In vitro proliferation and differentiation of human mesenchymal stem cells on hydroxyapatite versus human demineralised bone matrix with and without osteogenic protein-1

Expert Opinion on Biological Therapy

Volumn 9, Issue 1, 2009, Pages 9-19

  Tsiridis, Eleftherios ^a,b,c   Ali, Zubier ^b,c   Bhalla, Amit ^b,c   Gamie, Zakareya ^a   Heliotis, Manolis ^c,d   Gurav, Neelam ^b,c   Deb, Sanjukta ^c   DiSilvio, Lucy ^b,c  

^a LEEDS GENERAL INFIRMARY   (United Kingdom)

^b ROYAL FREE AND UNIVERSITY COLLEGE MEDICAL SCHOOL   (United Kingdom)

^c UNIVERSITY OF LONDON   (United Kingdom)

^d ST MARK S HOSPITAL   (United Kingdom)

Author keywords

BMP 7; Bone substitutes; DBM; Hydroxyapatite; In vitro; OP 1; Osteogenesis; Revision arthroplasty

Indexed keywords

ALKALINE PHOSPHATASE; DNA; HYDROXYAPATITE; OSTEOGENIC PROTEIN 1;

ADULT; ALLOGRAFT; ARTICLE; BONE MATRIX; CASE REPORT; CELL DIFFERENTIATION; CELL PROLIFERATION; HUMAN; IN VITRO STUDY; INTERMETHOD COMPARISON; MALE; MESENCHYMAL STEM CELL; SCANNING ELECTRON MICROSCOPY; CELL CULTURE; COMPARATIVE STUDY; CYTOLOGY; HISTOLOGY; METABOLISM;

ADULT; ALKALINE PHOSPHATASE; BONE DEMINERALIZATION TECHNIQUE; BONE MATRIX; BONE MORPHOGENETIC PROTEIN 7; CELL DIFFERENTIATION; CELL PROLIFERATION; CELLS, CULTURED; DURAPATITE; HUMANS; MALE; MESENCHYMAL STEM CELLS; MICROSCOPY, ELECTRON, SCANNING;

EID: 58149387259     PISSN: 14712598     EISSN: None     Source Type: Journal    
DOI: 10.1517/14712590802622473     Document Type: Article

References (44)

1. Gie GA, Linder L, Ling RS, et al. Impacted cancellous allografts and cement for revision total hip arthroplasty. J Bone Joint Surg Br 1993 ; 75 : 14-21 (Pubitemid 23052407)
2. Slooff TJ, Buma P, Schreurs BW, et al. Acetabular and femoral reconstruction with impacted graft and cement. Clin Orthop Relat Res 1996 ; 108-115 (Pubitemid 26071756)
3. Oakes DA, Cabanela ME. Impaction bone grafting for revision hip arthroplasty: biology and clinical applications. J Am Acad Orthop Surg 2006 ; 14 : 620-628 (Pubitemid 44916626)
4. VandeVord PJ, Nasser S, Wooley PH. Immunological responses to bone soluble proteins in recipients of bone allografts. J Orthop Res 2005 ; 23 : 1059-1064 (Pubitemid 41245429)
5. Zou S, Dodd RY, Stramer SL, Strong DM. Probability of viremia with HBV, HCV, HIV, and HTLV among tissue donors in the United States. N Engl J Med 2004 ; 351 : 751-759 (Pubitemid 39095310)
6. Ahlmann E, Patzakis M, Roidis N, et al. Comparison of anterior and posterior iliac crest bone grafts in terms of harvest-site morbidity and functional outcomes. J Bone Joint Surg Am 2002 ; 84-A : 716-720 (Pubitemid 34925028)
7. Arts JJ, Gardeniers JW, Welten ML, et al. No negative effects of bone impaction grafting with bone and ceramic mixtures. Clin Orthop Relat Res 2005 ; 438 : 239-247 (Pubitemid 41254343)
8. Arts JJ, Walschot LH, Verdonschot N, et al. Biological activity of tri-calciumphosphate/ hydroxyl-apatite granules mixed with impacted morsellized bone graft. A study in rabbits. J Biomed Mater (Pubitemid 46685117)
9. Res B Appl Biomater 2007 ; 81 : 476-485
10. Fujishiro T, Bauer TW, Kobayashi N, et al. Histological evaluation of an impacted bone graft substitute composed of a combination of mineralized and demineralized allograft in a sheep vertebral bone defect. J Biomed Mater Res A 2007 ; 82 : 538-544 (Pubitemid 47258568)
11. Grimm B, Miles AW, Turner IG. Optimizing a hydroxyapatite/tricalcium- phosphate ceramic as a bone graft extender for impaction grafting. J Mater Sci Mater Med 2001 ; 12 : 929-934 (Pubitemid 34062113)
12. van Haaren EH, Smit TH, Phipps K, et al. Tricalcium-phosphate and hydroxyapatite bone-graft extender for use in impaction grafting revision surgery. An in vitro study on human femora. J Bone Joint Surg Br 2005 ; 87 : 267-271 (Pubitemid 40278031)
13. Jensen TB, Overgaard S, Lind M, et al. Osteogenic protein-1 increases the fixation of implants grafted with morcellised bone allograft and ProOsteon bone substitute: an experimental study in dogs. J Bone Joint Surg Br 2007 ; 89 : 121-6 • Hydroxyapatite (ProOsteon) granules used in a canine model with OP-1 with results similar to allograft.
14. McGee MA, Findlay DM, Howie DW, et al. The use of OP-1 in femoral impaction grafting in a sheep model. J Orthop Res 2004 ; 22 : 1008-1015 (Pubitemid 39149165)
15. Soballe K, Jensen TB, Mouzin O, et al. Differential effect of a bone morphogenetic protein-7 (OP-1) on primary and revision loaded, stable implants with allograft. J Biomed Mater Res A 2004 ; 71 : 569-76 •• A combination of allograft with OP-1 in a canine model improved implant fixation.
16. Buma P, Arts JJ, Gardeniers JW, et al. No effect of bone morphogenetic protein-7 (OP-1) on the incorporation of impacted bone grafts in a realistic acetabular model. J Biomed Mater Res B Appl Biomater 2008 ; 84 : 231-239
17. Hannink G, Aspenberg P, Schreurs BW, Buma P. High doses of OP-1 inhibit fibrous tissue ingrowth in impaction grafting. Clin Orthop Relat Res 2006 ; 452 : 250-259 (Pubitemid 44690677)
18. Hannink G, Schreurs BW, Buma P. No positive effects of OP-1 device on the incorporation of impacted graft materials after 8 weeks: a bone chamber study in goats. Acta Orthop 2007 ; 78 : 551-558 (Pubitemid 350032292)
19. Tagil M, Jeppsson C, Wang JS, Aspenberg P. No augmentation of morselized and impacted bone graft by OP-1 in a weight-bearing model. Acta Orthop Scand 2003 ; 74 : 742-8 •• A rabbit knee prosthesis model with impaction grafting and OP-1. No improvements in bone mineral density were found around the implant.
20. Karrholm J, Hourigan P, Timperley J, Razaznejad R. Mixing bone graft with OP-1 does not improve cup or stem fixation in revision surgery of the hip: 5-year follow-up of 10 acetabular and 11 femoral study cases and 40 control cases. Acta Orthop 2006 ; 77 : 39-48 •• A clinical study using impaction allografting and OP-1. Absence of improved clinical outcome prompted discontinuation of the study. (Pubitemid 43879153)
21. Tsiridis E, Ali Z, Bhalla A, et al. In vitro and in vivo optimization of impaction allografting by demineralization and addition of rh-OP-1. J Orthop Res 2007 ; 25 : 1425-1437
22. Tsiridis E, Bhalla A, Ali Z, et al. Enhancing the osteoinductive properties of hydroxyapatite by the addition of human mesenchymal stem cells, and recombinant human osteogenic protein-1 (BMP-7) in vitro. Injury 2006 ; 37 (Suppl 3): S25-32 (Pubitemid 46127412)
23. Jaiswal N, Haynesworth SE, Caplan AI, Bruder SP. Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J Cell Biochem 1997 ; 64 : 295-312 (Pubitemid 27065443)
24. Urist MR. Bone: formation by autoinduction. Science 1965 ; 150 : 893-899
25. Ripamonti U. Bone induction in nonhuman primates. An experimental study on the baboon. Clin Orthop Relat Res 1991 ; 284-294
26. Rago R, Mitchen J, Wilding G. DNA fluorometric assay in 96-well tissue culture plates using Hoechst 33258 after cell lysis by freezing in distilled water. Anal Biochem 1990 ; 191 : 31-34
27. Ahmed SA, Gogal RM Jr, Walsh JE. A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H]thymidine incorporation assay. J Immunol Methods 1994 ; 170 : 211-224 (Pubitemid 24122510)
28. Sabokbar A, Millett PJ, Myer B, Rushton N. A rapid, quantitative assay for measuring alkaline phosphatase activity in osteoblastic cells in vitro. Bone Miner 1994 ; 27 : 57-67 (Pubitemid 24319734)
29. Bae HW, Zhao L, Kanim LE, et al. Intervariability and intravariability of bone morphogenetic proteins in commercially available demineralized bone matrix products. Spine 2006 ; 31 : 1299-306; discussion 307-308 (Pubitemid 43833072)
30. Lee YP, Jo M, Luna M, et al. The efficacy of different commercially available demineralized bone matrix substances in an athymic rat model. J Spinal Disord Tech 2005 ; 18 : 439-444 (Pubitemid 44378137)
31. Bostrom MP, Yang X, Kennan M, et al. An unexpected outcome during testing of commercially available demineralized bone graft materials: how safe are the nonallograft components? Spine 2001 ; 26 : 1425-1428
32. Cullinane DM, Lietman SA, Inoue N, et al. The effect of recombinant human osteogenic protein-1 (bone morphogenetic protein-7) impregnation on allografts in a canine intercalary bone defect. J Orthop Res 2002 ; 20 : 1240-1245 (Pubitemid 35363981)
33. Jensen TB, Overgaard S, Lind M, et al. Osteogenic protein 1 device increases bone formation and bone graft resorption around cementless implants. Acta Orthop Scand 2002 ; 73 : 31-39 (Pubitemid 34218819)
34. Lian JB, Stein GS. Concepts of osteoblast growth and differentiation: basis for modulation of bone cell development and tissue formation. Crit Rev Oral Biol Med 1992 ; 3 : 269-305
35. Poynton AR, Lane JM. Safety profile for the clinical use of bone morphogenetic proteins in the spine. Spine 2002 ; 27 : S40-8 (Pubitemid 34912618)
36. Han B, Tang B, Nimni ME. Quantitative and sensitive in vitro assay for osteoinductive activity of demineralized bone matrix. J Orthop Res 2003 ; 21 : 648-654 (Pubitemid 36763488)
37. Marks T, Wingerter S, Franklin L, et al. Histological and radiographic comparison of allograft substitutes using a continuous delivery model in segmental defects. Biomed Sci Instrum 2007 ; 43 : 194-199 (Pubitemid 46668207)
38. Coathup M, Smith N, Kingsley C, et al. Impaction grafting with a bone-graft substitute in a sheep model of revision hip replacement. J Bone Joint Surg Br 2008 ; 90 : 246-253 (Pubitemid 351303358)
39. Munro NA, Downing MR, Meakin JR, et al. A hydroxyapatite graft substitute reduces subsidence in a femoral impaction grafting model. Clin Orthop Relat Res 2007 ; 455 : 246-52 • Evidence was obtained that hydroxyapatite is mechanically competent to replace allograft.
40. Takigami H, Kumagai K, Latson L, et al. Bone formation following OP-1 implantation is improved by addition of autogenous bone marrow cells in a canine femur defect model. J Orthop Res 2007 ; 25 : 1333-1342 (Pubitemid 47523363)
41. Santoni BG, Pluhar GE, Motta T, Wheeler DL. Hollow calcium phosphate microcarriers for bone regeneration: in vitro osteoproduction and ex vivo mechanical assessment. Biomed Mater Eng 2007 ; 17 : 277-289 (Pubitemid 47357152)
42. Korda M, Blunn G, Goodship A, Hua J. Use of mesenchymal stem cells to enhance bone formation around revision hip replacements. J Orthop Res 2008 ; 26 : 880-885
43. Mushipe MT, Chen X, Jennings D, Li G. Cells seeded on MBG scaffold survive impaction grafting technique: Potential application of cell-seeded biomaterials for revision arthroplasty. J Orthop Res 2006 ; 24 : 501-507 (Pubitemid 43773012)
44. Korda M, Blunn G, Phipps K, et al. Can mesenchymal stem cells survive under normal impaction force in revision total hip replacements? Tissue Eng 2006 ; 12 : 625-633