Mutagenesis of longer inserts by the ligation of two PCR fragments amplified with a mutation primer

Journal of Bioscience and Bioengineering

Volumn 107, Issue 1, 2009, Pages 95-97

  Kato, Yu ^a   Arakawa, Noriaki ^a   Masuishi, Yusuke ^a   Kawasaki, Hiroshi ^a   Hirano, Hisashi ^a  

^a YOKOHAMA CITY UNIVERSITY   (Japan)

Author keywords

Deletion; Expression vector; Insertion; Mutagenesis; Polymerase chain reaction (PCR); Substitution; Yeast

Indexed keywords

DELETION; EXPRESSION VECTOR; INSERTION; POLYMERASE CHAIN REACTION (PCR); SUBSTITUTION;

COSMIC RAYS; DNA; GENES; MUTAGENESIS; NUCLEIC ACIDS; ORGANIC ACIDS; POLYMERIZATION; PUMPS; YEAST;

VECTORS;

CLONING, MOLECULAR; DNA; DNA MUTATIONAL ANALYSIS; DNA PRIMERS; GENE DELETION; GENETIC TECHNIQUES; GENETIC VECTORS; HUMANS; MODELS, GENETIC; MUTAGENESIS; MUTAGENESIS, SITE-DIRECTED; MUTATION; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE;

EID: 58149291456     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2008.09.003     Document Type: Article

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