Silencing of ZnT1 reduces Zn2+ efflux in cultured cortical neurons

Neuroscience Letters

Volumn 450, Issue 2, 2009, Pages 206-210

  Qin, Yan ^a   Thomas, Dustin ^a   Fontaine, Charles P ^a   Colvin, Robert A ^a  

^a OHIO UNIVERSITY   (United States)

Author keywords

shRNA interference; Zinc homeostasis; Zinc transport

Indexed keywords

ANTIBODY; EDETIC ACID; SHORT HAIRPIN RNA; ZINC ION;

ANIMAL CELL; ARTICLE; CELL LEVEL; CONCENTRATION (PARAMETERS); GENE FUNCTION; GENE SILENCING; GENETIC TRANSFECTION; IMMUNOFLUORESCENCE; KNOCKOUT GENE; MICROFLUOROMETRY; NERVE CELL CULTURE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; ZNT1 GENE;

ANIMALS; CELLS, CULTURED; CEREBRAL CORTEX; EMBRYO, MAMMALIAN; INDOLES; LINEAR MODELS; MEMBRANE PROTEINS; NEURONS; RATS; RNA, SMALL INTERFERING; ZINC;

EID: 58149328388     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.neulet.2008.11.069     Document Type: Article

References (27)

1. G.K. Andrews, H. Wang, S.K. Dey, R.D. Palmiter, Mouse zinc transporter 1 gene provides an essential function during early embryonic development, Genesis (New York, N.Y.: 2000) 40(2) (2004) 74-81.
2. Bender F.L.P., Fischer M., Funk N., Orel N., Rethwilm A., and Sendtner M. High-efficiency gene transfer into cultured embryonic motoneurons using recombinant lentiviruses. Histochem. Cell Biol. 127 4 (2007) 439-448
3. Chao Y., and Fu D. Kinetic study of the antiport mechanism of an Escherichia coli zinc transporter, ZitB. J. Biol. Chem. 279 13 (2004) 12043-12050
4. Chi Z., Wang X., Wang Z., Gao H., Dahlstrom A., and Huang L. Zinc transporter 7 is located in the cis-Golgi apparatus of mouse choroid epithelial cells. Neuroreport 17 17 (2006) 1807-1811
5. 2+ homeostasis in neurons from experimental and modeling studies. Am. J. Cell Physiol. 294 3 (2008) C726-C742
6. Colvin R.A., Laskowski M., and Fontaine C.P. Zinquin identifies subcellular compartmentalization of zinc in cortical neurons. Relation to the trafficking of zinc and the mitochondrial compartment. Brain Res. 1085 1 (2006) 1-10
7. Cousins R.J., and McMahon R.J. Integrative aspects of zinc transporters. J. Nutr. 130 5 (2000) 1384S-S1387
8. Cragg R.A., Christie G.R., Phillips S.R., Russi R.M., Kury S., Mathers J.C., Taylor P.M., and Ford D. A novel zinc-regulated human zinc transporter, hZTL1, is localized to the enterocyte apical membrane. J. Biol. Chem. 277 25 (2002) 22789-22797
9. Huang L., Kirschke C.P., and Gitschier J. Functional characterization of a novel mammalian zinc transporter, ZnT6. J. Biol. Chem. 277 29 (2002) 26389-26395
10. Junghans D., Chauvet S., Buhler E., Dudley K., Sykes T., and Henderson C.E. The CES-2-related transcription factor E4BP4 is an intrinsic regulator of motoneuron growth and survival. Development 131 18 (2004) 4425-4434
11. Kim A.H., Sheline C.T., Tian M., Higashi T., McMahon R.J., Cousins R.J., and Choi D.W. L-type Ca(2+) channel-mediated Zn(2+) toxicity and modulation by ZnT-1 in PC12 cells. Brain Res. 886 1/2 (2000) 99-107
12. Langmade S.J., Ravindra R., Daniels P.J., and Andrews G.K. The transcription factor MTF-1 mediates metal regulation of the mouse ZnT1 gene. J. Biol. Chem. 275 44 (2000) 34803-34809
13. Lin W., Mohandas B., Fontaine C.P., and Colvin R.A. Release of intracellular Zn(2+) in cultured neurons after brief exposure to low concentrations of exogenous nitric oxide. Biometals 20 6 (2007) 891-901
14. Liuzzi J.P., Blanchard R.K., and Cousins R.J. Differential regulation of zinc transporter 1,2 and 4 mRNA expression by dietary zinc in rats. J. Nutr. 131 1 (2001) 46-52
15. M. Lu, D. Fu, Structure of the zinc transporter YiiP, Science (New York, N.Y.) 317 (5845) (2007) 1746-1748.
16. Manev H., Kharlamov E., Uz T., Mason R.P., and Cagnoli C.M. Characterization of zinc-induced neuronal death in primary cultures of rat cerebellar granule cells. Exp. Neurol. 146 1 (1997) 171-178
17. Martin J.L., Stork C.J., and Li Y.V. Determining zinc with commonly used calcium and zinc fluorescent indicators, a question on calcium signals. Cell Calcium 40 4 (2006) 393-402
18. Ohana E., Sekler I., Kaisman T., Kahn N., Cove J., Silverman W.F., Amsterdam A., and Hershfinkel M. Silencing of ZnT-1 expression enhances heavy metal influx and toxicity. J. Mol. Med. 84 9 (2006) 753-763
19. Palmiter R.D., and Findley S.D. Cloning and functional characterization of a mammalian zinc transporter that confers resistance to zinc. EMBO J. 14 4 (1995) 639-649
20. Palmiter R.D., and Huang L. Efflux and compartmentalization of zinc by members of the SLC30 family of solute carriers. Pflugers Archiv. Eur. J. Physiol. 447 5 (2004) 744-751
21. 2+ efflux in cultured cortical neurons. J. Neurochem. 208 (2008) 1304-1313
22. Segal D., Ohana E., Besser L., Hershfinkel M., Moran A., and Sekler I. A role for ZnT-1 in regulating cellular cation influx. Biochem. Biophys. Res. Commun. 323 4 (2004) 1145-1150
23. Sekler I., Moran A., Hershfinkel M., Dori A., Margulis A., Birenzweig N., Nitzan Y., and Silverman W.F. Distribution of the zinc transporter ZnT-1 in comparison with chelatable zinc in the mouse brain. J. Comp. Neurol. 447 3 (2002) 201-209
24. Sensi S.L., and Jeng J. Rethinking the excitotoxic ionic milieu: the emerging role of Zn(2+) in ischemic neuronal injury. Curr. Mol. Med. 4 2 (2004) 87-111
25. Tsuda M., Imaizumi K., Katayama T., Kitagawa K., Wanaka A., Tohyama M., and Takagi T. Expression of zinc transporter gene, ZnT-1, is induced after transient forebrain ischemia in the gerbil. J. Neurosci. 17 17 (1997) 6678-6684
26. Washbourne P., and McAllister A.K. Techniques for gene transfer into neurons. Curr. Opin. Neurobiol. 12 5 (2002) 566-573
27. Wiesenhofer B., and Humpel C. Lipid-mediated gene transfer into primary neurons using FuGene: comparison to C6 glioma cells and primary glia. Exp. Neurol. 164 1 (2000) 38-44