Development and validation of HPLC method for imiquimod determination in skin penetration studies

Biomedical Chromatography

Volumn 22, Issue 12, 2008, Pages 1416-1423

  De Paula, Daniel ^a   Martins, Carolina Azenha ^a   Bentley, Maria Vitória Lopes Badra ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Franz diffusion cell; HPLC; Imiquimod; Skin; Tape stripping

Indexed keywords

FRANZ DIFFUSION CELLS; HPLC; HPLC METHOD; IMIQUIMOD; IN-VITRO; SIMPLE++; SKIN PENETRATION; SKIN SAMPLES; STRATUM CORNEUM; TAPE STRIPPING;

SKIN;

ACETIC ACID; DIETHYLAMINE; IMIQUIMOD; METHANOL; AMINOQUINOLINE DERIVATIVE; IMMUNOLOGICAL ADJUVANT;

ACCURACY; ANIMAL TISSUE; ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DERMIS; DIFFUSION; DRUG DETERMINATION; DRUG ISOLATION; DRUG PENETRATION; DRUG STRUCTURE; ELUTION; EPIDERMIS; FLOW RATE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IN VITRO STUDY; NONHUMAN; PH; QUANTITATIVE ANALYSIS; RADIATION DETECTION; SENSITIVITY ANALYSIS; SKIN PENETRATION; STRATUM CORNEUM; SWINE; ULTRAVIOLET RADIATION; ANIMAL; CHEMICAL STRUCTURE; CHEMISTRY; METABOLISM; METHODOLOGY; REPRODUCIBILITY; SKIN; SKIN ABSORPTION;

SUS;

ADJUVANTS, IMMUNOLOGIC; AMINOQUINOLINES; ANIMALS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; MOLECULAR STRUCTURE; REPRODUCIBILITY OF RESULTS; SKIN; SKIN ABSORPTION; SWINE;

EID: 59649116169     PISSN: 02693879     EISSN: 10990801     Source Type: Journal    
DOI: 10.1002/bmc.1075     Document Type: Article

References (26)

1. Bilu D and Sauder DN. Imiquimod: modes of action. British Journal of Dermatology 2003; 149(suppl. 66): 5-8.
2. Bronaugh RL and Maibach HI. Percutaneous Absorption: Drugs-Cosmetics-Mechanisms-Methodology. Marcel Dekker: New York, 1999.
3. Brown MB, Martin GP, Jones SA and Akomeah FK. Dermal and transdermal drug delivery systems: current and future prospects. Drug Delivery 2006; 13: 175-187.
4. Causon R. Validation of chromatographic methods in biomedical analysis. Viewpoint and discussion. Journal of Chromatography B: Biomedical Science Applications 1997; 689: 175-180.
5. Donnelly RF, McCarron PA, Zawislak AA and Woolfson AD. Design and physicochemical characterisation of a bioadhesive patch for dose-controlled topical delivery of imiquimod. International Journal of Pharmacy 2006; 307: 318-325.
6. Echevarria L, Blanco-Prieto MJ, Campanero MA, Santoyo S and Ygartua P. Development and validation of a liquid chromatographic method for in vitro mupirocin quantification in both skin layers and percutaneous penetration studies. Journal of Chromatography B 2003; 796: 233-341.
7. Elsayed MMA. Rapid determination of cinchocaine in skin by high-performance liquid chromatography. Biomedical Chromatography 2007; 21: 491-496.
8. Hadgraft J. Skin deep. European Journal of Pharmacy and Biopharmacy 2004; 58: 291-299.
9. Harrison LI, Skinner SL, Marbury TC, Owens ML, Kurup S, Mckane S and Greene RJ. Pharmacokinetics and safety of imiquimod 5% cream in the treatment of actinic keratosis of the face, scalp or hands and arms. Archives of Dermatology Research 2004; 296: 6-11.
10. Higuchi T and Connors KA. Phase solubility techniques. Advances in Analytical Chemistry Instrumentation 1965; 4: 177-212.
11. ICH. Validation of Analytical Procedures: Methodology. International Conference on Harmonisation Topic Q2B, 1996.
12. Lindemann U, Wilken K, Weigmann HJ, Schaefer H, Sterry W and Lademann J. Quantification of the horny layer using tape stripping and microscopic techniques. Journal of Biomedical Optics 2003; 8: 601-607.
13. Lopes LB, Ferreira DA, de Paula D, Garcia MT, Thomazini JA, Fantini MC and Bentley MV. Reverse hexagonal phase nanodispersion of monoolein and oleic acid for topical delivery of peptides: in vitro and in vivo skin penetration of cyclosporin A. Pharmaceutical Research 2006a; 23: 1332-1342.
14. Lopes LB, Lopes JL, Oliveira DC, Thomazini JA, Garcia MT, Fantini MC, Collett JH and Bentley MV. Liquid crystalline phases of monoolein and water for topical delivery of cyclosporin A: characterization and study of in vitro and in vivo delivery. European Journal of Pharmacy and Biopharmacy 2006b; 63: 146-155.
15. Moser K, Kriwet K, Naik A, Kalia YN and Guy RH. Passive skin penetration enhancement and its quantification in vitro. European Journal of Pharmacy and Biopharmacy 2001; 52: 103-112.
16. Navi D and Huntley A. Imiquimod 5 percent cream and the treatment of cutaneous malignancy. Dermatology Online Journal 2004; 10: 4.
17. Nylander-French LA. A tape-stripping method for measuring dermal exposure to multifunctional acrylates. Annals of Occupational Hygiene 2000; 44: 645-651.
18. Ravichandran R, Osterheim JA, Tollefson M, Busse GM. UV Spectrometric Determination of Imiquimod in Topical Cream Formulations. 3M Drug Delivery Systems: St Paul, MN, 2003.
19. Schon MP and Schon M. Immune modulation and apoptosis induction: two: sides of the antitumoral activity of imiquimod. Apoptosis 2004; 9: 291-298.
20. Snyder LR, Kirkland JJ and Glajch JL. Practical HPLC Method Development. Wiley: Hoboken, NJ, 1997.
21. Soria I, Myhre P, Horton V, Ellefsun P, McCarville S, Schmitt K and Owens M. Effect of food on the pharmacokinetics and bioavailability of oral imiquimod relative to a subcutaneous dose. International Journal of Clinical Pharmacological Therapy 2000; 38: 476-481.
22. Stanley MA. Imiquimod and the imidazoquinolones: mechanism of action and therapeutic potential. Clinical and Experimental Dermatology 2002; 27: 571-577.
23. Steluti R, De Rosa FS, Collett J, Tedesco AC and Bentley MV. Topical glycerol monooleate/propylene glycol formulations enhance 5-aminolevulinic acid in vitro skin delivery and in vivo protophorphyrin IX accumulation in hairless mouse skin. European Journal of Pharmacy and Biopharmacu 2005; 60: 439-444.
24. Touitou E, Meidan VM and Horwitz E. Methods for quantitative determination of drug localized in the skin. Journal of Control Release 1998; 56: 7-21.
25. Turchiello RF, Vena FC, Maillard P, Souza CS, Bentley MV and Tedesco AC. Cubic phase gel as a drug delivery system for topical application of 5-ALA, its ester derivatives and m-THPC in photodynamic therapy (PDT). Journal of Photochemistry and Photobiology B 2003; 70: 1-6.
26. Wagstaff AJ and Perry CM. Topical imiquimod: a review of its use in the management of anogenital warts, actinic keratoses, basal cell carcinoma and other skin lesions. Drugs 2007; 67: 2187-2210.