Ex vivo imaging of motor axon dynamics in murine triangularis sterni explants

Nature Protocols

Volumn 3, Issue 10, 2008, Pages 1645-1653

  Kerschensteiner, Martin ^a   Reuter, Miriam S ^b   Lichtman, Jeff W ^c   Misgeld, Thomas ^b  

^a UNIVERSITY OF MUNICH   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^c HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL; ARTICLE; CYTOLOGY; IMMUNOHISTOCHEMISTRY; INNERVATION; METHODOLOGY; MOTONEURON; MOUSE; NERVE FIBER; PHYSIOLOGY; SKELETAL MUSCLE; TISSUE CULTURE TECHNIQUE; TRANSGENIC MOUSE;

ANIMALS; AXONS; IMMUNOHISTOCHEMISTRY; MICE; MICE, TRANSGENIC; MOTOR NEURONS; MUSCLE, SKELETAL; TISSUE CULTURE TECHNIQUES;

EID: 58149337412     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2008.160     Document Type: Article

References (27)

1. McArdle, J.J.: Advantages of the triangularis sterni muscle of the mouse for investigations of synaptic phenomena. J. Neurosci. Methods 4,109-115 (1981).
2. Brigant, J.L. and Mallart, A.: Presynaptic currents in mouse motor endings. J. Physiol. 333, 619-636 (1982).
3. Angaut-Petit, D. and Mallart, A.: Electrical activity of mouse motor endings during muscle reinervation. Neurosci. 16, 1047-1056 (1985).
4. Angaut-Petit, D.: Electrophysiological and morphological studies ofa motor nerve in 'motorendplate disease' of the mouse. Proc. Soc. Lond. B Biol. Sci. 215, 117-125 (1982).
5. Bournaud, R. and Mallart, A.: Potassium channel blockers and impulse propagation in murine motorendplate disease. Muscle Nerve 10, 1-5 (1987).
6. Bishop, D.L., Misgeld, T., Walsh, M.K., Gan, W.B. and Lichtman, J.W.: Axon branch removal at developing synapses by axosome shedding. Neuron 44, 651-661 (2004).
7. Misgeld, T., Kerschensteiner, M., Bareyre, F.M., Burgess, R.W. and Lichtman, J.W.: Imaging axonal transport of mitochondria in vivo. Nat. Methods 4, 559-561 (2007).
8. Duron, B., Jung-Caillol, M.C. and Marlot, D.: Myelinated nerve fiber supply and muscle spindles in the respiratory muscles of cat: quantitative study. Anat Embryol. (Berl) 152, 171-192 (1978).
9. Keller-Peck, C.R.: Asynchronous synapse elimination in neonatal motor units: studies using GFP transgenic mice. Neuron 31, 381-394 (2001).
10. Livet, J.: Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature 450, 56-62 (2007).
11. Parson, S.H.: Axotomy-dependent and -independent synapse elimination in organ cultures of Wld(s) mutant mouse skeletal muscle. J. Neurosci. Res. 76, 64-75 (2004).
12. Walsh, M.K. and Lichtman, J.W.: Imaging the development of the neuromuscular junction. In Imaging in Neuroscience and Development (eds. Yuste, R. and Konnerth, A.) 215-223 (2005).
13. Walsh, M.K. and Lichtman, J.W.: In vivo time-lapse imaging of synaptic takeover associated with naturally occurring synapse elimination. Neuron 37, 67-73 (2003).
14. Schaefer, A.M., Sanes, J.R. and Lichtman, J.W.: A compensatory subpopulation of motor neurons inamouse model ofamyotrophic lateral sclerosis. J. Comp. Neurol. 490, 209-219 (2005).
15. Sanes, J.R. and Lichtman, J.W.: Development of the vertebrate neuromuscular junction. Annu. Rev. Neurosci. 22, 389-442 (1999).
16. Lichtman, J.W. and Sanes, J.R.: Watching the neuromuscularjunction. J. Neurocytol. 32, 767-775 (2003).
17. Lichtman, J.W. and Colman, H.: Synapse elimination and indelible memory. Neuron 25, 269-278 (2000).
18. Feng, G.: Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28, 41-51 (2000).
19. De Paola, V., Arber, S. and Caroni, P.: AMPA receptors regulate dynamic equilibrium of presynaptic terminals in mature hippocampal networks. Nat. Neurosci. 6, 491-500 (2003).
20. Misgeld, T. and Kerschensteiner, M.: In vivo imaging ofthe diseased nervous system. Nat. Rev. Neurosci. 7, 449-463 (2006).
21. Zuo, Y.: Fluorescent proteins expressed in mouse transgenic lines mark subsets of glia, neurons, macrophages, and dendritic cells for vital examination. J. Neurosci. 24, 10999-11009 (2004).
22. Noguchi, S., Wakabayashi-Takai, E., Sasaoka, T. and Ozawa, E.: Analysis ofthe spatial, temporal and tissue-specific transcription of gamma-sarcoglycan gene using a transgenic mouse. FEBS Lett. 495, 77-81 (2001).
23. Jung, S.: Analysis of fractalkine receptor CX(3)CR1 function by targeted deletion and green fluorescent protein reporter gene insertion. Mol. Cell Biol. 20, 4106-4114 (2000).
24. Marques, M.J., Conchello, J.A. and Lichtman, J.W.: From plaque to pretzel: fold formation and acetylcholine receptor loss at the developing neuromuscular junction. J. Neurosci. 20, 3663-3675 (2000).
25. Parson, S.H., Mackintosh, C.L. and Ribchester, R.R.: Elimination of motor nerve terminals in neonatal mice expressing a gene for slow Wallerian degeneration (C57Bl/Wlds). Eur. J. Neurosci. 9, 1586-1592 (1997).
26. Wyatt, R.M. and Balice-Gordon, R.J.: Heterogeneity in synaptic vesicle release at neuromuscular synapses of mice expressing synaptop Hluorin. J. Neurosci. 28, 325-335 (2008).
27. Lichtman, J.W., Magrassi, L. and Purves, D.: Visualization ofneuromuscularjunctions over periods of several months in living mice. J. Neurosci. 7, 1215-1222 (1987).