Quantification of Fusarium graminearum in harvested grain by real-time polymerase chain reaction to assess efficacies of fungicides on fusarium head blight, deoxynivalenol contamination, and yield of winter wheat

Phytopathology

Volumn 99, Issue 1, 2009, Pages 95-100

  Zhang, Y J ^a   Fan, P S ^a   Zhang, X ^a   Chen, C J ^a   Zhou, M G ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

Author keywords

Mycotoxins

Indexed keywords

FUNGAL DNA; FUNGICIDE; TRICHOTHECENE DERIVATIVE; VOMITOXIN;

ARTICLE; CHEMISTRY; DRUG EFFECT; FOOD CONTAMINATION; FUSARIUM; ISOLATION AND PURIFICATION; MICROBIOLOGY; POLYMERASE CHAIN REACTION; WHEAT;

DNA, FUNGAL; FOOD CONTAMINATION; FUNGICIDES, INDUSTRIAL; FUSARIUM; POLYMERASE CHAIN REACTION; TRICHOTHECENES; TRITICUM;

FUSARIUM; GIBBERELLA ZEAE; TRITICUM AESTIVUM;

EID: 58149239888     PISSN: 0031949X     EISSN: None     Source Type: Journal    
DOI: 10.1094/PHYTO-99-1-0095     Document Type: Article

References (39)

1. Bai, G. H., and Shaner, G. 1996. Variation in Fusarium graminearum and cultivar resistance in wheat scab. Plant Dis. 80:975-979.
2. Bertelsen, J. R., De-Neergaard, E., and Smedegaard-Petersen, V. 2001. Fungicidal effects of azoxystrobin and epoxiconazole on phyllosphere fungi, senescence and yield of winter wheat. Plant Pathol. 50:190-205.
3. Boyacioglu, D., Hettiarachchy, N. S., and Stack, R. W. 1992. Effects of three systemic fungicides on deoxynivalenol (vomitoxin) production by Fusarium graminearum in wheat. Can. J. Plant Sci. 72:93-101.
4. Chen, L. F., Bai, G. H., and Desjardins, A. E. 2000. Recent advances in wheat head scab research in China. Published online by the National Agricultural Library.
5. Chen, Y., Chen, C. J., Wang, J. X., Jin, L. H., and Zhou, M. G. 2007. Induction and characterization of nitrate nonutilizing mutants of Fusarium graminearum resistant to fungicide Js399-19. Sci. Agric. Sin. 40:735-740.
6. Covarelli, L., Turner, A. S., and Nicholson, P. 2004. Repression of deoxynivalenol accumulation and expression of Tri genes in Fusarium culmorum by fungicides in vitro. Plant Pathol. 53:22-28.
7. D'Mello, J. P. F., Macdonald, A. M. C., Postel, D., Dijksma, W. T. P., Dujardin, A., and Placinta, C. M. 1998. Pesticide use and mycotoxin production in Fusarium and Aspergillus phytopathogens. Eur. J. Plant Pathol. 104:741-751.
8. Doohan, F. M., Parry, D. W., Jenkinson, P., and Nicholson, P. 1998. The use of species-specific PCR-based assays to analyse Fusarium ear blight of wheat. Plant Pathol. 47:197-205.
9. Doohan, F. M., Parry, D. W., and Nicholson, P. 1999. Fusarium ear blight of wheat: The use of quantitative PCR and visual disease assessment in studies of disease control. Plant Pathol. 48:209-217.
10. Doohan, F. M., Weston, G., Rezanoor, H. N., Parry, D. W., and Nicholson, P. 1999. Development and use of a reverse transcription PCR assay to study expression of Tri5 by Fusarium species in vitro and in planta. Appl. Environ. Microbiol. 65:3850-3854.
11. Dyer, R. B., Kendra, D. F., and Brown, D. W. 2007. Real-time PCR assay to quantify Fusarium graminearum wild-type and recombination mutant DNA in plant material. J. Microbiol. Methods 67:534-542.
12. Edwards, S. G., Pirgozliev, S. R., Hare, M. C., and Jenkinson, P. 2001. Quantification of trichothecene-producing Fusarium species in harvested grain by competitive PCR to determine efficacies of fungicides against Fusarium head blight of winter wheat. Appl. Environ. Microbiol. 67:1575-1580.
13. Gilbert, J., Abramson, D., McCallum, B., and Clear, R. 2001. Comparison of Canadian Fusarium graminearum isolates for aggressiveness, vegetative compatibility, and production of ergosterol and mycotoxins. Mycopathologia 153:209-215.
14. Gilbert, J., and Tekauz, A. 2000. Recent developments in research in Fusarium head blight of wheat in Canada. Can. J. Plant Pathol. 22:1-8.
15. Goswami, R. S., and Kistler, H. C. 2005. Pathogenicity and in planta mycotoxin accumulation among members of the Fusarium graminearum species complex on wheat and rice. Phytopathology 95:1397-1404.
16. Haidukowski, M., Pascale, M., Perrone, G., Pancaldi, D., Campagna, C., and Visconti, A. 2004. Effect of fungicides on the development of Fusarium head blight, yield and deoxynivalenol accumulation in wheat inoculated under field conditions with Fusarium graminearum and Fusarium culmorum. J. Sci. Food Agric. 82:191-198.
17. Han, Q. M., Kang, Z. S., and Duan, S. K. 2003. The control effects of two fungicides, tebuconazole and metconazole to Fusarium head blight of wheat. Acta Phytophylacica Sin. 30:339-440.
18. Hilton, A. J., Jenkinson, P., Hollins, T. W., and Parry, D. W. 1999. Relationship between cultivar height and severity of Fusarium ear blight in wheat. Plant Pathol. 48:202-208.
19. Hormdock, S., Fehrmann, H., and Beck, R. 2000. Effects of field application of tebuconazole on yield, yield components and the mycotoxin content of Fusarium infected wheat grain. J. Phytopathol. 148:1-6.
20. Ioos, R., Belhadj, A., Menez, M., and Faure, A. 2005. The effects of fungicide on Fusarium spp. and Microdochium nivale and their associated trichothecene mycotoxins in French naturally-infected cereal grains. Crop Prot. 24:894-902.
21. Ji, L., Cao, K., Hu, T., and Wang, S. 2007. Determination of deoxynivalenol and nivalenol chemotypes of Fusarium graminearum isolates from China by PCR assay. J. Phytopathol. 155:505-512.
22. Kunz, S., DeIISng, H., and Mendgen, K. 1997. Acquisition of resistance to sterol demethylation inhibitors by populations of Venturia inaequalis. Phytopathology 87:1272-1278.
23. Li, H. K., Diao, Y. M., Wang, J. X., Chen, C. J., Ni, J. P., and Zhou, M. G. 2008. Js399-19, a new fungicide against wheat scab. Crop Prot. 27:273-278.
24. Liu, W. Z., Langseth, W., Skinnes, H., Elen, O. N., and Sundheim, L. 1997. Comparison of visual head blight ratings, seed infection levels, and deoxynivalenol production for assessment of resistance in cereals inoculated with Fusarium culmorum. Eur. J. Plant Pathol. 10:589-595.
25. Matthies, A., and Buchenauer, H. 2000. Effect of tebuconazole (Folicur) and prochloraz (Sportac) treatments on Fusarium head scab development, yield and deoxynivalenol (DON) content in grains of wheat following artificial inoculation with Fusarium culmorum. J. Plant Dis. Prot. 107:33-52.
26. McMullen, M., Jones, R., and Gallenberg, D. 1997. Scab of wheat and barley: a re-emerging disease of devastating impact. Plant Dis. 81:1340-1348.
27. Mesterhazy, A., Bartok, T., and Lamper, C. 2003. Influence of wheat cultivar, species of Fusarium, and isolate aggressiveness on the efficiency of fungicides for control of Fusarium head blight. Plant Dis. 87:1107-1115.
28. Mesterhazy, A., Bartok, T., Mirocha, C. G., and Komoroczy, R. 1999. Nature of wheat resistance to Fusarium head blight and the role of deoxynivalenol for breeding. Plant Breed. 118:97-110.
29. Parry, D. W., Jenkinson, P., and McLeod, L. 1995. Fusarium ear blight (scab) in small grain cereals - A review. Plant Pathol. 44:207-238.
30. Pirgozliev, S. R., Edwards, S. G., Hare, M. C., and Jenkinson, P. 2002. Effect of dose rate of azoxystrobin and metconazole on the development of Fusarium head blight and the accumulation of deoxynivalenol (DON) in wheat grain. Eur. J. Plant Pathol. 108:469-478.
31. Placinta, C. M., D'Mello, J. B. F., and Macdonald, A. M. C. 1999. A review of world contamination of cereal grains and animal feed with Fusarium mycotoxins. Anim. Feed Sci. Technol. 78:21-37.
32. Schnerr, H., Vogel, R. F., and Niessen, L. 2002. Correlation between DNA of trichothecene-producing Fusarium species and deoxynivalenol concentrations in wheat-samples. Lett. Appl. Microbiol. 35:121-125.
33. Simpson, D. R., Weston, G. E., Turner, J. A., Jennings, P., and Nicholson, P. 2001. Differential control of head blight pathogens of wheat by fungicides and consequences for mycotoxin contamination of grain. Eur. J. Plant Pathol. 107:421-431.
34. Siranidou, E., and Buchenauer, H. 2001. Chemical control of Fusarium head blight on wheat. J. Plant Dis. Prot. 108:231-243.
35. Stergiopoulos, I., Van-Nistelroy, J. G. M., Kema, G. H. J., and De-Waard, M. A. 2003. Multiple mechanisms account for variation in base-line sensitivity to azole fungicides in field isolates of Mycosphaerella graminicola. Pest Manage. Sci. 59:1333-1343.
36. Sui, K., Li, J., Wei, F., and Zhao, S. C. 2005. Determination of deoxynivalenol in cereal grains by high performance liquid chromatography and verified by high performance liquid chromatography-mass spectrometry. Chin. J. Anal. Chem. 33:1643-1646.
37. Wang, Y. Z., and Miller, J. D. 1994. Toxin producing potential of Fusarium graminearum from China. Acta Mycol. Sin. 13:229-234.
38. Zadoks, J. C., Chang, T. T., and Konzak, C. F. 1974. A decimal code for the growth stages of cereals. Weed Res. 14:415-421.
39. Zhou, M. G., and Wang, J. X. 2001. Study on sensitivity base-line of F. graminearum to carbendazim biological characters of MBC-resistant strains. Acta Phytopathol. Sin. 31:365-370.