The use of "lab-on-a-chip" microfluidic SDS electrophoresis technology for the separation and quantification of milk proteins

International Dairy Journal

Volumn 19, Issue 4, 2009, Pages 198-204

  Anema, Skelte G ^a  

^a FONTERRA RESEARCH CENTRE   (New Zealand)

Author keywords

[No Author keywords available]

Indexed keywords

BODY FLUIDS; CASEIN; DIFFUSERS (OPTICAL); ELECTROCHEMISTRY; ELECTROPHORESIS; FLUIDIC DEVICES; GELATION; POLYMERS; PROTEINS; SEPARATION; SODIUM;

BOVINE SERUM ALBUMINS; ELECTROPHORESIS TECHNOLOGIES; LAB ON A CHIPS; LACTOFERRIN; LACTOGLOBULIN; MICROFLUIDIC CHIPS; MILK PROTEINS; MILK SAMPLES; SDS PAGES; SODIUM DODECYL SULPHATE; STANDARD CURVES; STANDARD PROTEINS; WHEY PROTEINS;

BIOCHIPS;

BOVINAE;

EID: 59049094510     PISSN: 09586946     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.idairyj.2008.11.001     Document Type: Article

References (40)

1. 2-caseins. Milchwissenschaft - Milk Science International 45 (1990) 708-711
2. Andrews A.T. Proteinases in normal bovine milk and their action on caseins. Journal of Dairy Research 50 (1983) 45-55
3. Andrews A.T., Taylor M.D., and Owen A.J. Rapid analysis of bovine milk proteins by fast protein liquid chromatography. Journal of Chromatography 348 (1985) 177-185
4. Anema S.G., Lee S.K., and Klostermeyer H. Effect of protein, nonprotein-soluble components, and lactose concentrations on the irreversible thermal denaturation of β-lactoglobulin and α-lactalbumin in skim milk. Journal of Agricultural and Food Chemistry 54 (2006) 7339-7348
5. Anema S.G., and Lloyd R.J. Analysis of whey protein denaturation: a comparative study of alternative methods. Milchwissenschaft 54 (1999) 206-210
6. Anema S.G., and McKenna A.B. Reaction kinetics of thermal denaturation of whey proteins in heated reconstituted whole milk. Journal of Agricultural and Food Chemistry 44 (1996) 422-428
7. Bobe G., Beitz D.C., Freeman A.E., and Lindberg G.L. Sample preparation affects separation of whey proteins by reversed-phase high-performance liquid chromatography. Journal of Agricultural and Food Chemistry 46 (1998) 1321-1325
8. Bobe G., Beitz D.C., Freeman A.E., and Lindberg G.L. Separation and quantification of bovine milk proteins by reversed-phase high-performance liquid chromatography. Journal of Agricultural and Food Chemistry 46 (1998) 458-463
9. Braun F., Krause I., and Klostermeyer H. Efficient determination of skim milk powder, casein, whey protein and total milk protein in compound feedingstuffs by isoelectric focusing and laser densitometry. Milchwissenschaft 45 (1990) 3-9
10. Butikofer U., Meyer J., and Rehberger B. Determination of the percentage of α-lactalbumin and β-lactoglobulin of total milk protein in raw and heat treated skim milk. Milchwissenschaft 61 (2006) 263-266
11. Chaplin L.C. Hydrophobic interaction fast protein liquid chromatography of milk proteins. Journal of Chromatography 363 (1986) 329-335
12. Chen F.T.A., and Zang J.H. Determination of milk proteins by capillary electrophoresis. Journal of AOAC International 75 (1992) 905-909
13. s1-casein A, B and C variants by plasmin and chymosin. International Dairy Journal 9 (1999) 371-372
14. Creamer L.K. Electrophoresis of cheese. International Dairy Federation, IDF Bulletin 261 (1991) 14-28
15. s1- and β-caseins on gel electrophoresis in sodium dodecyl sulfate buffers. Archives of Biochemistry and Biophysics 234 (1984) 476-486
16. Dannenberg F., and Kessler H.G. Quantitative densitometry of heat-induced changes in whey proteins following ultrathin-layer isoelectric focusing. Electrophoresis 7 (1986) 67-72
17. Dupont D., and Muller-Renaud S. Quantification of proteins in dairy products using an optical biosensor. Journal of AOAC International 89 (2006) 843-848
18. Farrell H.M., Jimenez-Flores R., Bleck G.T., Brown E.M., Butler J.E., Creamer L.K., Hicks C.L., Hollar C.M., Ng-Kwai-Hang K.F., and Swaisgood H.E. Nomenclature of the proteins of cows' milk - sixth revision. Journal of Dairy Science 87 (2004) 1641-1674
19. Fasman G.D. Practical Handbook of Biochemistry and Molecular Biology (1989), CRC Press, Boca Raton, FL, USA
20. Goetz H., Kuschel M., Wulff T., Sauber C., Miller C., Fisher S., and Woodward C. Comparison of selected analytical techniques for protein sizing, quantitation and molecular weight determination. Journal of Biochemical and Biophysical Methods 60 (2004) 281-293
21. Hey J.S. Advances in microscale protein fractionation and analysis. American Biotechnology Laboratory 25 (2007) 12-16
22. Hillier R.M. The quantitative measurement of whey proteins using polyacrylamide-gel electrophoresis. Journal of Dairy Research 43 (1976) 259-265
23. Hurley W.L. Immunoglobulins in mammary secretions. In: Fox P.F., and McSweeney P.L.H. (Eds). Proteins. third ed. Advanced Dairy Chemistry vol. 1 (2003), Kluwer Academic/Plenum Publishers, New York, NY, USA 422-447
24. Jeong S., Biondi S., Mikkelsen J., Fathollahi B., Winoto A., Spaid M., and Chow A. An automated alternative to SDS-PAGE for protein analysis. American Biotechnology Laboratory 23 (2005) 8-10
25. de Jong N., Visser S., and Olieman C. Determination of milk-proteins by capillary electrophoresis. Journal of Chromatography A 652 (1993) 207-213
26. Kessler H.G., and Beyer H.J. Thermal denaturation of whey proteins and its effect in dairy technology. International Journal of Biological Macromolecules 13 (1991) 165-173
27. Lonnerdal B. Lactoferrin. In: Fox P.F., and McSweeney P.L.H. (Eds). Proteins. third ed. Advanced Dairy Chemistry vol. 1 (2003), Kluwer Academic/Plenum Publishers, New York, NY, USA 449-466
28. Lyster L.J. The denaturation of α-lactalbumin and β-lactoglobulin in heated milk. Journal of Dairy Research 37 (1970) 233-243
29. Ng-Kwai-Hang K.F., and Kroeker E.M. Rapid separation and quantification of major caseins and whey proteins of bovine milk by polyacrylamide gel electrophoresis. Journal of Dairy Science 67 (1984) 3052-3056
30. Ohashi R., Otero J.M., Chwistek A., and Hamel J.F.P. Determination of monoclonal antibody production in cell culture using novel microfluidic and traditional assays. Electrophoresis 23 (2002) 3623-3629
31. Patel H.A., Anema S.G., Holroyd S.E., Singh H., and Creamer L.K. Methods to determine denaturation and aggregation of proteins in low-, medium- and high-heat skim milk powders. Lait 87 (2007) 251-268
32. Poynton T.A. EZAnalyze. (Version 3.0) [Computer Software and Manual] (2007). http://www.ezanalyze.com (accessed 24.01.08.)
33. Smith C. Striving for purity: advances in protein purification. Nature Methods 23 (2005) 8-10
34. Strange E.D., Vanhekken D., and Thompson M.P. Qualitative and quantitative determination of caseins with reverse-phase and anion-exchange HPLC. Journal of Food Science 56 (1991) 1415-1420
35. Swaisgood H.E. Chemistry of milk protein. In: Fox P.F. (Ed). Proteins. Developments in Dairy Chemistry vol. 1 (1982), Elsevier Applied Science, London, UK 1-59
36. Swaisgood H.E. Chemistry of the caseins. In: Fox P.F. (Ed). Proteins. third ed. Advanced Dairy Chemistry vol. 1 (1992), Elsevier Applied Science, London, UK 63-110
37. Trieu-Cuot P., and Gripon J.C. Electrofocusing and two-dimensional electrophoresis of bovine caseins. Journal of Dairy Research 48 (1981) 303-310
38. Visser S., Slangen C.J., and Rollema H.S. Phenotyping of bovine milk proteins by reversed phase high performance liquid chromatography. Journal of Chromatography 548 (1991) 361-370
39. Walstra P., and Jenness R. Dairy Chemistry and Physics (1984), John Wiley and Sons, New York, NY, USA
40. Wu D., Qin J., and Lin B. Electrophoretic separations on microfluidic chips. Journal of Chromatography A 1184 (2008) 542-559