Improvement of carcinogen detection in the BALB/3T3 cell transformation assay by using a rich basal medium supplemented with low concentration of serum and some growth factors

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 393, Issue 1-2, 1997, Pages 81-90

  Kajiwara, Yoshitsugu ^a   Ajimi, Syozo ^a   Hosokawa, Asami ^b   Maekawa, Kazuyuki ^a  

^a Chemical Biotesting Center   (Japan)

^b HACHINOHE NATIONAL COLLEGE OF TECHNOLOGY   (Japan)

Author keywords

BALB 3T3 cell; Improvement of cell transformation assay; Low serum concentration

Indexed keywords

CARCINOGEN; DIMETHYL SULFOXIDE; ETHANOLAMINE; GROWTH FACTOR; INSULIN; MESYLIC ACID METHYL ESTER; METHYLNITRONITROSOGUANIDINE; METHYLNITROSOUREA; PHORBOL 13 ACETATE 12 MYRISTATE; QUINOLINOL DERIVATIVE; SODIUM SELENITE; TRANSFERRIN;

ANIMAL CELL; ARTICLE; CELL STRAIN 3T3; CELL TRANSFORMATION; CONTROLLED STUDY; CULTURE MEDIUM; FETAL CALF SERUM; GENOTOXICITY; MOUSE; NONHUMAN; PRIORITY JOURNAL; SERUM;

3T3 CELLS; ANIMALS; CARCINOGENICITY TESTS; CARCINOGENS; CATTLE; CELL TRANSFORMATION, NEOPLASTIC; CULTURE MEDIA; EVALUATION STUDIES; GROWTH SUBSTANCES; MICE; MUTAGENS; PREDICTIVE VALUE OF TESTS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

ANIMALIA; CICONIIFORMES; LONCHOCARPUS GLAUCIFOLIUS;

EID: 0030722301     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1383-5718(97)00088-0     Document Type: Article

References (28)

1. McCann J., Spingarn N.E., Kobori J., Ames B.N. Detection of carcinogens as mutagens: bacterial tester strains with R factor plasmids. Proc. Natl. Acad. Sci. USA. 72:1975;979-983.
2. H.J. Evans, Cytological methods of detecting chemical mutagens, in: A. Hollaender (Ed.), Chemical Mutagens, Principles and Methods for Their Detection, vol. 4, Plenum Press, New York, 1976, pp. 1-25.
3. Kakunaga T. A quantitative system for assay of malignant transformation by chemical carcinogens using a clone derived from BALB/3T3. Int. J. Cancer. 12:1973;463-473.
4. Heidelberger C., Freeman A.E., Pienta R.J., Sivak A., Bertram J.S., Casto B.C., Dunkel V.C., Francis M.W., Kakunaga T., Little J.B., Schechtman L.M. Cell transformation by chemical agents - a review and analysis of the literature: A report of the United States Environmental Protection Agency. Mutation Res. 114:1983;283-385.
5. Smets L.A. Cell transformation as a model for tumor induction and neoplastic growth. Biochim. Biophys. Acta. 605:1980;93-111.
6. Meyer A.L. In vitro transformation assay for chemical carcinogens. Mutation Res. 115:1983;323-338.
7. Dunkel V.C., Pienta R.J., Sivak A., Traul K.A. Comparative neoplastic transformation responses of BALB/3T3 cells, Syrian hamster embryo cells, and Rauscher murine leukemia virus-infected Fischer 344 rat embryo cells to chemical carcinogens. J. Natl. Cancer Inst. 67:1981;1303-1315.
8. IARC/NCI/EPA Working Group Cellular and molecular mechanisms of cell transformation and standardization of transformation assay of established cell lines for the prediction of carcinogenic chemicals: Overview and recommended protocols. Cancer Res. 45:1985;2395-2399.
9. IARC, Experimental protocols recommended by the working group, IARC Sci. Publ. 67 (1985) 207-212.
10. Sakai A., Sato M. Improvement of carcinogen identification in BALB/3T3 cell transformation by application of a 2-stage method. Mutation Res. 214:1989;285-296.
11. Schechtman L.M. BALB/3T3 cell transformation: protocols, problems and improvements. IARC Sci. Publ. 67:1985;165-184.
12. Tsuchiya T., Umeda M. Improvement in the efficiency of the in vitro transformation assay method using BALB/3T3 A 31-1-1 cells. Carcinogenesis. 16:1995;1887-1894.
13. Ishidate M. Jr., Odashima S. Chromosome test with 134 compounds on Chinese hamster cells in vitro. A screening for chemical carcinogens. Mutation Res. 48:1977;337-354.
14. OECD, OECD guidelines for the testing of chemicals, Proposal for updating guideline 473, In vitro mammalian chromosome aberration test, 1995.
15. Le Boeuf R.A., Kerckaert G.A., Poiley J.A., Raineri R. An interlaboratory comparison of enhanced morphological transformation of Syrian hamster embryo cells cultured under conditions of reduced bicarbonate concentration and pH. Mutation Res. 222:1989;205-218.
16. Zeiger E., Haseman J.K., Shelby M.D., Margolin B.H., Tennant R.W. Evaluation of four in vitro genetic toxicity tests for predicting rodent carcinogenicity: Confirmation of earlier results with 41 additional chemicals. Environ. Mol. Mutagen. 16(Suppl. 18):1990;1-14.
17. Umeda M., Tanaka K., Ono T. Effect of insulin on the transformation of BALB/3T3 cells by X-ray irradiation. Gann. 74:1983;864-869.
18. Bertram J.S. Effects of serum concentration on the expression of carcinogen-induced transformation in the C3H/10T 1/2 Cl 8 cells line. Cancer Res. 37:1977;514-523.
19. Matthews E.J., Spalding J.W., Tennant R.W. Transformation of BALB/C-3T3 cells: V. Transformation responses of 168 chemicals compared with mutagenicity in Salmonella and carcinogenicity in rodent bioassays. Environ. Health Perspect. 101(Suppl. 2):1993;347-482.
20. National Toxicology Program, Bioassay of phenol for possible carcinogenicity (CAS No. 108-95-2) (NCI-CG-TR-203: NTP No. 80-15), US Department of Health and Human Services, Research Triangle Park, NC, 1980.
21. Boutwell P.K., Rusch H.P., Booth B. Tumor production by phenol and related compounds. Proc. Am. Assoc. Cancer Res. 2:1956;96.
22. Boutwell P.K., Bosch D.K. The tumor-promoting action of phenol and related compounds for mouse skin. Cancer Res. 19:1959;413-424.
23. Whynder E.L., Hoffman D. A study of tobacco carcinogenesis. The role of acidic fractions as promoters. Cancer. 14:1961;1306-1315.
24. Van Durren B.L., Goldschmit B.M. Cocarcinogenic and tumor-promoting agents in tobacco carcinogenesis. J. Natl. Cancer Inst. 56:1976;1237-1242.
25. Ames B.N., Mccann J., Yamasaki E. Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test. Mutation Res. 31:1975;347-364.
26. Tennant R.W., Zeiger E. Genetic toxicology: Current status of methods of carcinogen identification. Environ. Health Perspect. 100:1993;307-315.
27. Mirsalis J.C. In vivo measurement of unscheduled DNA synthesis and S-phase synthesis as an indicator of hepato-carcinogenesis in rodents. Cell Biol. Toxicol. 3:1987;165-173.
28. Mirsalis J.C., Tyson C.K., Steenmets K.L., Loh E.K., Hamilton C.M., Bakke J.P., Spalding J.W. Measurement of unscheduled DNA synthesis and S-phase synthesis in rodent hepatocytes following in vivo treatment: testing of 24 compounds. Environ. Mol. Mutagen. 14:1989;155-164.