITY OF SCIENCE   (Japan)

Author keywords

Balb c 3T3 cells; Cell transformation assay; Optimised protocol; Serum reduced medium; Two stage method

Indexed keywords

3 METHYLCHOLANTHRENE; CADMIUM CHLORIDE; ETHANOLAMINE; INSULIN; LITHOCHOLIC ACID; MESYLIC ACID METHYL ESTER; METHYLNITRONITROSOGUANIDINE; MITOMYCIN C; PHENACETIN; PHENOBARBITAL; PHORBOL 13 ACETATE 12 MYRISTATE; SODIUM SELENITE; TRANSFERRIN;

ANIMAL CELL; ARTICLE; BAGG ALBINO MOUSE; BLOOD LEVEL; CELL ASSAY; CELL CLONE; CELL CULTURE; CELL STRAIN 3T3; CELL TRANSFORMATION; CONTROLLED STUDY; COST; CULTURE MEDIUM; GROWTH CURVE; MOUSE; NONHUMAN; PRIORITY JOURNAL; SERUM; TUMOR;

BOVINAE;

EID: 59849107330     PISSN: 02611929     EISSN: None     Source Type: Journal    
DOI: 10.1177/026119290803600609     Document Type: Article

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