Enterotoxigenic Escherichia coli EtpA mediates adhesion between flagella and host cells

Nature

Volumn 457, Issue 7229, 2009, Pages 594-598

  Roy, Koushik ^a   Hilliard, George M ^a   Hamilton, David J ^a   Luo, Jiwen ^a   Ostmann, Marguerite M ^b   Fleckenstein, James M ^a,b  

^a UNIVERSITY OF TENNESSEE HEALTH SCIENCE CENTER   (United States)

^b VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESIN; ESCHERICHIA COLI ENTEROTOXIN; ETPA PROTEIN; FLAGELLIN; UNCLASSIFIED DRUG;

COLIFORM BACTERIUM; DEVELOPING WORLD; DIARRHEAL DISEASE; HOST-PATHOGEN INTERACTION; SECRETION; TOXICOLOGY; VACCINE; VIRULENCE;

ARTICLE; BACTERIAL COLONIZATION; BACTERIUM ADHERENCE; CELL ADHESION; DIARRHEA; ENTEROTOXIGENIC ESCHERICHIA COLI; EPITHELIUM CELL; FLAGELLUM; HOST CELL; INTESTINE FLORA; NONHUMAN; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN INTERACTION;

ANIMALS; BACTERIAL ADHESION; BACTERIAL VACCINES; CELL LINE; CONSERVED SEQUENCE; ENTEROTOXIGENIC ESCHERICHIA COLI; EPITHELIAL CELLS; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI PROTEINS; FLAGELLA; FLAGELLIN; HOST-PATHOGEN INTERACTIONS; INTESTINE, SMALL; MEMBRANE GLYCOPROTEINS; MICE; PROTEIN BINDING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; NEGIBACTERIA;

EID: 59049093883     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature07568     Document Type: Article

References (29)

1. Torres, A. G., Zhou, X. & Kaper, J. B. Adherence of diarrheagenic Escherichia coli strains to epithelial cells. Infect. Immun. 73, 18-29 (2005).
2. Dorsey, F. C., Fischer, J. F. & Fleckenstein, J. M. Directed delivery of heat-labile enterotoxin by enterotoxigenic Escherichia coli. Cell. Microbiol. 8, 1516-1527 (2006).
3. World Health Organization. Future directions for research on enterotoxigenic Escherichia coli vaccines for developing countries. Wkly Epidemiol. Rec. 81, 97-104 (2006).
4. Qadri, F. et al. Enterotoxigenic Escherichia coli in developing countries: epidemiology, microbiology, clinical features, treatment, and prevention. Clin. Microbiol. Rev. 18, 465-483 (2005).
5. Jacob-Dubuisson, F., Locht, C. & Antoine, R. Two-partner secretion in Gram-negative bacteria: a thrifty, specific pathway for large virulence proteins. Mol. Microbiol. 40, 306-313 (2001).
6. Fleckenstein, J. M. et al. Identification of a two-partner secretion locus of enterotoxigenic Escherichia coli. Infect. Immun. 74, 2245-2258 (2006).
7. Yonekura, K., Maki-Yonekura, S. & Namba, K. Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy. Nature 424, 643-650 (2003).
8. Evans, D. G. et al. Plasmid-controlled colonization factor associated with virulence in Escherichia coli enterotoxigenic for humans. Infect. Immun. 12, 656-667 (1975).
9. Gaastra, W. & Svennerholm, A. M. Colonization factors of human enterotoxigenic Escherichia coli (ETEC). Trends Microbiol. 4, 444-452 (1996).
10. Svennerholm, A. M. & Tobias, J. Vaccines against enterotoxigenic Escherichia coli. Expert Rev. Vaccines 7, 795-804 (2008).
11. Giron, J. A. et al. The flagella of enteropathogenic Escherichia coli mediate adherence to epithelial cells. Mol. Microbiol. 44, 361-379 (2002).
12. Wolf, M. K. Occurrence, distribution, and associations of O and H serogroups, colonization factor antigens, and toxins of enterotoxigenic Escherichia coli. Clin. Microbiol. Rev. 10, 569-584 (1997).
13. Yonekura, K. et al. The bacterial flagellar cap as the rotary promoter of flagellin self-assembly. Science 290, 2148-2152 (2000).
14. Sudakin, V. in Protein-Protein Interactions: A Molecular Cloning Manual 2nd edn, Vol. 1 (eds Golemis, E. & Adams, P. D.) Ch. 3 37-54 (Cold Spring Harbor Laboratory Press, 2005).
15. Altschul, S. F. et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389-3402 (1997).
16. Komoriya, K. et al. Flagellar proteins and type III-exported virulence factors are the predominant proteins secreted into the culture media of Salmonella typhimurium. Mol. Microbiol. 34, 767-779 (1999).
17. Jin, Q. & He, S. Y. Role of the Hrp pilus in type III protein secretion in Pseudomonas syringae. Science 294, 2556-2558 (2001).
18. Maki-Yonekura, S., Yonekura, K. & Namba, K. Domain movements of HAP2 in the cap-filament complex formation and growth process of the bacterial flagellum. Proc. Natl Acad. Sci. USA 100, 15528-15533 (2003).
19. Goff, S. P. & Prasad, V. R. Linker insertion mutagenesis as probe of structure-function relationships. Methods Enzymol. 208, 586-603 (1991).
20. Roy, K. et al. The EtpA exoprotein of enterotoxigenic Escherichia coli promotes intestinal colonization and is a protective antigen in an experimental model of murine infection. Infect. Immun. 76, 2106-2112 (2008).
21. Ottemann, K. M. & Miller, J. F. Roles for motility in bacterial-host interactions. Mol. Microbiol. 24, 1109-1117 (1997).
22. Plotkin, S. A. Vaccines: correlates of vaccine-induced immunity. Clin. Infect. Dis. 47, 401-409 (2008).
23. Mu, X. Q., Savarino, S. J. & Bullitt, E. The three-dimensional structure of CFA/I adhesion pili: traveler's diarrhea bacteria hang on by a spring. J. Mol. Biol. 376, 614-620 (2008).
24. Buscher, A. Z. et al. Surface anchoring of a bacterial adhesin secreted by the two-partner secretion pathway. Mol. Microbiol. 61, 470-483 (2006).
25. Smith, K. D. et al. Toll-like receptor 5 recognizes a conserved site on flagellin required for protofilament formation and bacterial motility. Nature Immunol. 4, 1247-1253 (2003).
26. Allen, K. P., Randolph, M. M. & Fleckenstein, J. M. Importance of heat-labile enterotoxin in colonization of the adult mouse small intestine by human enterotoxigenic Escherichia coli strains. Infect. Immun. 74, 869-875 (2006).
27. Cummings, E. D. et al. High-throughput proteomics processing of proteins in polyacrylamide in a multiwell format. J. Proteome Res. 6, 1603-1608 (2007).
28. Zhang, W. & Chait, B. T. ProFound: an expert system for protein identification using mass spectrometric peptide mapping information. Anal. Chem. 72, 2482-2489 (2000).
29. Hayashi, F. et al. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature 410, 1099-1103 (2001).