Microwave-assisted rapid plant sample preparation for transmission electron microscopy

Journal of Microscopy

Volumn 233, Issue 2, 2009, Pages 258-268

  Zechmann, B ^a   Zellnig, G ^a  

^a UNIVERSITY OF GRAZ   (Austria)

Author keywords

Arabidopsis; Chloroplast; Image analysis; Microwave assisted sample preparation; Nicotiana; Nucleus; Picea; Plasmamembrane; Transmission electron microscopy

Indexed keywords

AUTOMATION; CELL MEMBRANES; ELECTRONS; HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; MICROSTRUCTURE; PLANTS (BOTANY); RESINS;

ARABIDOPSIS; CHLOROPLAST; IMAGE-ANALYSIS; MICROWAVE ASSISTED SAMPLE PREPARATION; MICROWAVE-ASSISTED; NICOTIANA; NUCLEUS; PICEA; PLASMAMEMBRANE; SAMPLE PREPARATION;

TISSUE;

ARABIDOPSIS; ARTICLE; AUTOMATION; CONTROLLED STUDY; ELECTRON MICROSCOPE; IMAGE PROCESSING; NONHUMAN; NORWAY SPRUCE; PLANT LEAF; POLYMERIZATION; PRIORITY JOURNAL; TOBACCO; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

ARABIDOPSIS; ARABIDOPSIS THALIANA; NICOTIANA; NICOTIANA TABACUM; PICEA; PICEA ABIES;

EID: 59149088667     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2009.03116.x     Document Type: Article

References (37)

1. Al-Amoudi, A., Dubochet, J., Gnaegi, H., Luthi, W. & Studer, D. (2003) An oscillating cryo-knife reduces cutting-induced deformation of vitreous ultrathin sections. J. Microsc. 212, 26 33.
2. Al-Amoudi, A., Dubochet, J. & Norlén, L. (2005) Nanostructure of the epidermal extracellular space as observed by cryo-electron microscopy of vitreous sections of human skin. J. Investig. Dermatol. 124, 764 777.
3. Benhamou, N., Noel, S., Grenier, J. & Asselin, A. (1991) Microwave energy fixation of plant tissue: an alternative approach that provides excellent preservation of ultrastructure and antigenicity. J. Electron Microsc. Tech. 17, 81 94.
4. Bortz, J., Lienert, G.A. & Boehnke, K. (2000) Verteilungsfreie Methoden in der Biostatistik. Springer Verlag, Berlin.
5. Bouchet-Marquis, C., Dubochet, J. & Fakan, S. (2006) Cryoelectron microscopy of vitrified sections: a new challenge for the analysis of functional nuclear architecture. Histochem. Cell Biol. 125, 43 51.
6. Bouchet-Marquis, C., Zuber, B., Glynn, A.M. et al 2007) Visualization of cell microtubules in their native state. Biol. Cell 99, 45 53.
7. Bozzola, J.J. & Russell, D. (1999) Electron Microscopy. Jones and Barlett Publishers, Boston.
8. De la Hoz, A., Diaz-Ortiz, A. & Moreno, A. (2005) Microwaves in organic synthesis. Thermal and non-thermal microwave effects. Chem. Soc. Rev. 34 (2 164 178.
9. Ebel, B., Hamelmann, U. & Nieß, C. (1990) A rapid preparation method for ultrastructural investigations of conifer needles. J. Microsc. 160, 67 74.
10. Giberson, R.T. & Demaree Jr., R.S. (1999) Microwave processing techniques for electron microscopy: a four-hour protocol. Meth. Molec. Biol., 145 158.
11. Giberson, R.T., Demaree Jr., R.S. & Nordhausen, R.W. (1997) Four-hour processing of clinical/diagnostic specimens for electron microscopy using microwave technique. J. Vet. Diagn. Invest. 9, 61 67.
12. Giddings, T.H. (2003) Freeze-substitution protocols for improved visualization of membranes in high-pressure frozen samples. J. Microsc. 212, 53 61.
13. Hall, J.L. & Hawes, C. (1991) Electron Microscopy of Plant Cells. Academic Press, Harcourt, Brace Jovanovich Publishers, London.
14. Hess, M.W. (2007) Cryopreparation methodology for plant cell biology. Meth. Cell Biol. 79, 57 100.
15. Heumann, H.G. (1992) Microwave-stimulated glutaraldehyde and osmium tetroxide fixation of plant tissue: ultrastructural preservationin seconds. Histochem 97, 341 347.
16. Kaneko, Y. & Walther, P. (1995) Comparison of ultrastructure of germinating pea leaves prepared by high-pressure freezing-freeze substitution and conventional chemical fixation. J. Electron. Microsc. 44, 104 109.
17. Kang, Z., Rohringer, R., Chong, J. & Haber, S. (1991) Microwave fixation of rust-infected wheat leaves. Protoplasma 162, 27 37.
18. Kiss, J.Z, Giddings Jr., T.H., Staehelin, L.A. & Sack, F.D. (1990) Comparison of the ultrastructure of conventionally fixed and high-pressure frozen/freeze- substituted root tips of Nicotiana and Arabidopsis. Protoplasma 157, 64 74.
19. Kok, L.P. & Boon, M.E. (2003) Microwaves for the Art of Microscopy. Coulomb Press Leyden, Leiden.
20. Kok, L.P., Boon, M.E. & Smid, H.M. (1993) The problem of hot spots in microwave equipment used for preparatory techniques- theory and practice. Scanning 15, 100 109.
21. Kuo, J. (2007) Processing plant tissues for ultrastructural study. Methods in Molecular Biology, Electron Microscopy, Methods and Protocols (ed. by J. Kuo vol. 369, pp. 47 65. Humana Press, Totowa, New Jersey.
22. Laue, M., Niederwöhrmeier, B. & Bannert, N. (2007) Rapid diagnostic thin section electron microscopy of bacterial endospores. J. Microbiol. Meth. 70, 45 54.
23. Lería, F., Marco, R. & Medina, F.J. (2004) Structural and antigenic preservation of plant samples by microwave-enhanced fixation, using dedicated hardware, minimizing heat-related effects. Micr. Res. Techniq. 65, 86 100.
24. Leong, A.S.Y. & Sormunen, R.T. (1998) Microwave procedures for electron microscopy and resin-embedded sections. Micron 29, 397 409.
25. Login, G.R., Ku, T.C. & Dvorak, A.M. (1995) Rapid primary microwave-aldehyde and microwave-osmium fixation: improved detection of rat parotid acinar cell secretory granule a-amylase using a post-embedding immunogold ultrastructural morphometric analysis. J. Histochem. Cytochem. 43, 515 523.
26. Meyberg, M., Lockhausen, J. & Kristen, U. (1988) Ultrastructural changes in mesophyll cells of spruce needles from a declining forest in northern Germany. Eur. J. For. Path. 18, 169 175.
27. Newcomb, W., Jackson, S., Racette, S. & Torrey, J.G. (1990) Ultrastructure of infected cells in the actinorhizal root nodules of Gymnostoma papuanum (Casuarinaceae) prepared by high-pressure freezing and chemical fixation. Protoplasma 157, 172 181.
28. Pfeiffer, S. & Krupinska, K. (2005) New insights in thylakoid membrane organization. Plant Cell Physiol. 46, 1443 1451.
29. Schroeder, J.A., Gelderblom, H.R., Hauroeder, B., Schmetz, C., Milios, J. & Hofstaedter, F. (2006) Microwave-assisted tissue processing for same-day EM-diagnosis of potential bioterrorism and clinical samples. Micron 37, 577 590.
30. Sørensen, S. (1909) Über die Messung und die Bedeutung der Wasserstoffionenkonzentration bei Enzymatischen prozessen (mit einer ergänzung). Biochem. Zeit. 21, 131 304.
31. Tiedemann, J., Hohenberg, H. & Kollmann, R. (1998) High-pressure freezing of plant cells cultured in cellulose microcapillaries. J. Microsc. 189, 163 171.
32. Vanhecke, D., Studer, L. & Studer, D. (2007) Cryoelectron microscopy of vitreous sections. Methods in Molecular Biology, Electron Microscopy, Methods and Protocols (ed. by J. Kuo vol. 369, pp. 175 197. Humana Press, Totowa, New Jersey.
33. Walther, P. & Ziegler, A. (2002) Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution media contains water. J. Microsc. 208, 3 10.
34. Webster, P. (2007) Microwave-assisted processing and embedding for transmission electron microscopy. Methods in Molecular Biology, Electron Microscopy, Methods and Protocols (ed. by J. Kuo vol. 369, pp. 47 65. Humana Press, Totowa, New Jersey.
35. Wendt, K.D., Jensen, C.A., Tindall, R. & Katz, M.L. (2004) Comparison of conventional and microwave-assisted processing of mouse retinas for transmission electron microscopy. J. Microsc. 214, 80 88.
36. Zechmann, B., Müller, M. & Zellnig, G. (2005) Effects of different fixation and freeze substitution methods on the ultrastructural preservation of ZYMV-infected Cucurbita pepo (L.) leaves. J. Electron Microsc. 54, 393 402.
37. Zechmann, B., Müller, M. & Zellnig, G. (2007) Membrane associated qualitative differences in cell ultrastructure of chemically and high pressure cryofixed plant cells. J. Struct. Biol. 158, 370 377.