Quantification of Trichothecene-Producing Fusarium Species in Harvested Grain by Competitive PCR to Determine Efficacies of Fungicides against Fusarium Head Blight of Winter Wheat

Applied and Environmental Microbiology

Volumn 67, Issue 4, 2001, Pages 1575-1580

  Edwards, S G ^a   Pirgozliev, S R ^a   Hare, M C ^a   Jenkinson, P ^a  

^a HARPER ADAMS UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIFUNGAL AGENT; FUNGAL DNA; LYASE; TRICHODIENE SYNTHETASE; TRICHOTHECENE DERIVATIVE; VOMITOXIN;

ARTICLE; BIOSYNTHESIS; DRUG EFFECT; ENZYMOLOGY; EVALUATION; FUSARIUM; GENETICS; METABOLISM; METHODOLOGY; MICROBIOLOGY; PLANT DISEASE; POLYMERASE CHAIN REACTION; WHEAT;

ANTIFUNGAL AGENTS; CARBON-CARBON LYASES; DNA, FUNGAL; FUSARIUM; PLANT DISEASES; POLYMERASE CHAIN REACTION; TRICHOTHECENES; TRITICUM;

EID: 0035316253     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.67.4.1575-1580.2001     Document Type: Article

References (48)

1. Abramson, D., R. M. Clear, and D. M. Smith. 1993. Trichothecene production by Fusarium spp. isolated from Manitoba grain. Can. J. Plant Pathol. 15:147-152.
2. Abramson, D., Z. Gan, R. M. Clear, J. Gilbert, and R. R. Marquardt. 1998. Relationships among deoxynivalenol, ergosterol and the Fusarium exoantigens in Canadian hard and soft wheat. Int. J. Food Microbiol. 45:217-224.
3. Boyacioglu, D., N. S. Hettiarachchy, and R. W. Stack. 1992. Effect of three systemic fungicides on deoxynivalenol (vomitoxin) production by Fusarium graminearum in wheat. Can. J. Plant Sci. 72:93-101.
4. Burgess, L. W., T. A. Klein, W. L. Bryden, and N. F. Tobin. 1987. Head blight of wheat caused by Fusarium graminearum Group 1 in NSW in 1983. Aust. J. Plant Pathol. 16:72-78.
5. de Galich, M. T. V. 1996. Fusarium head blight in Argentina, p. 19-28. In H. J. Duhin, L. Gilchrist, J. Reeves, and A. McNab (ed.), Fusarium head scab: global status and future prospects. International Maize and Wheat Improvement Center, Mexico City, Mexico.
6. Desjardins, A. E., T. M. Hohn, and S. McCormick. 1992. Effect of gene disruption of trichodiene synthase on the virulence of Gibberella pulicaris. Mol. Plant-Microbe Interact. 5:214-222.
7. D'Mello, J. P. F., A. M. C. Macdonald, D. Postel, W. T. P. Dijksma, A. Dujardin, and C. M. Placinta. 1998. Pesticide use and mycotoxin production in Fusarium and Aspergillus phytopathogens. Eur. J. Plant Pathol. 104:741-751.
8. D'Mello, J. P. F., C. M. Placinta, and A. M. C. Macdonald. 1999. Fusarium mycotoxins: a review of global implications for animal health, welfare and productivity. Anim. Feed Sci. Tech. 80:183-205.
9. Doohan, F. M., D. W. Parry, P. Jenkinson, and P. Nicholson. 1998. The use of species-specific PCR-based assays to analyse Fusarium ear blight of wheat. Plant Pathol. 47:197-205.
10. Doohan, F. M., D. W. Parry, and P. Nicholson. 1999. Fusarium ear blight of wheat: the use of quantitative PCR and visual disease assessment in studies of disease control. Plant Pathol. 48:209-217.
11. Doohan, F. M., G. Weston, H. N. Rezanoor, D. W. Parry, and P. Nicholson. 1999. Development and use of a reverse transcription-PCR assay to study expression of Tri5 by Fusarium species in vitro and in planta. Appl. Environ. Microbiol. 65:3850-3854.
12. Fekete, C., A. Logrieco, G. Giczey, and L. Hornok. 1997. Screening of fungi for the presence of trichodiene synthase encoding sequence by hybridization to the Tri5 gene cloned from Fusarium poae. Mycopathologia 138:91-97.
13. Förster, E. 1994. An improved general method to generate internal standards for competitive PCR. BioTethniques 16:18-20.
14. Gareis, M., and J. Ceynowa. 1994. Influence of the fungicide Matador (tebuconazole and triadimenol) on mycotoxin production by Fusarium culmorum. Z. Lebensm. Unters. Forsch. 198:244-248.
15. Gilbert, J., and A. Tekauz. 2000. Recent developments in research in fusarium head blight of wheat in Canada. Can. J. Plant Pathol. 22:1-8.
16. Hasan, H. A. H. 1993. Fungicide inhibition of aflatoxins, diacetoxyscirpenol and zearalenone production. Folia Microbiol. 38:295-298.
17. Hilton, A. J., P. Jenkinson, T. W. Hollins, and D. W. Parry. 1999. Relationship between cultivar height and severity of Fusarium ear blight in wheat. Plant Pathol. 48:202-208.
18. Hohn, T. M., and P. D. Beremand. 1989. Isolation and nucleotide sequence of sesquiterpene cyclase gene from the trichothecene-producing fungus Fusarium sporotrichioides. Gene 79:131-138.
19. Hohn, T. M., A. E. Desjardins, and S. P. McCormick. 1993. Analysis of Tox5 gene expression in Gibberella pulicaris strains from different trichothecene production phenotypes. Appl. Environ. Microbiol. 59:2359-2363.
20. Hormdock, S., H. Fehrmann, and R. Beck. 2000. Effects of field application of tebuconazole on yield, yield components and the mycotoxin content of Fusarium-infected wheat grain. J. Phytopathol. 148:1-6.
21. Hussein, H. M., M. Baxter, I. G. Andrew, and R. A. Franich. 1991. Mycotoxin production by Fusarium species isolated from New Zealand maize fields. Mycopathologia 113:506-511.
22. Jones, R. K., and C. J. Mirocha. 1999. Quality parameters in small grains from Minnesota affected by fusarium head blight. Plant Dis. 83:506-511.
23. Lacy, J., G. L. Bateman, and C. J. Mirocha. 1999. Effects of infection time and moisture on development of ear blight and deoxynivalenol production by Fusarium spp. in wheat. Ann. Appl. Biol. 134:277-283.
24. Lemmens, M., R. Josephs, R. Schumacher, H. Grausgruber, H. Buerstmayr, P. Ruckenbauer, G. Neuhold, M. Fidesser, and R. Krska. 1997. Head blight (Fusarium spp.) on wheat: investigations on the relationship between disease symptoms and mycotoxin content. Cereal Res. Commun. 25:459-465.
25. Liu, W. Z., W. Langseth, H. Skinnes, O. N. Elen, and L. Sundheim. 1997. Comparison of visual head blight ratings, seed infection levels, and deoxynivalenol production for assessment of resistance in cereals inoculated with Fusarium culmorum. Eur. J. Plant Pathol. 103:589-595.
26. Martin, R. A., and H. W. Johnston. 1982. Effects and control of Fusarium diseases of cereal grains in the Atlantic Provinces. Can. J. Plant Pathol. 4:210-216.
27. Matthies, A., and H. Buchenauer. 1996. Investigations on the action of different active ingredients on the biosynthesis of mycotoxins in Fusarium culmorum and Fusarium graminearum. p. 199-204. In H. Lyr, P. E. Russell, and H. D. Sisler (ed.), Modern fungicides and antifungal compounds. Intercept Ltd., Andover, United Kingdom.
28. McMullen, M., R. Jones, and D. Gallenberg. 1997. Scab of wheat and barley: a re-emerging disease of devastating impact. Plant Dis. 81:1340-1348.
29. Mesterhazy, A., T. Bartok, C. G. Mirocha, and R. Komoroczy. 1999. Nature of wheat resistance to Fusarium head blight and the role of deoxynivalenol for breeding. Plant Breeding 118:97-110.
30. Moss, M. O., and J. M. Frank. 1985. Influence of the fungicide tridemorph on T2 toxin production of Fusarium sporotrichioides. Trans. Br. Mycol. Soc. 84:585-590.
31. Niessen, L. M., and R. F. Vogel. 1998. Group specific PCR-detection of potential trichothecene-producing Fusarium-species in pure culture and cereal samples. Syst. Appl. Microbiol. 21:618-631.
32. Parry, D. W., T. R. Pettitt, P. Jenkinson, and A. K. Lees. 1994. The cereal Fusarium complex, p. 301-320. In P. Blakeman and B. Williamson (ed.), Ecology of plant pathogens. CAB International, Wallingford, United Kingdom.
33. Parry, D. W., P. Jenkinson, and L. McLeod. 1995. Fusarium ear blight (scab) in small grain cereals - a review. Plant Pathol. 44:207-238.
34. Placinta, C. M., A. M. C. Macdonald, J. B. F. D'Mello, and R. Harling. 1996. The influence of carbendazim on mycotoxin production in Fusarium sporotrichioides, p. 415-416. In Proceedings of the Brighton Crop Protection Conference 1996: Pests and Diseases. British Crop Protection Council, Farnham, United Kingdom.
35. Placinta, C. M., J. B. F. D'Mello, and A. M. C. Macdonald. 1999. A review of world contamination of cereal grains and animal feed with Fusarium mycotoxins. Anim. Feed Sci. Technol. 78:21-37.
36. Proctor, R. H., T. M. Hohn, and S. P. McCormick. 1995. Reduced virulence of Gibberella zeae caused by disruption of a trichothecene toxin biosynthetic gene. Mol. Plant-Microbe Interact. 8:593-601.
37. Saur, L. 1991. Sources of resistance to headblight caused by Fusarium culmorum in bread wheat and related species. Agronomie 11:535-541.
38. Smith, P. 1997. Ph.D. thesis. Trichodiene synthase and the role of trichothecenes in Fusarium spp. University of East Anglia, Norwich, United Kingdom.
39. Snijders, C. H. A. 1990. Fusarium head blight and mycotoxin contamination of wheat, a review. Neth. J. Plant Pathol. 96:187-198.
40. Snijders, C. H. A., and J. Perkowski. 1990. Effects of headblight caused by Fusarium culmorum on toxin content and weight of wheat kernels. Phytopathology 80:566-570.
41. Suty, A., A. Mauler-Machnik, and R. Courbon. 1996. New findings on the epidemiology of Fusarium ear blight on wheat and its control with tebuconazole, p. 511-516. In Proceedings of the Brighton Crop Protection Conference 1996: Pests and Diseases. British Crop Protection Council, Farnham, United Kingdom.
42. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
43. Ueda, S., and T. Yoshizawa. 1988. Effect of fungicides on abakabi-byo and trichothecene production in wheat and barley. Proc. Jpn. Assoc. Mycotoxicol. 28(Suppl. 1):234-235.
44. Walsh, P. S., D. A. Metzger, and R. Higuchi. 1991. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 10:506-513.
45. Wang, Y. Z. 1996. Epidemiology and management of wheat scab in China, p. 97-105. In H. J. Dubin, L. Gilchrist, J. Reeves, and A. McNab (ed.), Fusarium head scab: global status and future prospects. International Maize and Wheat Improvement Center, Mexico City, Mexico.
46. Whitehead, R. 1999. The United Kingdom pesticide guide 1999. CAB International, Wallingford, United Kingdom.
47. Wong, L. S. L., A. Tekauz, D. Leisle, D. Abramson, and R. I. H. McKenzie. 1992. Prevalence, distribution and importance of Fusarium headblight in wheat in Manitoba. Can. J. Plant Pathol. 14:233-238.
48. Zadoks, J. C., T. T. Chang, and C. F. Konzak. 1974. A decimal code for the growth stages of cereals. Weed Res. 14:415-421.