Cytotoxic necrotizing factor type 1-neutralizing monoclonal antibody NG8 recognizes three amino acids in a C-terminal region of the toxin and reduces toxin binding to HEp-2 cells

Infection and Immunity

Volumn 77, Issue 1, 2009, Pages 170-179

  Grande, Kerian K ^a   Meysick, Karen C ^b   Rasmussen, Susan B ^a   O'Brien, Alison D ^a  

^a UNIFORMED SERVICES UNIVERSITY OF THE HEALTH SCIENCES   (United States)

^b CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOTOXIC NECROTIZING FACTOR 1; CYTOTOXIC NECROTIZING FACTOR 2; MONOCLONAL ANTIBODY NG8; NEUTRALIZING ANTIBODY; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CHIMERA; CONTROLLED STUDY; DEAMINATION; ESCHERICHIA COLI; HUMAN; HUMAN CELL; IN VITRO STUDY; MULTINUCLEAR CELL; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; SITE DIRECTED MUTAGENESIS;

AMINO ACID SUBSTITUTION; ANTIBODIES, BACTERIAL; ANTIBODIES, MONOCLONAL; BACTERIAL TOXINS; CELL LINE; EPITOPE MAPPING; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HEPATOCYTES; HUMANS; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING;

EID: 58449083623     PISSN: 00199567     EISSN: 10985522     Source Type: Journal    
DOI: 10.1128/IAI.00943-08     Document Type: Article

References (34)

1. Aktories, K. 1997. Rho proteins: targets for bacterial toxins. Trends Microbiol. 5:282-288.
2. Aktories, K., G. Schmidt, and I. Just. 2000. Rho GTPases as targets of bacterial protein toxins. Biol. Chem. 381:421-426.
3. Barbieri, J. T., M. J. Riese, and K. Aktories. 2002. Bacterial toxins that modify the actin cytoskeleton. Annu. Rev. Cell Dev. Biol. 18:315-344.
4. Blanco, J., M. Blanco, E. A. Gonzalez, M. P. Alonso, and J. I. Garabal. 1990. Comparative evaluation of three tests for the detection of Escherichia coli cytotoxic necrotizing factors (CNF1 and CNF2) using filtrates of cultures treated with mitomycin C. FEMS Microbiol. Lett. 57:311-316.
5. Blumenthal, B., C. Hoffmann, K. Aktories, S. Backert, and G. Schmidt. 2007. The cytotoxic necrotizing factors from Yersinia pseudotuberculosis and from Escherichia coli bind to different cellular receptors but take the same route to the cytosol. Infect. Immun. 75:3344-3353.
6. Boquet, P., and E. Lemichez. 2003. Bacterial virulence factors targeting Rho GTPases: parasitism or symbiosis? Trends Cell Biol. 13:238-246.
7. Buetow, L., G. Flatau, K. Chiu, P. Boquet, and P. Ghosh. 2001. Structure of the Rho-activating domain of Escherichia coli cytotoxic necrotizing factor 1. Nat. Struct. Biol. 8:584-588.
8. Buetow, L., and P. Ghosh. 2003. Structural elements required for deamidation of RhoA by cytotoxic necrotizing factor 1. Biochemistry 42:12784-12791.
9. Caprioli, A., V. Falbo, L. G. Roda, F. M. Ruggeri, and C. Zona. 1983. Partial purification and characterization of an Escherichia coli toxic factor that induces morphological cell alterations. Infect. Immun. 39:1300-1306.
10. Chung, J. W., S. J. Hong, K. J. Kim, D. Goti, M. F. Stins, S. Shin, V. L. Dawson, T. M. Dawson, and K. S. Kim. 2003. 37-kDa laminin receptor precursor modulates cytotoxic necrotizing factor 1-mediated RhoA activation and bacterial uptake. J. Biol. Chem. 278:16857-16862.
11. Davis, J. M., S. B. Rasmussen, and A. D. O'Brien. 2005. Cytotoxic necrotizing factor type 1 production by uropathogenic Escherichia coli modulates polymorphonuclear leukocyte function. Infect. Immun. 73:5301-5310.
12. De Rycke, J., E. A. González, J. Blanco, E. Oswald, M. Blanco, and R. Boivin. 1990. Evidence for two types of cytotoxic necrotizing factor in human and animal clinical isolates of Escherichia coli. J. Clin. Microbiol. 28:694-699.
13. De Rycke, J., J. F. Guillot, and R. Boivin. 1987. Cytotoxins in non-enterotoxigenic strains of Escherichia coli isolated from feces of diarrheic calves. Vet. Microbiol. 15:137-150.
14. Donelli, G., and C. Fiorentini. 1992. Cell injury and death caused by bacterial protein toxins. Toxicol. Lett. 64-65(Spec. No.):695-699.
15. Fiorentini, C., G. Arancia, A. Caprioli, V. Falbo, F. M. Ruggeri, and G. Donelli. 1988. Cytoskeletal changes induced in HEp-2 cells by the cytotoxic necrotizing factor of Escherichia coli. Toxicon 26:1047-1056.
16. Flatau, G., E. Lemichez, M. Gauthier, P. Chardin, S. Paris, C. Fiorentini, and P. Boquet. 1997. Toxin-induced activation of the G protein p21 Rho by deamidation of glutamine. Nature 387:729-733.
17. Frankel, G., D. C. Candy, P. Everest, and G. Dougan. 1994. Characterization of the C-terminal domains of intimin-like proteins of enteropathogenic and enterohemorrhagic Escherichia coli, Citrobacter freundii, and Hafnia alvei. Infect. Immun. 62:1835-1842.
18. Hoffmann, C., K. Aktories, and G. Schmidt. 2007. Change in substrate specificity of cytotoxic necrotizing factor unmasks proteasome-independent down-regulation of constitutively active RhoA. J. Biol. Chem. 282:10826-10832.
19. Horiguchi, Y. 2001. Bordetella dermonecrotizing toxin and Escherichia coli cytotoxic necrotizing factors: bacterial toxins activating Rho family GTPases. Tanpakushitsu Kakusan Koso 46:491-496. (In Japanese.)
20. Lerm, M., J. Selzer, A. Hoffmeyer, U. R. Rapp, K. Aktories, and G. Schmidt. 1999. Deamidation of Cdc42 and Rac by Escherichia coli cytotoxic necrotizing factor 1: activation of c-Jun N-terminal kinase in HeLa cells. Infect. Immun. 67:496-503.
21. Lockman, H. A., R. A. Gillespie, B. D. Baker, and E. Shakhnovich. 2002. Yersinia pseudotuberculosis produces a cytotoxic necrotizing factor. Infect. Immun. 70:2708-2714.
22. McNichol, B. A., S. B. Rasmussen, H. M. Carvalho, K. C. Meysick, and A. D. O'Brien. 2007. Two domains of cytotoxic necrotizing factor type 1 bind the cellular receptor, laminin receptor precursor protein. Infect. Immun. 75:5095-5104.
23. McNichol, B. A., S. B. Rasmussen, K. C. Meysick, and A. D. O'Brien. 2006. A single amino acid substitution in the enzymatic domain of cytotoxic necrotizing factor type 1 of Escherichia coli alters the tissue culture phenotype to that of the dermonecrotic toxin of Bordetella spp. Mol. Microbiol. 60:939-950.
24. Meysick, K. C., M. Mills, and A. D. O'Brien. 2001. Epitope mapping of monoclonal antibodies capable of neutralizing cytotoxic necrotizing factor type 1 of uropathogenic Escherichia coli. Infect. Immun. 69:2066-2074.
25. Mills, M., K. C. Meysick, and A. D. O'Brien. 2000. Cytotoxic necrotizing factor type 1 of uropathogenic Escherichia coli kills cultured human uroepithelial 5637 cells by an apoptotic mechanism. Infect. Immun. 68:5869-5880.
26. Munro, P., and E. Lemichez. 2005. Bacterial toxins activating Rho GTPases. Curr. Top. Microbiol. Immunol. 291:177-190.
27. Orden, J. A., G. Domínguez-Bernal, S. Martínez- Pulgarín, M. Blanco, J. E. Blanco, A. Mora, J. Blanco, J. Blanco, and R. de la Fuente. 2007. Necrotoxigenic Escherichia coli from sheep and goats produce a new type of cytotoxic necrotizing factor (CNF3) associated with the eae and ehxA genes. Int. Microbiol. 10:47-55.
28. Rippere-Lampe, K. E., M. Lang, H. Ceri, M. Olson, H. A. Lockman, and A. D. O'Brien. 2001. Cytotoxic necrotizing factor type 1-positive Escherichia coli causes increased inflammation and tissue damage to the prostate in a rat prostatitis model. Infect. Immun. 69:6515-6519.
29. Rippere-Lampe, K. E., A. D. O'Brien, R. Conran, and H. A. Lockman. 2001. Mutation of the gene encoding cytotoxic necrotizing factor type 1 (cnf1) attenuates the virulence of uropathogenic Escherichia coli. Infect. Immun. 69:3954-3964.
30. Schmidt, G., P. Sehr, M. Wilm, J. Selzer, M. Mann, and K. Aktories. 1997. Gln 63 of Rho is deamidated by Escherichia coli cytotoxic necrotizing factor-1. Nature 387:725-729.
31. Schmidt, G., J. Selzer, M. Lerm, and K. Aktories. 1998. The Rho-deamidating cytotoxic necrotizing factor 1 from Escherichia coli possesses transglutaminase activity. Cysteine 866 and histidine 881 are essential for enzyme activity. J. Biol. Chem. 273:13669-13674.
32. Self, A. J., and A. Hall. 1995. Purification of recombinant Rho/Rac/G25K from Escherichia coli. Methods Enzymol. 256:3-10.
33. Smith, Y. C., S. B. Rasmussen, K. K. Grande, R. M. Conran, and A. D. O'Brien. 2008. Hemolysin of uropathogenic Escherichia coli evokes extensive shedding of the uroepithelium and hemorrhage in bladder tissue within the first 24 hours after intraurethral inoculation of mice. Infect. Immun. 76:2978-2990.
34. Sugai, M., K. Hatazaki, A. Mogami, H. Ohta, S. Y. Pérès, F. Herault, Y. Horiguchi, M. Masuda, Y. Ueno, H. Komatsuzawa, H. Suginaka, and E. Oswald. 1999. Cytotoxic necrotizing factor type 2 produced by pathogenic Escherichia coli deamidates a Gln residue in the conserved G-3 domain of the Rho family and preferentially inhibits the GTPase activity of RhoA and Rac1. Infect. Immun. 67:6550-6557.