Gene expression normalization in a dual-compartment system: A real-time quantitative polymerase chain reaction protocol for symbiotic anthozoans

Molecular Ecology Resources

Volumn 9, Issue 2, 2009, Pages 462-470

  Mayfield, Anderson B ^a   Hirst, Marissa B ^a   Gates, Ruth D ^a  

^a NONE

Author keywords

Anthozoan; Coral reefs; Real time quantitative PCR; Reference gene; Symbiosis

Indexed keywords

ACTINIARIA; AIPTASIA PULCHELLA; ANTHOZOA; DINOPHYCEAE; SYMBIODINIUM;

EID: 58849143153     PISSN: 1755098X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1755-0998.2008.02349.x     Document Type: Article

References (32)

1. Allemand D, Ferrier-Pagès C, Furla P et al 2004) Biomineralisation in reef-building corals: from molecular mechanisms to environmental control. Comptes Rendus de Lí'academie Des Sciences, Paris Palevology, 3, 453 467.
2. Bower NI, Moser RJ, Hill JR, Lehnert SA (2007) Universal reference method for real-time PCR gene expression analysis of preimplantation embryos. BioTechniques, 42, 199 206.
3. Brown BE (1997) Coral bleaching: causes and consequences. Coral Reefs, 16, 129 138.
4. Bustin SA (2002) Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. Journal of Molecular Endocrinology, 29, 23 39.
5. Bustin SA (2004) A-Z of Quantitative PCR. International University Line, La Jolla, California.
6. Dempster EL, Pryor KV, Francis D, Young JE, Roger HJ (1999) Rapid DNA extraction from ferns for PCR-based analyses. BioTechniques, 27, 66 68.
7. Drew EA (1972) The biology and physiology of algae-invertebrate symbiosis II. The density of symbiotic algal cells in a number of hermatypic hard corals and alcyonarians from various depths. Journal of Experimental Marine Biology and Ecology, 9, 71 75.
8. Edmunds PJ, Gates RD (2002) Normalizing physiological data for scleractinian corals. Coral Reefs, 21, 193 197.
9. Edmunds PJ, Gates RD (2008) Acclimatization in tropical reef corals. Marine Ecology Progress Series, 361, 307 310.
10. Gates RD (1990) Seawater temperature and sublethal coral bleaching in Jamaica. Coral Reefs, 8, 193 197.
11. Gates RD, Edmunds PJ (1999) The physiological mechanisms of acclimatization in tropical reef corals. American Zoologist, 39, 30 43.
12. Hoegh-Guldberg O (1999) Climate change, coral bleaching and the future of the world's coral reefs. Marine and Freshwater Research, 50, 839 866.
13. Hoegh-Guldberg O, Mumby PJ, Hooten AJ et al 2007) Coral reefs under rapid climate change and acidification. Science, 318, 1737 1743.
14. Hofmann GE, Burnaford JL, Fielman KT (2005) Genomics-fueled approaches to current challenges in marine ecology. Trends in Ecology & Evolution, 20, 305 311.
15. Huggett J, Dheda K, Bustin S, Zumla A (2005) Real-time RT-PCR normalization; strategies and considerations. Genes and Immunity, 6, 279 284.
16. Hummon AB, Lim SR, Difilippantonio MJ, Ried T (2007) Isolation and solubilization of proteins after TRIzol® extraction of RNA and DNA from patient material following prolonged storage. BioTechniques, 42, 467 472.
17. Johnson DR, Lee PKH, Holmes VF, Alvarez-Cohen L (2005) An internal reference technique for accurately quantifying specific mRNAs by real-time PCR with application to the tceA reductive dehalogenase gene. Applied and Environmental Microbiology, 71, 3866 3871.
18. LaJeunesse T (2005) 'Species' radiations of symbiotic dinoflagellates in the Atlantic and Indo-Pacific since the Miocene-Pliocene transition. Molecular Biology and Evolution, 22, 570 581.
19. Leggat W, Hoegh-Guldberg O, Dove S, Yellowlees D (2007) Analysis of an EST library from the dinoflagellate (Symbiodinium sp.) symbiont of reef-building corals. Journal of Phycology, 43, 1010 1021.
20. Loram JE, Boonham N, O'Toole P, Trapido-Rosenthal HG, Douglas AE (2007a) Molecular quantification of symbiotic dinoflagellate algae of the genus Symbiodinium. Biological Bulletin, 212, 259 268.
21. Loram JE, Trapido-Rosenthal HG, Douglas AE (2007b) Functional significance of genetically different symbiotic algae, Symbiodinium, in a coral reef symbiosis. Molecular Ecology, 16, 4849 4857.
22. Mayfield AB, Gates RD (2007) Osmoregulation in anthozoan-dinoflagellate symbiosis. Comparative Biochemistry and Physiology, 147A, 1 10.
23. Mieog JC, van Oppen MJH, Cantin NE, Stam WT, Olsen JL (2007) Real-time PCR reveals a high incidence of Symbiodinium clade D at low levels in four scleractinian corals across the Great Barrier Reef: implications for symbiont shuffling. Coral Reefs, 26, 449 457.
24. Moya A, Tambutte S, Beranger G et al 2008) Cloning and use of a coral 36B4 gene to study the differential expression of coral genes between light and dark conditions. Marine Biotechnology, Doi.
25. Muscatine L, Porter JW (1977) Reef corals: mutualistic symbioses adapted to nutrient poor environments. Bioscience, 27, 454 460.
26. van Oppen MJH, Gates RD (2006) Conservation genetics and the resilience of reef-building corals. Molecular Ecology, 15, 3863 3883.
27. Rodriguez-Lanetty M, Phillips WS, Dove S, Hoegh-Guldberg O, Weis VM (2008) Analytical approach for selecting normalizing genes from a cDNA microarray platform to be used in q-RT PCR assays: a cnidarian case study. Journal of Biochemical and Biophysical Methods, 70, 985 991.
28. Smith-Keune C, Dove S (2007) Gene expression of a green fluorescent protein homolog as a host-specific biomarker of heat stress within a reef-building coral. Marine Biotechnology, 10, 166 180.
29. Stat M, Carter D, Hoegh-Guldberg O (2006) The evolutionary history of Symbiodinium and scleractinian hosts-symbiosis, diversity, and the effect of climate changes. Perspectives in Plant Ecology, Evolution and Systematics, 8, 23 43.
30. Tricarico C, Pinzani P, Bianchi S, Paglierani M, Distante V, Pazzagli M, Bustin SA, Orlando C (2002) Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Annals of Biochemistry, 309, 293 300.
31. Yellowlees D, Rees TA, Leggat W (2008) Metabolic interactions between algal symbionts and invertebrate hosts. Plant Cell and Environment, 31, 679 694.
32. Zhu Y, Song D, Tran NT, Nguyen N (2007) The effects of members of growth hormone family knockdown in zebrafish development. General and Comparative Endocrinology, 150, 395 404.