Chapter 7 The GST-BHMT Assay and Related Assays for Autophagy

Methods in Enzymology

Volumn 451, Issue C, 2009, Pages 97-118

  Dennis, Patrick B ^a   Mercer, Carol A ^a  

^a UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; BETAINE HOMOCYSTEINE METHYLTRANSFERASE; GLUTATHIONE TRANSFERASE; PROTEINASE INHIBITOR; HYBRID PROTEIN;

AUTOPHAGY; BIOCHEMISTRY; CELL CULTURE; CELL ORGANELLE; CELLULAR DISTRIBUTION; CYTOPLASM; CYTOSOL; ENDOPLASMIC RETICULUM; ENZYME ASSAY; ENZYME PURIFICATION; FRAGMENTATION REACTION; GENETIC TRANSFECTION; HUMAN; INTRACELLULAR SPACE; LYSOSOME; MEASUREMENT; MITOCHONDRION; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PURIFICATION; REVIEW; VALIDATION PROCESS; WESTERN BLOTTING; ARTICLE; BIOASSAY; CELL LINE; GENETICS; METABOLISM; METHODOLOGY; PHAGOSOME; PHYSIOLOGY;

MAMMALIA;

AUTOPHAGY; BETAINE-HOMOCYSTEINE S-METHYLTRANSFERASE; BIOLOGICAL ASSAY; CELL LINE; CYTOSOL; GLUTATHIONE TRANSFERASE; HUMANS; LYSOSOMES; PHAGOSOMES; RECOMBINANT FUSION PROTEINS;

EID: 59249109653     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(08)03607-0     Document Type: Review

References (29)

1. Chadwick L.H., McCandless S.E., Silverman G.L., Schwartz S., Westaway D., and Nadeau J.H. Betaine-homocysteine methyltransferase-2: cDNA cloning, gene sequence, physical mapping, and expression of the human and mouse genes. Genomics 70 (2000) 66-73
2. Chen C.A., and Okayama H. Calcium phosphate-mediated gene transfer: A highly efficient transfection system for stably transforming cells with plasmid DNA. Biotechniques 6 (1988) 632-638
3. Chen J.M., Dando P.M., Rawlings N.D., Brown M.A., Young N.E., Stevens R.A., Hewitt E., Watts C., and Barrett A.J. Cloning, isolation, and characterization of mammalian legumain, an asparaginyl endopeptidase. J. Biol. Chem. 272 (1997) 8090-8098
4. Chen J.M., Fortunato M., and Barrett A.J. Activation of human prolegumain by cleavage at a C-terminal asparagine residue. Biochem. J. 352 Pt 2 (2000) 327-334
5. Furuya N., Kanazawa T., Fujimura S., Ueno T., Kominami E., and Kadowaki M. Leupeptin-induced appearance of partial fragment of betaine homocysteine methyltransferase during autophagic maturation in rat hepatocytes. J. Biochem. (Tokyo) 129 (2001) 313-320
6. Hosokawa N., Hara Y., and Mizushima N. Generation of cell lines with tetracycline-regulated autophagy and a role for autophagy in controlling cell size. FEBS Lett. 580 (2006) 2623-2629
7. Kabeya Y., Mizushima N., Ueno T., Yamamoto A., Kirisako T., Noda T., Kominami E., Ohsumi Y., and Yoshimori T. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J. 19 (2000) 5720-5728
8. Klionsky D.J. The molecular machinery of autophagy: Unanswered questions. J. Cell Sci. 118 (2005) 7-18
9. Kopitz J., Kisen G.O., Gordon P.B., Bohley P., and Seglen P.O. Nonselective autophagy of cytosolic enzymes by isolated rat hepatocytes. J. Cell Biol. 111 (1990) 941-953
10. Kraft C., Deplazes A., Sohrmann M., and Peter M. Mature ribosomes are selectively degraded upon starvation by an autophagy pathway requiring the Ubp3p/Bre5p ubiquitin protease. Nat. Cell Biol. 10 (2008) 602-610
11. Lee D.H., and Goldberg A.L. Proteasome inhibitors: Valuable new tools for cell biologists. Trends Cell Biol. 8 (1998) 397-403
12. Lemasters J.J., Nieminen A.L., Qian T., Trost L.C., Elmore S.P., Nishimura Y., Crowe R.A., Cascio W.E., Bradham C.A., Brenner D.A., and Herman B. The mitochondrial permeability transition in cell death: A common mechanism in necrosis, apoptosis and autophagy. Biochim. Biophys. Acta 1366 (1998) 177-196
13. Li D.N., Matthews S.P., Antoniou A.N., Mazzeo D., and Watts C. Multistep autoactivation of asparaginyl endopeptidase in vitro and in vivo. J. Biol. Chem. 278 (2003) 38980-38990
14. Marshansky V., and Futai M. The V-type H(+)-ATPase in vesicular trafficking: Targeting, regulation and function. Curr. Opin. Cell Biol. 20 (2008) 415-426
15. Mercer C.A., Kaliappan A., and Dennis P.B. Macroautophagy-dependent, intralysosomal cleavage of a betaine homocysteine methyltransferase fusion protein requires stable multimerization. Autophagy 4 (2008) 185-194
16. Mizushima N., and Yoshimori T. How to Interpret LC3 Immunoblotting. Autophagy 3 (2007)
17. Neermann J., and Wagner R. Comparative analysis of glucose and glutamine metabolism in transformed mammalian cell lines, insect and primary liver cells. J. Cell Physiol. 166 (1996) 152-169
18. Noda T., Matsuura A., Wada Y., and Ohsumi Y. Novel system for monitoring autophagy in the yeast Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 210 (1995) 126-132
19. Ogata M., Hino S., Saito A., Morikawa K., Kondo S., Kanemoto S., Murakami T., Taniguchi M., Tanii I., Yoshinaga K., Shiosaka S., Hammarback J.A., Urano F., and Imaizumi K. Autophagy is activated for cell survival after endoplasmic reticulum stress. Mol. Cell Biol. 26 (2006) 9220-9231
20. Ohsumi Y., and Mizushima N. Two ubiquitin-like conjugation systems essential for autophagy. Semin. Cell Dev. Biol. 15 (2004) 231-236
21. Overbye A., Fengsrud M., and Seglen P.O. Proteomic analysis of membrane-associated proteins from rat liver autophagosomes. Autophagy 3 (2007) 300-322
22. Reitzer L.J., Wice B.M., and Kennell D. Evidence that glutamine, not sugar, is the major energy source for cultured HeLa cells. J. Biol. Chem. 254 (1979) 2669-2676
23. Sanjuan M.A., Dillon C.P., Tait S.W., Moshiach S., Dorsey F., Connell S., Komatsu M., Tanaka K., Cleveland J.L., Withoff S., and Green D.R. Toll-like receptor signalling in macrophages links the autophagy pathway to phagocytosis. Nature 450 (2007) 1253-1257
24. Schellens J.P., and Meijer A.J. Energy depletion and autophagy. Cytochemical and biochemical studies in isolated rat hepatocytes. Histochem. J. 23 (1991) 460-466
25. Shintani T., and Klionsky D.J. Cargo proteins facilitate the formation of transport vesicles in the cytoplasm to vacuole targeting pathway. J. Biol. Chem. 279 (2004) 29889-29894
26. Ueno T., Ishidoh K., Mineki R., Tanida I., Murayama K., Kadowaki M., and Kominami E. Autolysosomal membrane-associated betaine homocysteine methyltransferase. Limited degradation fragment of a sequestered cytosolic enzyme monitoring autophagy. J. Biol. Chem. 274 (1999) 15222-15229
27. Yorimitsu T., Nair U., Yang Z., and Klionsky D.J. Endoplasmic reticulum stress triggers autophagy. J. Biol. Chem. 281 (2006) 30299-30304
28. Zhu W., Cowie A., Wasfy G.W., Penn L.Z., Leber B., and Andrews D.W. Bcl-2 mutants with restricted subcellular location reveal spatially distinct pathways for apoptosis in different cell types. EMBO J. 15 (1996) 4130-4141
29. Zong W.X., Li C., Hatzivassiliou G., Lindsten T., Yu Q.C., Yuan J., and Thompson C.B. Bax and Bak can localize to the endoplasmic reticulum to initiate apoptosis. J. Cell Biol. 162 (2003) 59-69