Purification and characterization of silkworm hemocytes by flow cytometry

Developmental and Comparative Immunology

Volumn 33, Issue 4, 2009, Pages 439-448

  Nakahara, Yuichi ^a   Shimura, Sachiko ^a   Ueno, Chihiro ^a   Kanamori, Yasushi ^a   Mita, Kazuei ^a   Kiuchi, Makoto ^a   Kamimura, Manabu ^a  

^a NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

Author keywords

Bombyx mori; Cell sorting; Green fluorescence protein; Immunity; Lectin; Paralytic peptide; Pattern recognition protein; Scavenger receptor

Indexed keywords

COMPLEMENTARY DNA; GREEN FLUORESCENT PROTEIN; LECTIN; MONOCLONAL ANTIBODY; PROPIDIUM IODIDE;

ANIMAL CELL; ARTICLE; BLOOD CELL; CELL ISOLATION; CELL POPULATION; CELL SUBPOPULATION; CELLULAR IMMUNITY; FLOW CYTOMETRY; GENE EXPRESSION PROFILING; GRANULOCYTE; HUMORAL IMMUNITY; MOLECULAR PROBE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMA CELL; PRIORITY JOURNAL; SILKWORM;

BOMBYX MORI;

EID: 58649112825     PISSN: 0145305X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.dci.2008.09.005     Document Type: Article

References (49)

1. Williams M.J. Drosophila hemopoiesis and cellular immunity. J Immunol 178 (2007) 4711-4716
2. Wood W., and Jacinto A. Drosophila melanogaster embryonic haemocytes: masters of multitasking. Nat Rev Mol Cell Biol 8 (2007) 542-551
3. Nittono Y. Studies on the blood cells in the silkworm, Bombyx mori L. Bull Sericul Exp Station 16 (1960) 171-266
4. Wago H. Phagocytic recognition in Bombyx mori. In: Gupta A.P. (Ed). Immunology of insects and other arthropods (1991), CRC Press, Boca Raton
5. Akai H., and Sato S. Ultrastructure of the larval hemocytes of the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae). Int J Insect Morphol Embryol 2 (1973) 207-231
6. Yamashita M., and Iwabuchi K. Bombyx mori prohemocyte division and differentiation in individual microcultures. J Insect Physiol 47 (2001) 325-331
7. Iwama R., and Ashida M. Biosynthesis of prophenoloxidase in hemocytes of larval hemolymph of the silkworm, Bombyx mori. Insect Biochem 16 (1986) 547-555
8. Imamura M., Nakahara Y., Kanda T., Tamura T., and Taniai K. A transgenic silkworm expressing the immune-inducible cecropin B-GFP reporter gene. Insect Biochem Mol Biol 36 (2006) 429-434
9. Lavine M.D., and Strand M.R. Insect hemocytes and their role in immunity. Insect Biochem Mol Biol 32 (2002) 1295-1309
10. Ling E., Shirai K., Kanekatsu R., and Kiguchi K. Classification of larval circulating hemocytes of the silkworm, Bombyx mori, by acridine orange and propidium iodide staining. Histochem Cell Biol 120 (2003) 505-511
11. Tirouvanziam R., Davidson C.J., Lipsick J.S., and Herzenberg L.A. Fluorescence-activated cell sorting (FACS) of Drosophila hemocytes reveals important functional similarities to mammalian leukocytes. Proc Natl Acad Sci USA 101 (2004) 2912-2917
12. Tamura T., Thibert C., Royer C., Kanda T., Abraham E., Kamba M., et al. Germline transformation of the silkworm Bombyx mori L. using a piggyBac transposon-derived vector. Nat Biotechnol. 18 (2000) 81-84
13. Nakahara Y., Kanamori Y., Kiuchi M., and Kamimura M. Effects of silkworm paralytic peptide on in vitro hematopoiesis and plasmatocyte spreading. Arch Insect Biochem Physiol 52 (2003) 163-174
14. Nakahara Y., Kanamori Y., Kiuchi M., and Kamimura M. In vitro studies of hematopoiesis in the silkworm: cell proliferation in and hemocyte discharge from the hematopoietic organ. J Insect Physiol 49 (2003) 907-916
15. Mita K., Morimyo M., Okano K., Koike Y., Nohata J., Kawasaki H., et al. The construction of an EST database for Bombyx mori and its application. Proc Natl Acad Sci U S A. 100 (2003) 14121-14126
16. Ochiai M., and Ashida M. A pattern-recognition protein for beta-1,3-glucan. The binding domain and the cDNA cloning of beta-1,3-glucan recognition protein from the silkworm, Bombyx mori. J Biol Chem 275 (2000) 4995-5002
17. Lee W.J., Lee J.D., Kravchenko V.V., Ulevitch R.J., and Brey P.T. Purification and molecular cloning of an inducible gram-negative bacteria-binding protein from the silkworm, Bombyx mori. Proc Natl Acad Sci USA 93 (1996) 7888-7893
18. Pearson A., Lux A., and Krieger M. Expression cloning of dSR-CI, a class C macrophage-specific scavenger receptor from Drosophila melanogaster. Proc Natl Acad Sci USA 92 (1995) 4056-4060
19. Hu Z.G., Chen K.P., Yao Q., Gao G.T., Xu J.P., and Chen H.Q. Cloning and characterization of Bombyx mori PP-BP a gene induced by viral infection. Yi Chuan Xue Bao 33 (2006) 975-983
20. Yu X.Q., Jiang H., Wang Y., and Kanost M.R. Nonproteolytic serine proteinase homologs are involved in prophenoloxidase activation in the tobacco hornworm, Manduca sexta. Insect Biochem Mol Biol 33 (2003) 197-208
21. Kwon T.H., Kim M.S., Choi H.W., Joo C.H., Cho M.Y., and Lee B.L. A masquerade-like serine proteinase homologue is necessary for phenoloxidase activity in the coleopteran insect, Holotrichia diomphalia larvae. Eur J Biochem 267 (2000) 6188-6196
22. Fujimoto Y., Kobayashi A., Kurata S., and Natori S. Two subunits of the insect 26/29-kDa proteinase are probably derived from a common precursor protein. J Biochem 125 (1999) 566-573
23. Jiang H., Wang Y., and Kanost M.R. Four serine proteinases expressed in Manduca sexta haemocytes. Insect Mol Biol 8 (1999) 39-53
24. Satoh D., Horii A., Ochiai M., and Ashida M. Prophenoloxidase-activating enzyme of the silkworm, Bombyx mori. Purification, characterization, and cDNA cloning. J Biol Chem 274 (1999) 7441-7453
25. Jang I.H., Chosa N., Kim S.H., Nam H.J., Lemaitre B., Ochiai M., et al. A Spatzle-processing enzyme required for toll signaling activation in Drosophila innate immunity. Dev Cell. 10 (2006) 45-55
26. Ishii K., Hamamoto H., Kamimura M., and Sekimizu K. Activation of the silkworm cytokine by bacterial and fungal cell wall components via a reactive oxygen species-triggered mechanism. J Biol Chem 283 (2008) 2185-2191
27. Matsumoto Y., Oda Y., Uryu M., and Hayakawa Y. Insect cytokine growth-blocking peptide triggers a termination system of cellular immunity by inducing its binding protein. J Biol Chem 278 (2003) 38579-38585
28. Mead G.P., Ratcliffe N.A., and Renwrantz L.R. The separation of insect haemocyte types on percoll gradients; methodology and problems. J Insect Physiol 32 (1986) 167-177
29. Pech L.L., Trudeau D., and Strand M.R. Separation and behavior in vitro of hemocytes from the moth, Pseudoplusia includens. Cell Tissue Res 277 (1994) 159-167
30. Matteucci E., and Giampietro O. Flow cytometry study of leukocyte function: analytical comparison of methods and their applicability to clinical research. Curr Med Chem 15 (2008) 596-603
31. Hillyer J.F., and Christensen B.M. Characterization of hemocytes from the yellow fever mosquito, Aedes aegypti. Histochem Cell Biol 117 (2002) 431-440
32. Nardi J.B. Embryonic origins of the two main classes of hemocytes - granular cells and plasmatocytes - in Manduca sexta. Dev Genes Evol 214 (2004) 19-28
33. Nardi J.B., Zhuang S., Pilas B., Bee C.M., and Kanost M.R. Clustering of adhesion receptors following exposure of insect blood cells to foreign surfaces. J Insect Physiol 51 (2005) 555-564
34. Gardiner E.M., and Strand M.R. Hematopoiesis in larval Pseudoplusia includens and Spodoptera frugiperda. Arch Insect Biochem Physiol 43 (2000) 147-164
35. Strand M.R. The insect cellular immune response. Insect Sci 15 (2008) 1-14
36. Ramet M., Pearson A., Manfruelli P., Li X., Koziel H., Gobel V., et al. Drosophila scavenger receptor CI is a pattern recognition receptor for bacteria. Immunity. 15 (2001) 1027-1038
37. Lavine M.D., Chen G., and Strand M.R. Immune challenge differentially affects transcript abundance of three antimicrobial peptides in hemocytes from the moth Pseudoplusia includens. Insect Biochem Mol Biol 35 (2005) 1335-1346
38. Kawabata T., Yasuhara Y., Ochiai M., Matsuura S., and Ashida M. Molecular cloning of insect pro-phenol oxidase: a copper-containing protein homologous to arthropod hemocyanin. Proc Natl Acad Sci USA 92 (1995) 7774-7778
39. Ochiai M., and Ashida M. A pattern recognition protein for peptidoglycan. Cloning the cDNA and the gene of the silkworm, Bombyx mori. J Biol Chem 274 (1999) 11854-11858
40. Yamano Y., Matsumoto M., Inoue K., Kawabata T., and Morishima I. Cloning of cDNAs for cecropins A and B, and expression of the genes in the silkworm, Bombyx mori. Biosci Biotechnol Biochem 58 (1994) 1476-1478
41. Taniai K., Kato Y., Hirochika H., and Yamakawa M. Isolation and nucleotide sequence of cecropin B cDNA clones from the silkworm, Bombyx mori. Biochim Biophys Acta 1132 (1992) 203-206
42. Yang J., Furukawa S., Sagisaka A., Ishibashi J., Taniai K., Shono T., et al. cDNA cloning and gene expression of cecropin D, an antibacterial protein in the silkworm. Bombyx mori. Comp Biochem Physiol B Biochem Mol Biol. 122 (1999) 409-414
43. Chowdhury S., Taniai K., Hara S., Kadono-Okuda K., Kato Y., Yamamoto M., et al. cDNA cloning and gene expression of lebocin, a novel member of antibacterial peptides from the silkworm. Bombyx mori. Biochem Biophys Res Commun. 214 (1995) 271-278
44. Kamimura M., Nakahara Y., Kanamori Y., Tsuzuki S., Hayakawa Y., and Kiuchi M. Molecular cloning of silkworm paralytic peptide and its developmental regulation. Biochem Biophys Res Commun 286 (2001) 67-73
45. Xu Y., and Kawasaki H. Isolation and expression of cathepsin B cDNA in hemocytes during metamorphosis of Bombyx mori. Comp Biochem Physiol B: Biochem Mol Biol 130 (2001) 393-399
46. Yamamoto Y., Takimoto K., Izumi S., Toriyama-Sakurai M., Kageyama T., and Takahashi S.Y. Molecular cloning and sequencing of cDNA that encodes cysteine proteinase in the eggs of the silkmoth, Bombyx mori. J Biochem 116 (1994) 1330-1335
47. Gui Z.Z., Lee K.S., Kim B.Y., Choi Y.S., Wei Y.D., Choo Y.M., et al. Functional role of aspartic proteinase cathepsin D in insect metamorphosis. BMC Dev Biol. 6 (2006) 49
48. Matsuoka T., and Fujiwara H. Expression of ecdysteroid-regulated genes is reduced specifically in the wing discs of the wing-deficient mutant (fl) of Bombyx mori. Dev Genes Evol 210 (2000) 120-128
49. Koumarianou P., Marcos A.G., Ballesta J.P., and Kouyanou-Koutsoukou S. In vivo analysis of the acidic ribosomal proteins BmP1 and BmP2 of the silkworm Bombyx mori in the yeast Saccharomyces cerevisiae. Gene 388 (2007) 27-33