Oligonucleotide-mediated retroviral RNase H activation leads to reduced HIV-1 titer in patient-derived plasma

AIDS

Volumn 23, Issue 2, 2009, Pages 213-221

  Heinrich, Jochen ^a   Mathur, Srikanth ^a   Matskevich, Alexey A ^a   Moelling, Karin ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

Antiretroviral strategy; HIV; Oligodeoxynucleotides; Patients; RNase H activation

Indexed keywords

OLIGODEOXYNUCLEOTIDE A; OLIGODEOXYNUCLEOTIDE DERIVATIVE; RIBONUCLEASE H; SAQUINAVIR; UNCLASSIFIED DRUG; VIRUS RNA; ZIDOVUDINE;

AFRICA; ANTIVIRAL ACTIVITY; ARTICLE; BLOOD SAMPLING; CLINICAL ARTICLE; ENZYME ACTIVATION; EX VIVO STUDY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA DEGRADATION; VIRION; VIRUS INFECTIVITY; VIRUS ISOLATION; VIRUS LOAD; VIRUS STRAIN;

BASE SEQUENCE; CELLS, CULTURED; DNA, VIRAL; DRUG RESISTANCE, VIRAL; ENZYME ACTIVATION; GENE THERAPY; HIV INFECTIONS; HIV-1; HUMANS; LEUKOCYTES, MONONUCLEAR; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDES, ANTISENSE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RIBONUCLEASE H; RNA, VIRAL; VIRAL LOAD; VIRION; VIRUS INACTIVATION;

EID: 58849139999     PISSN: 02699370     EISSN: None     Source Type: Journal    
DOI: 10.1097/QAD.0b013e32831c5480     Document Type: Article

References (16)

1. Wohrl BM, Moelling K. Interaction of HIV-1 ribonuclease H with polypurine tract containing RNA-DNA hybrids. Biochemistry 1990; 29:10141-10147.
2. Jendis J, Strack B, Volkmann S, Boni J, Moelling K. Inhibition of replication of fresh HIV type 1 patient isolates by a polypurine tract-specific self-complementary oligodeoxynucleotide. AIDS Res Hum Retroviruses 1996; 12: 1161- 1168.
3. Jendis J, Strack B, Moelling K. Inhibition of replication of drug- resistant HIV type 1 isolates by polypurine tract-specific oligodeoxynucleotide TFO A. AIDS Res Hum Retroviruses 1998; 14:999-1005.
4. Moelling K, Abels S, Jendis J, Matskevich A, Heinrich J. Silencing of HIV by hairpin-loop-structured DNA oligonucleotide. FEBS Lett 2006; 580:3545-3550.
5. Matskevich AA, Ziogas A, Heinrich J, Quast S, Moelling K. Short partially double-stranded oligodeoxynucleotide induces reverse transcriptase-mediated cleavage of HIV RNA and abrogates infectivity of virions. AIDS Res Hum Retroviruses 2006; 22:1220-1230.
6. Hansen J, Schulze T, Mellert W, Moelling K. Identification and characterization of HIV-specific RNase H by monoclonal antibody. EMBO J 1988; 7:239-243.
7. Moelling K, Bolognesi DP, Bauer H, Busen W, Plassmann HW, Hausen P. Association of viral reverse transcriptase with an enzyme degrading the RNA moiety of RNA-DNA hybrids. Nat New Biol 1971; 234:240-243.
8. Matzen K, Elzaouk L, Matskevich A, Nitzsche A, Heinrich J, Moelling K. RNase H-induced suicide of a retrovirus by oligo- deoxynucleotides in a mouse model. Nat Biotechnol 2007; 25:669-674.
9. Brown BK, Darden JM, Tovanabutra S, Oblander T, Frost J, Sanders-BueII E, et al. Biologic and genetic characterization of a panel of 60 human immunodeficiency virus type 1 isolates, representing clades A, B, C, D, CRF01-AE, and CRF02-AG, for the development and assessment of candidate vaccines. J Virol 2005; 79:6089-6101.
10. Volkmann S, Jendis J, Frauendorf A, Moelling K. Inhibition of HIV-1 reverse transcription by triple-helix forming oligonucleotides with viral RNA. Nucleic Acids Res 1995; 23:1204- 1212.
11. Fransen K, Mortier D, Heyndrickx L, Verhofstede C, Janssens W, van der Croen G. Isolation of HIV-1 RNA from plasma: evaluation of seven different methods for extraction (part two). J Virol Methods 1998; 76: 153-157.
12. Bisset LR, Bosbach S, Tomasik Z, Lutz H, Schupbach J, Boni J. Quantification of in vitro retroviral replication using a one- tube real-time RT-PCR system incorporating direct RNA preparation. J Virol Methods 2001; 91 : 149-155.
13. GaIIo RC, Wong-StaaI F. A human T-Iymphotropic retrovirus (HTLV-III) as the cause of the acquired immunodeficiency syndrome. Ann Intern Med 1985; 103:679-689.
14. Becker Y. CpG ODNs treatments of HIV-1 infected patients may cause the decline of transmission in high risk populations: a review hypothesis and implications. Virus Genes 2005; 30: 251- 266.
15. Van Aerschot A. Oligonucleotides as antivirals: dream or realistic perspective? Antiviral Res 2006; 71 : 307-316.
16. Burge S, Parkinson GN, Hazel P, Todd AK, Neidle S. Quad- ruplex DNA: sequence, topology and structure. Nucleic Acids Res 2006; 34:5402-5415.