Identification and expression of cellobiohydrolase (CBHI) gene from an endophytic fungus, Fusicoccum sp. (BCC4124) in Pichia pastoris

Protein Expression and Purification

Volumn 58, Issue 1, 2008, Pages 148-153

  Kanokratana, Pattanop ^a   Chantasingh, Duriya ^a   Champreda, Verawat ^a   Tanapongpipat, Sutipa ^a   Pootanakit, Kusol ^b   Eurwilaichitr, Lily ^a  

^a NATIONAL SCIENCE AND TECHNOLOGY DEVELOPMENT AGENCY   (Thailand)

^b MAHIDOL UNIVERSITY   (Thailand)

Author keywords

Cellobiohydrolase; Cellulose binding domain; Endophytic fungus; Fusicoccum; Pichia pastoris

Indexed keywords

CELLULOSE 1,4 BETA CELLOBIOSIDASE; RECOMBINANT PROTEIN;

ARTICLE; ASCOMYCETES; ENZYMOLOGY; FUNGAL GENE; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PICHIA;

ASCOMYCOTA; CELLULOSE 1,4-BETA-CELLOBIOSIDASE; CLONING, MOLECULAR; GENE EXPRESSION; GENES, FUNGAL; PICHIA; RECOMBINANT PROTEINS;

ASCOMYCOTA; FUNGI; FUSICOCCUM; PICHIA PASTORIS;

EID: 38549176702     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.09.008     Document Type: Article

References (19)

1. Bhat M.K. Cellulases and related enzymes in biotechnology. Biotechnol. Adv. 18 (2000) 355-383
2. Teeri T.T. Crystalline cellulose degradation: new insight into the function of cellobiohydrolases. Trends Biotechnol. 15 (1997) 160-167
3. Takashima S., Iikura H., Nakamura A., Hidaka M., Masaki H., and Uozumi T. Isolation of the gene and characterization of the enzymatic properties of a major exoglucanase of Humicola grisea without a cellulose-binding domain. J. Biochem. 124 (1998) 717-725
4. Henrissat B. A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 280 (1991) 309-316
5. Hong J., Tamaki H., Yamamoto K., and Kumagai H. Cloning of a gene encoding thermostable cellobiohydrolase from Thermoascus aurantiacus and its expression in yeast. Appl. Microbiol. Biotechnol. 63 (2003) 42-50
6. Haakana H., Miettinen-Oinonen A., Joutsjoki V., Mantyla A., Suominen P., and Vehmaanpera J. Cloning of cellulase genes from Melanocarpus albomyces and their efficient expression in Tricoderma reesei. Enzyme Microb. Technol. 34 (2004) 159-167
7. Lee C.C., Wong D.W., and Robertson G.H. Cloning and characterization of two cellulase genes from Lentinula edodes. FEMS Microbiol. Lett. 205 (2001) 355-360
8. Jia J., Dyer P.S., Buswell J.A., and Peberdy J.F. Cloning of the cbhI and cbhII genes involved in cellulose utilisation by the straw mushroom Volvariella volvacea. Mol. Gen. Genet. 261 (1999) 985-993
9. Gilkes N., Henrissat B., Kilburn D., Miller R., and Warren R. Domains in microbial β-1,4-glycanases sequence conservation, function, and enzyme families. Microbiol. Rev. 55 (1991) 303-315
10. Van Tilbeurgh H., Tomme P., Claeyssens M., Bhikhabhai R., and Pettersson G. Limited proteolysis of the cellobiohydrolase I from Trichoderma reesei. Separation of the functional domains. FEBS Lett. 204 (1986) 223-227
11. Frohman M., Dush M., and Martin G. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85 (1988) 8998-9002
12. Ghose T.K. Measurement of cellulase activities. Pure Appl. Chem. 59 (1987) 257-268
13. Miller G.L. Using dinitrosalicylic acid for determination of reducing sugar. Anal. Chem. 31 (1959) 426-428
14. Bendtsen J.D., Nielsen H., von Heijne G., and Brunak S. Improved prediction of signal peptides: SignalP 3.0. J. Mol. Biol. 340 (2004) 783-795
15. Dawson R., Elliott D., Elliott W., and Jones K. Data for Biochemical Research. third ed. (1986), Clarendon Press, Oxford
16. Stahlberg J., Divne C., Koivula A., Piens K., Claeyssens M., Teeri T.T., and Jones T.A. Activity studies and crystal structures of catalytically deficient mutants of cellobiohydrolase I from Trichoderma reesei. J. Mol. Biol. 264 (1996) 337-349
17. Gusakov A.V., Sinitsyn A.P., Salanovich T.N., Bukhtojarov F.E., Markov A.V., Ustinov B.B., van Zeijl C., Punt P., and Burlingame R. Purification, cloning and characterisation of two forms of thermostable and highly active cellobiohydrolase I (Cel7A) produced by the industrial strain of Chrysosporium lucknowense. Enzyme Microb. Technol. 36 (2005) 57-69
18. Lahjouji K., Storms R., Xiao Z., Joung K.B., Zheng Y., Powlowski J., Tsang A., and Varin L. Biochemical and molecular characterization of a cellobiohydrolase from Trametes versicolor. Appl. Microb. Biotechnol. 75 (2007) 337-346
19. Asghari S.M., Khajeh K., Moradian F., Ranjbar B., and Naderi-Manesh H. Acid-induced conformational changes in Bacillus amyloliquefaciens alpha-amylase: appearance of a molten globule like state. Enzyme Microb. Technol. 35 (2004) 51-57