Immunological identification of the heart myoglobin radical formed by hydrogen peroxide

Free Radical Biology and Medicine

Volumn 33, Issue 3, 2002, Pages 364-369

  Detweiler, Charles D ^a   Deterding, Leesa J ^a   Tomer, Kenneth B ^a   Chignell, Colin F ^a   Germolec, Dori ^a   Mason, Ronald P ^a  

^a NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES   (United States)

Author keywords

5,5 dimethyl 1 pyrroline N oxide (DMPO); Antibodies; Free radicals; Myoglobin; Protein radical; Western blot

Indexed keywords

FREE RADICAL; HYDROGEN PEROXIDE; MYOGLOBIN; NITRONE; PHTHALIC ACID DIMETHYL ESTER; POLYCLONAL ANTIBODY; 5,5 DIMETHYL 1 PYRROLINE 1 OXIDE; 5,5-DIMETHYL-1-PYRROLINE-1-OXIDE; AMINE OXIDE; METMYOGLOBIN; NITROGEN OXIDE; NITRONES;

ANTIBODY SPECIFICITY; ANTIGEN BINDING; ARTICLE; PEROXIDATION; PRIORITY JOURNAL; SPIN TRAPPING; SUPERNATANT; WESTERN BLOTTING; ANIMAL; ANTIBODY PRODUCTION; CHEMISTRY; DRUG EFFECT; ELECTRON SPIN RESONANCE; ENZYME LINKED IMMUNOSORBENT ASSAY; HEART; HEART MUSCLE; HORSE; MASS SPECTROMETRY; METABOLISM; OXIDATION REDUCTION REACTION; RABBIT; RAT; SPIN LABELING;

ANIMAL; ANTIBODY FORMATION; BLOTTING, WESTERN; CYCLIC N-OXIDES; ELECTRON SPIN RESONANCE SPECTROSCOPY; ENZYME-LINKED IMMUNOSORBENT ASSAY; FREE RADICALS; HEART; HORSES; HYDROGEN PEROXIDE; METMYOGLOBIN; MYOCARDIUM; MYOGLOBIN; NITROGEN OXIDES; OXIDATION-REDUCTION; RABBITS; RATS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SPIN LABELS; ANIMALS;

EQUUS CABALLUS;

EID: 0036667549     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0891-5849(02)00895-X     Document Type: Article

References (21)

1. Spin trapping
2. Two decades of spin trapping
3. In vivo spin trapping: From chemistry to toxicology
4. Generation and propagation of radical reactions on proteins
5. ESR spin-trapping of a protein-derived tyrosyl radical from the reaction of cytochrome c with hydrogen peroxide
6. Characterization of cytochrome c free radical reactions with peptides by mass spectrometry
7. Site-specific spin trapping of tyrosine radicals in the oxidation of metmyoglobin by hydrogen peroxide
8. Detection of myoglobin-derived radicals on reaction of metmyoglobin with hydrogen peroxide and other peroxidic compounds
9. The demonstration of DMPO superoxide adduct upon reperfusion using a low non-toxic concentration
10. Overexpression of human copper, zinc-superoxide dismutase (SOD1) prevents postischemic injury
11. Deleterious effects of oxygen radicals in ischemia/reperfusion: Resolved and unresolved issues
12. Free radicals and their involvement during long-term myocardial ischemia and reperfusion
13. Chronic exposure of neonatal cardiac myocytes to hydrogen peroxide enhances the expression of catalase
14. High sensitivity collisionally-activated decomposition tandem mass spectrometry on a novel quadrupole/orthogonal-acceleration time-of-flight mass spectrometer
15. Spin trapping in electrochemistry. 2. Aqueous and nonaqueous electrochemical characterizations of spin traps
16. Inhibition of radical adduct reduction and reoxidation of the corresponding hydroxylamines in in vivo spin trapping of carbon tetrachloride-derived radicals
17. Mechanism-based chemopreventive strategies against etoposide-induced acute myeloid leukemia: Free radical/antioxidant approach
18. The effects of myoglobin and apomyoglobin on the formation and stability of the hydroxyl radical adduct of 5,5′-dimethyl-1-pyrroline-N-oxide
19. Decay studies of DMPO spin adducts of free radicals produced by reactions of metmyoglobin and methemoglobin with hydrogen peroxide
20. Fenton's reagent revisited