Molecular cloning and recombinant expression of a gene encoding a fungal immunomodulatory protein from Ganoderma lucidum in Pichia pastoris

World Journal of Microbiology and Biotechnology

Volumn 25, Issue 3, 2009, Pages 383-390

  Lin, Jing Wei ^a   Hao, Li Xia ^a   Xu, Gui Xue ^a   Sun, Fei ^b   Gao, Feng ^c   Zhang, Ren ^d   Liu, Li Xia ^a  

^a NORTHEAST NORMAL UNIVERSITY   (China)

^b JILIN UNIVERSITY   (China)

^c DUKE UNIVERSITY   (United States)

^d UNIVERSITY OF WOLLONGONG   (Australia)

Author keywords

Fungal immunomodulatory protein; Ganoderma lucidum; Molecular cloning; Pichia pastoris; Recombinant expression

Indexed keywords

AMINES; AMINO ACIDS; ASSAYS; BIOACTIVITY; CELL DEATH; CLONING; DICHROISM; DIFFUSERS (OPTICAL); ENCODING (SYMBOLS); GENETIC ENGINEERING; ORGANIC ACIDS; TUMORS;

FUNGAL IMMUNOMODULATORY PROTEIN; GANODERMA LUCIDUM; MOLECULAR CLONING; PICHIA PASTORIS; RECOMBINANT EXPRESSION;

GENE ENCODING;

BASIDIOMYCOTA; GANODERMA LUCIDUM; PICHIA PASTORIS;

EID: 59449109408     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-008-9902-4     Document Type: Article

References (29)

1. HH Chang F Sheu 2006 Anti-tumor mechanisms of orally administered a fungal immunomodulatory protein from Flammulina velutipes in mice Nutr Immunol 20 A1057
2. S Chang JA Buswell 1999 Ganoderma lucidum-a mushrooming medical mushroom Int J Med Mushrooms 1 139 146
3. YH Chen JT Yang KH Chau 1974 Determination of the helix and β turn of proteins in aqueous solution by circular dichroism Biochemistry 13 3350 3359 10.1021/bi00713a027
4. D Cheng XY Huang BJ Li 2002 A method for preparation of high quality DNA from medical fungi and construction of a genomic library Mycosystema 21 137 139
5. JJ Clare FB Rayment SP Ballantine K Sreekrishna MA Romanos 1991 High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene Biotechnology 9 5 455 460 10.1038/nbt0591-455
6. M Haak-Frendscho K Kino K Sone P Jardieu 1993 Ling Zhi-8: a novel T cell mitogen induces cytokine production and upregulation of ICAM-1 expression Cell Immunol 150 101 113 10.1006/cimm.1993.1182
7. KY Hsieh CI Hsu JY Lin CC Tsai RH Lin 2003 Oral administration of an edible-mushroom-derived protein inhibits the development of food-allergic reactions in mice Clin Exp Allergy 33 1595 1602 10.1046/j.1365-2222.2003.01790.x
8. CW Hsieh JL Lan Q Meng YW Cheng HM Huang JJ Tsai 2007 Eosinophil apoptosis induced by fungal immunomodulatory peptide-fve via reducing IL-5 alpha receptor J Formos Med Assoc 106 1 36 43
9. HC Hsu CI Hsu RH Lin CL Kao JY Lin 1997 Fip-vvo, a new fungal immunomodulatory protein isolated from Vovariella volvacea Biochem J 323 557 565
10. HY Hsu KF Hua WC Wu J Hsu ST Weng TL Lin CY Liu RS Hseu CT Huang 2008 Reishi immuno-modulation protein induces interleukin-2 expression via protein kinase-dependent signaling pathways within human T cells J Cell Physiol 215 15 26 10.1002/jcp.21144
11. TR Jinn CM Wu WC Tu JL Ko TCT Jason 2006 Functional expression of FIP-gts, a fungal immunomodulatory protein from Ganoderma Tsugae in Sf21 insect cells Biosci Biotechnol Biochem 70 2627 2634 10.1271/bbb.60232
12. K Kino A Yamashita K Yamaoka J Watanabe S Tanaka K Ko K Shimizu H Tsunoo 1989 Isolation and characterization of a new immunomodulatory protein, Ling Zhi-8 (LZ-8), from Ganoderma lucidum J Biol Chem 264 472 478
13. K Kino K Mizumoto T Sone T Yamaji J Watanabe A Yamashita K Yamaoka K Shimizu K Ko H Tsunoo 1990 An immunomodulatory protein, Ling Zhi-8 (LZ-8) prevents insulitis in nonobese diabetic mice Diabetologia 33 713 718 10.1007/BF00400340
14. JL Ko CI Hsu RH Lin CL Jai JY Lin 1995 A new fungal immunomodulatory protein, FIP-fve isolated from the edible mushroom, Flammulina velutipes and its complete amino acid sequence Eur J Biochem 228 244 249 10.1111/j.1432-1033. 1995.tb20256.x
15. JL Ko SJ Lin CI Hsu CL Kao JY Lin 1997 Molecular cloning and expression of a fungal immunomodulatory protein, FIP-fve, from Flammulina velutipes J Formos Med Assoc 96 517 524
16. CH Liao YM Hsiao CP Hsu MY Lin JC Wang YL Huang JL Ko 2006 Transcriptionally mediated inhibition of telomerase of fungal immunomodulatory protein from Ganoderma tsugae in A549 human lung adenocarcinoma cell line Mol Carcinog 45 4 220 229
17. CH Liao YM Hsiao GT Sheu JT Chang PH Wang MF Wu GJ Shieh JL Ko 2007 Nuclear translocation of telomerase reverse transcriptase and calcium signaling in repression of telomerase activity in human lung cancer cells by fungal immunomodulatory protein from Ganoderma tsugae Biochem Pharmacol 74 1541 1554 10.1016/j.bcp.2007.07.025
18. WH Lin CH Huang CI Hsu JY Lin 1997 Dimerization of the N-terminal amphipathic α-helix domain of the fungal immunomodulatory protein from Ganoderma tsugae (Fip-gts) defined by a yeast two-hybrid system and site-directed mutagenesis J Biol Chem 272 20044 20048 10.1074/jbc.272.32.20044
19. YH Liu MC Kao YL Lai JJ Tsai 2003 Efficacy of local nasal immunotherapy for Dp2-induced airway inflammation in mice: using Dp2 peptide and fungal immunomodulatory peptide J Allergy Clin Immunol 112 301 310 10.1067/mai.2003. 1619
20. A Murasugi S Tanaka N Komiyama N Iwata K Kino H Tsunoo S Sakuma 1991 Molecular cloning of a cDNA and a gene encoding an immunomodulatory protein, Ling Zhi-8, from a fungus, Ganoderma lucidum J Biol Chem 266 2486 2493
21. P Paaventhan SJ Jeremiah VS See V Shai R Howard YC Kaw RK Prasanna 2003 A 1.7 Å structure of Fve, a member of the new fungal immunomodulatory protein family J Mol Biol 332 461 470 10.1016/S0022-2836(03)00923-9
22. SV Seow IC Kuo P Paaventhan PR Kolatkar KY Chua 2003 Crystallization and preliminary X-ray crystallographic studies on the fungal immunomodulatory protein Fve from the golden needle mushroom (Flammulina velutipes) Acta Crystallogr sect 59 1487 1489
23. DM Strong AA Ahmed GB Thurman KW Sell 1973 In vitro stimulation of murine spleen cells using a micro culture system and a multiple automated sample harvester J Immunol Methods 2 279 291 10.1016/0022-1759(73)90054-9
24. S Tanaka K Ko K Kino K Tsuchiya A Yamashita S Murasugi H Tsumoo 1989 Complete amino acid sequence of an immunomodulatory protein, Ling Zhi-8 J Biol Chem 264 16372 16377
25. LG Van der Hem JA van der Vliet CF Bocken K Kino AJ Hoitsma WJ Tax 1995 Ling Zhi-8: studies of a new immunomodulating agent Transplantation 60 5 438 443 10.1097/00007890-199509000-00006
26. PH Wang CI Hsu YL Tang JY Lin JL Ko 2004 Fungal immunomodulatory protein from Flammulina velutipes induces interferon-gamma production through p38 mitogen-activated protein kinase signaling pathway J Agric Food Chem 52 2721 2725 10.1021/jf034556s
27. AS Wermerskirchen DH LaTocha BL Clarle 2000 Adrenocorticotropic hormone controls concanavalin A activation of rat lymphocytes by modulating IL-2 production J Life Sci 67 2177 2187 10.1016/S0024-3205(00)00810-9
28. CM Wu TY Wu SS Kao JL Ko TR Jinn 2008 Expression and purification of a recombinant Fip-fve from Flammulina velutipes in baculovirus-infected insect cells J Appl Microbiol 104 1354 1362 10.1111/j.1365-2672.2007.03686.x
29. CM Yeh CK Yeh XY Hsu QM Luo MY Lin 2008 Extracellular expression of a functional recombinant Ganoderma lucidum immunomodulatory protein by Bacillus subtilis and Lactococcus lactis Appl Environ Microbiol 74 1039 1049 10.1128/AEM.01547-07