In vivo analysis of the acidic ribosomal proteins BmP1 and BmP2 of the silkworm Bombyx mori in the yeast Saccharomyces cerevisiae

Gene

Volumn 388, Issue 1-2, 2007, Pages 27-33

  Koumarianou, Petrina ^a   Marcos, Alberto Garcia ^b   Ballesta, Juan P G ^b   Kouyanou Koutsoukou, Sophia ^a  

^a UNIVERSITY OF ATHENS   (Greece)

^b UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

Author keywords

Complementation; Conditional mutants; P proteins; Phosphorylation; Ribosomal stalk

Indexed keywords

BONE MORPHOGENETIC PROTEIN 2; COMPLEMENTARY DNA; P1 PROTEIN; P1ALPHA PROTEIN; P1BETA PROTEIN; P2BETA PROTEIN; PROCOLLAGEN C PROTEINASE; PROTEIN; PROTEIN P2; RIBOSOME PROTEIN; UNCLASSIFIED DRUG;

ACIDITY; ARTICLE; CONTROLLED STUDY; CYTOPLASM; FUNGAL STRAIN; IN VIVO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; RIBOSOME; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY; SILKWORM; YEAST;

AMINO ACID SEQUENCE; ANIMALS; BOMBYX; FEMALE; GENETIC COMPLEMENTATION TEST; IMMUNOBLOTTING; INSECT PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; OVARIAN FOLLICLE; PLASMIDS; RECOMBINANT PROTEINS; RIBOSOMAL PROTEINS; RIBOSOMES; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY, AMINO ACID; TRANSFORMATION, GENETIC;

BOMBYX MORI; SACCHAROMYCES CEREVISIAE;

EID: 33845751012     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.gene.2006.09.017     Document Type: Article

References (37)

1. Ballesta J.P.G., and Remacha M. The large ribosomal subunit stalk as a regulatory element of the eukaryotic translational machinery. Prog. Nucleic Acid Res. Mol. Biol. 55 (1996) 157-193
2. Diaconu M., et al. Structural basis for the function of the ribosomal L7/12 stalk in factor binding and GTPase activation. Cell 121 (2005) 991-1004
3. Gagou M.E., Rodriguez-Gabriel M.A., Ballesta J.P.G., and Kouyanou S. The ribosomal P-proteins of the medfly Ceratitis capitata form a heterologous stalk structure interacting with the endogenous P-proteins, in conditional P0-null strains of the yeast Saccharomyces cerevisiae. Nucleic Acids Res. 28 3 (2000) 736-743
4. Gonzalo P., Lavergne J.P., and Reboud J.P. Pivotal role of the P1 N-terminal domain in the assembly of the mammalian ribosomal stalk and in the proteosynthetic activity. J. Biol. Chem. 276 (2001) 19762-19769
5. Gonzalo P., and Reboud J.P. The puzzling lateral flexible stalk of the ribosome. Biol. Cell. 95 (2003) 179-193
6. Guarinos E., Remacha M., and Ballesta J.P.G. Asymmetric interactions between the acidic P1 and P2 proteins in the Saccharomyces cerevisiae ribosomal stalk. J. Biol. Chem. 276 (2001) 32474-32479
7. Guarinos E., Santos C., Sanchez A., Qiu D.-Y., Remacha M., and Ballesta J.P. Tag-mediated fractionation of yeast ribosome populations proves the monomeric organization of the eukaryotic ribosomal stalk structure. Mol. Microbiol. 50 (2003) 703-712
8. Hagiya A., et al. A mode of assembly of P0, P1, and P2 proteins at the GTPase-associated center in animal ribosome: in vitro analyses with P0 truncation mutants. J. Biol. Chem. 280 (2005) 39193-39199
9. Hasler P., Brot N., Weisbach H., Parnassa A.P., and Elkon K.B. Ribosomal proteins P0, P1, and P2 are phosphorylated by casein kinase II at their conserved carboxyl termini. J. Biol. Chem. 266 (1991) 13815-13820
10. Ilag L.L., et al. Heptameric (L12)6/L10 rather than canonical pentameric complexes are found by tandem MS of intact ribosomes from thermophilic bacteria. Proc. Natl. Acad. Sci. U. S. A. 102 (2005) 8192-8197
11. Kouyanou S., Santos C., Koliaraki V., and Ballesta J.P.G. Protein BmP0 from the silkworm Bombyx mori can be assembled and is functional in the Saccharomyces cerevisiae ribosomal stalk in the absence of the acidic P1 and P2 proteins. Gene 314 (2003) 173-179
12. Krokowski D., Tchorzewski M., Boguszewska A., and Grankowski N. Acquisition of a stable structure by yeast ribosomal P0 protein requires binding of P1A-P2B complex: in vitro formation of the stalk structure. Biochim. Biophys Acta 1724 (2005) 59-70
13. Krokowski D., Boguszewska A., Abramczyk D., Liljas A., Tchorzewski M., and Grankowski N. Yeast ribosomal P0 protein has two separate binding sites for P1/P2 proteins. Mol. Microbiol. 60 (2006) 386-400
14. Lalioti V.S., Perez-Fernández J., Remacha M., and Ballesta J.P.G. Characterization of interaction sites in the Saccharomyces cerevisiae ribosomal stalk components. Mol. Microbiol. 46 (2002) 719-729
15. Mita K., Morimoyo M., Okano K., Shimada T., and Maeda S. The construction of EST database for genome analysis of Bombyx mori. Riken Rev. 22 (1999) 63-67
16. Nusspaumer G., Remacha M., and Ballesta J.P. Phosphorylation and N-end region of the yeast ribosomal protein P1 mediate its degradation, which is prevented by protein P2. EMBO J. 19 (2000) 6075-6084
17. Payo J.M., Santana-Roman H., Remacha M., Ballesta J.P.G., and Zinker S. The eukaryotic phosphoproteins interact with the ribosome through their amino terminal domain. Biochemistry 34 (1995) 7841-7948
18. Pettersson I., Hardy S.J., and Liljas A. The ribosomal protein L8 is a complex of L7/L12 and L10. FEBS Lett. 64 (1976) 135-138
19. Pérez-Fernández J., Remacha M., and Ballesta J.P.G. The acidic protein binding site is partially hidden in the free S. cerevisiae ribosomal stalk protein P0. Biochemistry 44 (2005) 5532-5540
20. Pustell J., and Kafatos F.C. A convenient and adaptable package of computer programs for DNA and protein sequence management, analysis and homology determination. Nucleic Acids Res. 12 (1984) 643-655
21. Remacha M., Santos C., Bermecho B., Naranda T., and Ballesta J.P.G. Stable binding of the eukaryotic acidic phosphoproteins to the ribosomes is not an absolute requirement for in vivo protein synthesis. J. Biol. Chem. 267 (1992) 12061-12067
22. Remacha M., Jimenez-Diaz A., Bermejo B., Rodriguez-Gabriel M.A., Guarinos E., and Ballesta J.P.G. Ribosomal acidic phosphoproteins P1 and P2 are not required for cell viability but regulate the pattern of protein expression in Saccharomyces cerevisiae. Mol. Cell. Biol. 15 (1995) 4754-4762
23. Rodriguez-Gabriel M.A., Remacha M., and Ballesta J.P.G. Phosphorylation of ribosomal protein P0 is not essential for ribosome function but can affect translation. Biochemistry 37 (1998) 16620-16626
24. Rodriguez-Gabriel M.A., Remacha M., and Ballesta J.P.G. The RNA interacting domain but not the protein interacting domain is highly conserved in ribosomal P0. J. Biol. Chem. 275 (2000) 2130-2136
25. Santos C., and Ballesta J.P.G. Ribosomal phosphoprotein P0, contrary to phosphoproteins P1 and P2, is required for S. cerevisiae viability and ribosome assembly. JBC 269 (1994) 15689-15696
26. Santos C., and Ballesta J.P.G. Role of the ribosomal stalk components in the resistance of Aspergillus fumigatus to the sordarin antifungals. Mol. Microbiol. 43 1 (2002) 227-237
27. Shimizu T., Nakagaki M., Nishi Y., Kobayashi Y., Hachimori A., and Uchiumi T. Interaction among silkworm ribosomal proteins P1, P2 and P0 required for functional protein binding to the GTPase-associated domain of 28S rRNA. Nucleic Acids Res. 30 (2002) 2620-2627
28. Shimmin L.C., Ramirez G., Matheson A.T., and Dennis P.P. Sequence alignment and evolutionary comparison of the L10 equivalent and L12 equivalent ribosomal proteins from archaebacterial, eubacterial and eukaryotes. J. Mol. Evol. 29 (1989) 448-462
29. Subramanian A.R. Copies of proteins L7 and L12 and heterogeneity of the large subunit of Escherichia coli ribosome. J. Mol. Biol. 95 (1975) 1-8
30. Tchorzewski M., Krokowski D., Boguszewska A., Liljas A., and Grankowski N. Structural characterization of yeast acidic ribosomal P proteins forming the P1A-P2B heterocomplex. Biochemistry 42 (2003) 3399-3408
31. Tsurugi K., and Ogata K. Evidence for the exchangeability of acidic ribosomal proteins on cytoplasmic ribosomes in regenerating rat liver. J. Biochem. 98 (1985) 1427-1431
32. Vard C., Guillot D., Bargis P., Lavergne J.P., and Reboud J.P. A specific role for the phosphorylation of mammalian acidic ribosomal protein P2. J. Biol. Chem. 272 (1997) 20259-20262
33. Vilella M.D., Remacha M., Ortiz B.L., Mendez E., and Ballesta J.P.G. Characterization of the yeast acidic ribosomal phosphoproteins using monoclonal antibodies. Proteins L44/L45 and L44V have different functional roles. Eur. J. Biochem. 196 (1991) 407-414
34. Wahl M.C., and Möller W. Structure and function of the acidic ribosomal stalk proteins. Curr. Protein Pept. Sci. 3 (2002) 93-106
35. Zambrano R., Briones E., Remacha M., and Ballesta J.P.G. Phosphorylation of the acidic ribosomal P proteins in Saccharomyces cerevisiae. A reappraisal. Biochemistry 36 (1997) 14439-14446
36. Zinker S., and Warner J.R. The ribosomal proteins of Saccharomyces cerevisiae: phosphorylated and exchangeable proteins. J. Biol. Chem. 251 (1976) 1799-1807
37. Zurdo J., Parada P., van den Berg A., Nusspaumer G., Jimenez-Diaz A., Remacha M., and Ballesta J.P. Assembly of Saccharomyces cerevisiae ribosomal stalk: binding of P1 proteins is required for the interaction of P2 proteins. Biochemistry 39 (2000) 8929-8934