Heterologous expression of polyhydroxyalkanoate depolymerase from Thermobifida sp. in Pichia pastoris and catalytic analysis by surface plasmon resonance

Applied Microbiology and Biotechnology

Volumn 82, Issue 1, 2009, Pages 131-140

  Phithakrotchanakoon, Chitwadee ^a   Daduang, Ratama ^b   Thamchaipenet, Arinthip ^b   Wangkam, Thidarat ^c   Srikhirin, Toemsak ^c   Eurwilaichitr, Lily ^a   Champreda, Verawat ^a  

^a NATIONAL SCIENCE AND TECHNOLOGY DEVELOPMENT AGENCY   (Thailand)

^b KASETSART UNIVERSITY   (Thailand)

^c MAHIDOL UNIVERSITY   (Thailand)

Author keywords

Atomic force microscopy; Pichia pastoris; Polyhydroxyalkanoate; Polyhydroxyalkanoate depolymerase; Surface plasmon resonance; Thermofida sp.

Indexed keywords

3-HYDROXYBUTYRATE (PHB); 3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE (PHBHV); DEGRADATION RATES; DEPOLYMERASE; ESTERASE ACTIVITIES; FORCE MICROSCOPY; HETEROLOGOUS EXPRESSIONS; P. PASTORIS; PICHIA PASTORIS; POLYHYDROXYALKANOATE; POLYHYDROXYALKANOATE DEPOLYMERASE; PRIMARY STRUCTURE ANALYSIS; SURFACE DETERIORATION; SURFACE PLASMON RESONANCE (SPR); THERMOFIDA SP.;

AMINO ACIDS; AMORPHOUS FILMS; ATOMIC FORCE MICROSCOPY; ATOMIC PHYSICS; ATOMS; DEGRADATION; ENZYME ACTIVITY; ENZYMES; PLASMONS; POLYMERS; REINFORCED PLASTICS; RESONANCE; SURFACE PLASMON RESONANCE;

SURFACE ANALYSIS;

BACTERIUM; CATALYSIS; ENZYME ACTIVITY; GENE EXPRESSION; YEAST;

ACTINOMYCETALES; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CARBOXYLIC ESTER HYDROLASES; CATALYSIS; CLONING, MOLECULAR; GENE EXPRESSION; MOLECULAR SEQUENCE DATA; PICHIA; SEQUENCE ALIGNMENT; SURFACE PLASMON RESONANCE;

PICHIA PASTORIS; THERMOBIFIDA;

EID: 58549091878     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1754-1     Document Type: Article

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