Site-directed mutagenesis study of the antibody 2D7 which catalyzes a reaction for insertion of Cu2+ into mesoporphyrin

Journal of Bioscience and Bioengineering

Volumn 99, Issue 3, 2005, Pages 222-229

  Hosomi, Naoki ^a   Kawamura Konishi, Yasuko ^a   Kawano, Ryota ^a   Fujii, Ikuo ^b   Suzuki, Haruo ^a  

^a KITASATO UNIVERSITY   (Japan)

^b Osaka Prefecture University   (Japan)

Author keywords

Catalytic antibody; Fluorescence quenching; Metalation; Porphyrin; Site directed mutagenesis; Transition state stabilization

Indexed keywords

AMINO ACIDS; ANTIBODIES; CATALYSIS; CATALYST ACTIVITY; COPPER; IONS; REACTION KINETICS;

CATALYTIC ACTIVITY; MUTANTS; N-METHYL MESOPORPHYRIN; SITE-DIRECTED MUTAGENESIS;

MUTAGENESIS;

CATALYTIC ANTIBODY; COPPER ION; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN HEAVY CHAIN; MESOPORPHYRIN; PAPAIN;

ARTICLE; CATALYSIS; CIRCULAR DICHROISM; COMPLEMENTARITY DETERMINING REGION; DISSOCIATION CONSTANT; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE; IMMUNOBLOTTING; MOLECULAR MODEL; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; SITE DIRECTED MUTAGENESIS;

EID: 17644412825     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.99.222     Document Type: Article

References (18)

1. Tramontano, A., Janda, K. D., and Lerner, R. A.: Catalytic antibodies. Science, 234, 1566-1570 (1986).
2. Pollack, S. J., Jacobs, J. W., and Schultz, P. G.: Selective chemical catalysis by an antibody. Science, 234, 1570-1573 (1986).
3. Suzuki, H.: Recent advances in abzyme studies. J. Biochem., 115, 623-628 (1994).
4. Lerner, R. A., Benkovic, S. J., and Schultz, P. G.: At the crossroads of chemistry and immunology: catalytic antibodies. Science, 252, 659-667 (1991).
5. Kawamura-Konishi, Y., Hosomi, N., Neya, S., Sugano, S., Funasaki, N., and Suzuki, H.: Kinetic characterization of antibody-catalyzed insertion of a metal ion into porphyrin. J. Biochem., 119, 857-862 (1996).
6. Kawamura-Konishi, Y., Sasaki, R., Sugiyama, M., Hashimoto, H., Kamo, T., Hosomi, N., Yamazaki, M., Tashiro, H., and Suzuki, H.: Key residue responsible for catalytic activities in the antibodies elicited against N-methyl mesoporphyrin. J. Mol. Catal. B. Enzym., 24-25, 99-109 (2003).
7. Shirai, H., Kidera, A., and Nakamura, H.: Structural classification of CDR-H3 in antibodies. FEBS Lett., 399, 1-8 (1996).
8. Furukawa, K., Shirai, H., Azuma, T., and Nakamura, H.: A role of the third complementarity-determining region in the affinity maturation of an antibody. J. Biol. Chem., 276, 27622-27628 (2001).
9. Miyashita, H., Hara, T., Tanimura, R., Fukuyama, S., Cagnon, C., Kohara, A., and Fujii, I.: Site-directed mutagenesis of active site contact residues in a hydrolytic abzyme: evidence for an essential histidine involved in transition state stabilization. J. Mol. Biol., 267, 1247-1257 (1997).
10. Cagnon, C., Valverde, V., and Masson, J. M.: A new family of sugar-inducible expression vectors for Escherichia coli. Protein Eng., 4, 843-847 (1991).
11. Campbell, D. A., Gong, B., Kochersperger, L. M., Yonkovich, S., Gallop, M. A., and Schultz, P. G.: Antibody-catalyzed prodrug activation. J. Am. Chem. Soc., 116, 2165-2166 (1994).
12. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
13. Kawamura-Konishi, Y., Aoki, T., Satoh, N., Katagiri, M., and Suzuki, H.: Chemical modification of a catalytic antibody that accelerates insertion of a metal ion into porphyrin: essential amino acid residues for the catalytic activity. J. Mol. Catal. B. Enzym., 31, 9-17 (2004).
14. Yin, J., Andryski, S. E., Beuscher, A. E., IV, Stevens, R. C., and Schultz, P. G.: Structural evidence for substrate strain in antibody catalysis. Proc. Natl. Acad. Sci. USA, 100, 856-861 (2003).
15. Urbaneja, M. A. and Chapman, D.: The binding of phosphorylcholine- carrying antigens to the anti-phosphorylcholine monoclonal antibody TEPC 15. A fluorescence spectroscopic study. Biochim. Biophys. Acta, 998, 85-90 (1989).
16. Komissarov, A. A., Calcutt, M. J., Marchbank, M. T., Peletskaya, E. N., and Deutscher, S. L.: Equilibrium binding studies of recombinant anti-single-stranded DNA Fab. J. Biol. Chem., 271, 12241-12246 (1996).
17. Stewart, J. D. and Benkovic, S. J.: Transition-state stabilization as a measure of the efficiency of antibody catalysis. Nature, 375, 388-391 (1995).
18. Angeles, T. S., Smith, R. G., Darsley, M. J., Sugasawara, R., Sanchez, R. I., Kenten, J., Schultz, P. G., and Martin, M. T.: Isoabzymes: structurally and mechanistically similar catalytic antibodies from the same immunization. Biochemistry, 32, 12128-12135 (1993).