Development of a quantitative real-time PCR assay for detection of Vibrio tubiashii targeting the metalloprotease gene

Journal of Microbiological Methods

Volumn 76, Issue 3, 2009, Pages 262-268

  Gharaibeh, Dima N ^a   Hasegawa, Hiroaki ^a   Häse, Claudia C ^a  

^a OREGON STATE UNIVERSITY   (United States)

Author keywords

Hatchery; Pacific oyster (Crassostrea gigas); Seawater; Vibriosis

Indexed keywords

BACTERIAL DNA; METALLOPROTEINASE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BACTERIUM DETECTION; GENE TARGETING; HATCHERY; NONHUMAN; NUCLEOTIDE SEQUENCE; OYSTER; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; VIBRIO; VIBRIO TUBIASHII;

ANIMALS; BASE SEQUENCE; COLONY COUNT, MICROBIAL; CRASSOSTREA; DNA PRIMERS; DNA, BACTERIAL; GENES, BACTERIAL; METALLOPROTEASES; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SEAWATER; SEQUENCE ANALYSIS, DNA; VIBRIO;

BACTERIA (MICROORGANISMS); BIVALVIA; CRASSOSTREA GIGAS; OSTREIDAE; VIBRIO; VIBRIO TUBIASHII;

EID: 59849083913     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2008.12.001     Document Type: Article

References (21)

1. Amaro C., Biosca E.G., Esteve C., Fouz B., and Toranzo A.E. Comparative study of phenotypic and virulence properties in V. vulnificus biotype 1 and 2 obtained from a European eel farm experiencing mortalities. Dis. Aquat. Org. 13 (1992) 29-35
2. Blackstone G.M., Nordstrom J.L., Bowen M.D., Meyer R.F., Imbro P., and DePaola A. Use of a real time PCR assay for detection of the ctxA gene of Vibrio cholerae in an environmental survey of Mobile Bay. J. Microbiol. Methods 68 (2007) 254-259
3. Davis B.R., Fanning R.G., Madden J.M., Steigerwalt A.G., Bradford H.B., Smith H.L., and Brenner D.J. Characterization of biochemically atypical Vibrio cholerae strains and designation of a new pathogenic species, Vibrio mimicus. J. Clin. Microbiol. 14 (1981) 631-639
4. Eiler A., Johansson M., and Bertilsson S. Environmental influences on Vibrio populations in Northern temperate and boreal coastal waters (Baltic and Skagerrak Seas). Appl. Environ. Microbiol. 72 (2006) 6004-6011
5. Elston R.A., Hasegawa H., Humphrey K.L., Polyak I.K., and Häse C.C. Re-emergence of Vibrio tubiashii in bivalve shellfish aquaculture: severity, environmental drivers, geographical extent and management. Dis. Aquat. Org. 82 (2008) 119-134
6. Estes R.M., Friedman C.S., Elston R.A., and Herwig R.P. Pathogenicity testing of shellfish hatchery bacterial isolates on Pacific oyster Crassostrea gigas larvae. Dis. Aquat. Org. 58 (2004) 223-230
7. Gordon K.V., Vickery M.C., DePaola A., Staley C., and Harwood V. Real-time PCR assays for quantification and differentiation of Vibrio vulnificus strains in oysters and water. Appl. Environ. Microbiol. 74 (2007) 1704-1709
8. Hada H.S., West P.A., Lee J.V., Stemmler J., and Colwell R.R. Vibrio tubiashii sp. nov., a pathogen of bivalve mollusks. Int. J. Syst. Bacteriol. 34 (1984) 1-4
9. Hasegawa H., Lind E.J., Boin M.A., and Häse C.C. The extracellular metalloprotease of Vibrio tubiashii is a major virulence factor for Pacific oyster (Crassostrea gigas) larvae. Appl. Environ. Microbiol. 13 (2008) 4101-4110
10. Hasegawa, H., Gharaibeh, D.N., Lind, E.J., Häse, C.C., submitted for publication. Virulence of metalloprotease produced by Vibrio species on Pacific oyster (Crassostrea gigas) larvae.
11. Hickman-Brenner F.W., Brenner D.J., Steigerwalt A.G., Schreiber M., Holmberg S.D., Baldy L.M., Lewis C.S., Pickens N.M., and Farmer 3rd J.J. Vibrio fluvialis and Vibrio furnissii isolated from a stool sample of one patient. J. Clin. Microbiol. 20 (1984) 125-127
12. Ririe K.M., Rasmussen R.P., and Wittwer C.T. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal. Biochem. 245 (1997) 154-160
13. Robert A., Silva A., Benitez J.A., Rodriguez B.L., Fando R., Campos J., Sengupta D.K., Boesman-Finkelstein M., and Finkelstein R.A. Tagging a Vibrio cholerae El Tor candidate vaccine strain by disruption of its hemagglutinin/protease gene using a novel reporter enzyme: Clostridium thermocellum endoglucanasea. Vaccine 14 (1996) 1517-1522
14. Le Roux F., Binesse J., Saulnier D., and Mazel D. Construction of a Vibrio splendidus mutant lacking the metalloprotease gene vsm by use of a novel counterselectable suicide vector. Appl. Environ. Microbiol. 73 (2007) 777-784
15. Sakazaki R. Proposal of Vibrio alginolyticus for biotype 2 Vibrio parahaemolyticus. Jpn. J. Med. Sci. Biol. 21 (1968) 326-359
16. Sindermann C.J. Vibriosis of larval oysters. In: Sindermann C.J., and Lightner D.V. (Eds). Disease Diagnosis and Control in North American Aquaculture (1988), Elsevier, Amsterdam 271-274
17. Takahashi H., Hara-Kudo Y., Miyasaka J., Kumagai S., and Konuma H. Development of a quantitative real-time polymerase chain reaction targeted to the toxR for detection of Vibrio vulnificus. J. Microbiol. Methods 61 (2004) 77-85
18. Thompson J.R., Randa M.A., Marcelino L.A., Tomita-Mitchell A., Lim E., and Polz M.F. Diversity and dynamics of a North Atlantic coastal Vibrio community. Appl. Environ. Microbiol. 70 (2004) 4103-4110
19. Tubiash H.S., Chanley P.E., and Leifson E. Bacillary necrosis, a disease of larval and juvenile bivalve mollusks. I. Etiology and epizootiology. J. Bacteriol. 90 (1965) 1036-1044
20. Wright A.C., Garrido V., Debuex G., Farrell-Evans M., Mudbidri A.A., and Otwell W.S. Evaluation of postharvest-processed oysters by using PCR-based most-probable-number enumeration of Vibrio vulnificus bacteria. Appl. Environ. Microbiol. 73 (2007) 7477-7481
21. Zhou S., Hou Z., Li N., and Qin Q. Development of a SYBR Green I real-time PCR for quantitative detection of Vibrio alginolyticus in seawater and seafood. J. Appl. Microbiol. 103 (2007) 1897-1906