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Volumn 322, Issue 5908, 2008, Pages 1705-1710

Regulation of dendritic cell migration by CD74, the MHC class II-associated invariant chain

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; CD74 ANTIGEN; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2; MYOSIN II; UNCLASSIFIED DRUG;

EID: 58149247727     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1159894     Document Type: Article
Times cited : (237)

References (23)
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    • Microchannels enable cell confinement during motion and therefore mimic the microenvironment encountered by DCs in the constrained interstitial spaces of peripheral tissues and lymphoid organs. They impose a directional migration to cells, which facilitates the extraction of measurable parameters. DCs entered and moved spontaneously and bidirectionally along microchannels, with their cell body being constrained during motion (fig. S4, Time-lapse movies obtained with WT, Ii, and CatS, DCs were analyzed by drawing kymographs and extracting cell positioning and instantaneous velocity data for individual migrating cells with a specialized computer program figs. S4 and S5
    • -/- DCs were analyzed by drawing kymographs and extracting cell positioning and instantaneous velocity data for individual migrating cells with a specialized computer program (figs. S4 and S5).
  • 23
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    • The authors thank T. Makushok for setting up the cell migration microchannel system; A. Azicune for cell micropatterning techniques; W. Faigle and D. Lowe for proteomics analysis; C. Recchi for help with gelatin-FITC degradation experiments; H. Overkleeft for synthesizing the LHVS; J. Roger for providing magnetic nanoparticles; M. Leberre, D. Baigl, and Y. Chen for help with microfabrication; and S. Amigorena, M. Sixt, P. Benaroch, V. Soumelis, C. Hivroz, and I. Fernandez for advice on the manuscript. A.-M.L.-D. thanks Y. Bellaïche for help with manuscript redaction. G.F.-A. was supported by a fellowship from the Ministère Français de la Recherche and, together with M.H, from the Association pour la Recherche contre le Cancer (ARC, P.V. and M.-I.Y. benefited from ECOS-Comisíon Nacional de Investigacíon Científica y Technológica, Gobierno de Chile fellowship CO3S01, Institut Curie and Inserm fellowships, respectively. V.S. was part of t
    • The authors thank T. Makushok for setting up the cell migration microchannel system; A. Azicune for cell micropatterning techniques; W. Faigle and D. Lowe for proteomics analysis; C. Recchi for help with gelatin-FITC degradation experiments; H. Overkleeft for synthesizing the LHVS; J. Roger for providing magnetic nanoparticles; M. Leberre, D. Baigl, and Y. Chen for help with microfabrication; and S. Amigorena, M. Sixt, P. Benaroch, V. Soumelis, C. Hivroz, and I. Fernandez for advice on the manuscript. A.-M.L.-D. thanks Y. Bellaïche for help with manuscript redaction. G.F.-A. was supported by a fellowship from the Ministère Français de la Recherche and, together with M.H., from the Association pour la Recherche contre le Cancer (ARC). P.V. and M.-I.Y. benefited from ECOS-Comisíon Nacional de Investigacíon Científica y Technológica, Gobierno de Chile fellowship (CO3S01), Institut Curie and Inserm fellowships, respectively. V.S. was part of the Leonardo Da Vinci project (Unipharma-Graduates, Sapienza University of Rome). This work was funded in part by grants from the ARC, Fondecyt (Chile) to M.-R.B. (1060834) and M.R. (1060253); Agence Nationale pour la Recherche (ANR-06-PCVI-0010) and Human Frontiers Science Program (RGY53/2007) to M.P.


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