Quantification of in vitro retroviral replication using a one-tube real-time RT-PCR system incorporating direct RNA preparation

Journal of Virological Methods

Volumn 91, Issue 2, 2001, Pages 149-155

  Bisset, Leslie R ^a   Bosbach, Stefan ^a   Tomasik, Zuzanna ^a   Lutz, Hans ^a   Schüpbach, Jörg ^a   Böni, Jürg ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

FIV; HIV; In vitro; Real time; Retrovirus; RNA; RT PCR

Indexed keywords

VIRUS RNA; ZIDOVUDINE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; VIRUS INHIBITION; VIRUS REPLICATION;

ANIMALS; ANTI-HIV AGENTS; ANTIVIRAL AGENTS; CATS; CELL LINE; COSTS AND COST ANALYSIS; DRUG EVALUATION, PRECLINICAL; GENE PRODUCTS, GAG; GENE PRODUCTS, POL; HIV-1; HUMANS; IMMUNODEFICIENCY VIRUS, FELINE; MICROBIAL SENSITIVITY TESTS; POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, VIRAL; TUMOR CELLS, CULTURED; VIRUS REPLICATION; ZIDOVUDINE;

FELINE IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; UNIDENTIFIED RETROVIRUS;

EID: 0035146697     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(00)00259-7     Document Type: Article

References (18)

1. Solvent/detergent treatment of human plasma - A very robust method for virus inactivation
2. Rapid and simple method for purification of nucleic acids
3. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
4. Isolation of HIV-1 RNA from plasma: Evaluation of seven different methods for extraction (part two)
5. RT-PCR without RNA isolation
6. Rapid feline immunodeficiency virus provirus quantitation by polymerase chain reaction using the TaqMan™ fluorogenic real-time detection system
7. Use of real-time PCR and molecular beacons to detect virus replication in human immunodeficiency virus type 1-infected individuals on prolonged effective antiretroviral therapy
8. Advances in quantitative PCR technology: 5′ Nuclease assays
9. Introduction to isolating RNA
10. PCR access! A sensitive single-tube two-enzyme system for RT-PCR
11. Rapid colorometric assay for cellular growth and survival: Application to proliferation and cytotoxicity assay
12. Effects of beta-lactam antibiotics on proliferating eucaryotic cells
13. Identification of the Rev transactivation and Rev-reponsive elements of feline immunodeficiency virus
14. Use of heat release and an internal RNA standard control in reverse transcription-PCR detection of Norwalk virus from stool samples
15. A solvent-free, rapid and simple virus RNA-release method for potato leafroll virus detection in aphids and plants by reverse transcription polymerase chain reaction
16. Freezing of PCR master mixture retains full amplification activity and facilitates PCR standardization for molecular diagnostics and real-time quantitative PCR
17. PCR-based detection of arboviral RNA from mosquitoes homogenized in detergent
18. A new method for extracting DNA or RNA for polymerase chain reaction