EPR spin-trapping and nano LC MS/MS techniques for DEPMPO/ •OOH and immunospin-trapping with anti-DMPO antibody in mitochondrial electron transfer system

Methods in Molecular Biology

Volumn 477, Issue , 2008, Pages 75-88

  Chen, Yeong Renn ^a  

^a THE OHIO STATE UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

Electron transport chain; EPR spin trapping; Immunospin trapping; Mitochondria; Protein radical; Superoxide

Indexed keywords

(DIETHOXYPHOSPHORYL) 5 METHYLPYRROLINE N OXIDE; (DIETHOXYPHOSPHORYL)-5-METHYLPYRROLINE N-OXIDE; AMINE OXIDE; ANTIBODY; FREE RADICAL; PEPTIDE; PYRROLE DERIVATIVE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE; SUCCINATE DEHYDROGENASE (UBIQUINONE); SUPEROXIDE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BINDING SITE; CATTLE; CHEMISTRY; ELECTRON SPIN RESONANCE; ELECTRON TRANSPORT; ENZYMOLOGY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MITOCHONDRION; MOLECULAR GENETICS; NANOTECHNOLOGY; OXIDATIVE STRESS; SPIN TRAPPING;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES; BINDING SITES; CATTLE; CHROMATOGRAPHY, LIQUID; CYCLIC N-OXIDES; ELECTRON SPIN RESONANCE SPECTROSCOPY; ELECTRON TRANSPORT; ELECTRON TRANSPORT COMPLEX II; FREE RADICALS; MASS SPECTROMETRY; MITOCHONDRIA; MOLECULAR SEQUENCE DATA; NADH DEHYDROGENASE; NANOTECHNOLOGY; OXIDATIVE STRESS; PEPTIDES; PYRROLES; SPIN TRAPPING; SUPEROXIDES;

EID: 58149397229     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-517-0_7     Document Type: Article

References (15)

1. Chen, Y. R., Chen, C. L., Zhang, L., Green-Church, K. B., and Zweier, J. L. (2005) Superoxide generation from mitochondrial NADH dehydrogenase induces self-inactivation with specific protein radical formation. J Biol Chem 280, 37339-37348.
2. Chen, Y. R., Chen, C. L., Yeh, A., Liu, X., and Zweier, J. L. (2006) Direct and indirect roles of cytochrome b in the mediation of superoxide generation and NO catabolism by mitochondrial succinate-cytochrome c reductase. J Biol Chem 281, 13159-13168.
3. Chen, Y. R., Chen, C. L., Pfeiffer, D. R., and Zweier, J. L. (2007) Mitochondrial complex II in post-ischemic heart: Oxidative injury and the role of protein Sglutathionylation. J Biol Chem 282, 32640-32654.
4. Guo, J., and Lemire, B. D. (2003) The ubiquinone-binding site of the Saccharomyces cerevisiae succinateubiquinone oxidoreductase is a source of superoxide. J Biol Chem 278, 47629-47635.
5. Messner, K. R., and Imlay, J. A. (2002) Mechanism of superoxide and hydrogen peroxide formation by fumarate reductase, succinate dehydrogenase, and aspartate oxidase. J Biol Chem 277, 42563-42571.
6. Vasquez-Vivar, J., Kalyanaraman, B., Martasek, P., Hogg, N., Masters, B. S., Karoui, H., Tordo, P., and Pritchard, K. A., Jr. (1998) Superoxide generation by endothelial nitric oxide synthase: the influence of cofactors. Proc Natl Acad Sci U S A 95, 9220-9225.
7. Roubaud, V., Sankarapandi, S., Kuppusamy, P., Tordo, P., and Zweier, J. L. (1997) Quantitative measurement of superoxide generation using the spin trap 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide. Anal Biochem 247, 404-411.
8. Frejaville, C., Karoui, H., Tuccio, B., Le Moigne, F., Culcasi, M., Pietri, S., Lauricella, R., and Tordo, P. (1995) 5-(Diethoxyphosphoryl)-5- methyl-1-pyrroline N-oxide: a new efficient phosphorylated nitrone for the in vitro and in vivo spin trapping of oxygen-centered radicals. J Med Chem 38, 258-265.
9. Detweiler, C. D., Deterding, L. J., Tomer, K. B., Chignell, C. F., Germolec, D., and Mason, R. P. (2002) Immunological identification of the heart myoglobin radical formed by hydrogen peroxide. Free Radic Biol Med 33, 364-369.
10. Chen, C. L., Zhang, L., Yeh, A., Chen, C. A., Green-Church, K. B., Zweier, J. L., and Chen, Y. R. (2007) Site-specific S-glutathiolation of mitochondrial NADH ubiquinone reductase. Biochemistry 46, 5754-5765.
11. Boveris, A. (1984) Determination of the production of superoxide radicals and hydrogen peroxide in mitochondria. Methods Enzymol 105, 429-435.
12. Taylor, E. R., Hurrell, F., Shannon, R. J., Lin, T. K., Hirst, J., and Murphy, M. P. (2003) Reversible glutathionylation of complex I increases mitochondrial superoxide formation. J Biol Chem 278, 19603-19610.
13. Deterding, L. J., Ramirez, D. C., Dubin, J. R., Mason, R. P., and Tomer, K. B. (2004) Identification of free radicals on hemoglobin from its self-peroxidation using mass spectrometry and immuno-spin trapping: observation of a histidinyl radical. J Biol Chem 279, 11600-11607.
14. Chen, Y. R., Chen, C. L., Liu, X., Li, H., Zweier, J. L., and Mason, R. P. (2004) Involvement of protein radical, protein aggregation, and effects on NO metabolism in the hypochlorite-mediated oxidation of mitochondrial cytochrome c. Free Radic Biol Med 37, 1591-1603.
15. Duling, D. R. (1994) Simulation of multiple isotropic spin-trap EPR spectra. J Magn Reson B 104, 105-110.