In vitro differentiation of human calvarial suture derived cells with and without dexamethasone does not induce in vivo-like expression

Journal of Cellular Physiology

Volumn 218, Issue 1, 2009, Pages 183-191

  Coussens, Anna K ^a,b   Hughes, Ian P ^a   Morris, C Phillip ^a   Powell, Barry C ^b,c,d   Anderson, Peter J ^b,c,d  

^a QUEENSLAND UNIVERSITY OF TECHNOLOGY   (Australia)

^b CHILD HEALTH RESEARCH INSTITUTE   (Australia)

^c UNIVERSITY OF ADELAIDE   (Australia)

^d UNIVERSITY OF ADELAIDE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; ASCORBIC ACID; BONE SIALOPROTEIN; COLLAGEN TYPE 1; COLLAGEN TYPE 1 ALPHA 1; COMPLEMENT COMPONENT C1Q; DEXAMETHASONE; FIBROBLAST GROWTH FACTOR 2; GLYCEROL 2 PHOSPHATE; GLYPICAN 3; OSTEOCALCIN; RETINOL BINDING PROTEIN 4; TRANSCRIPTION FACTOR RUNX2; TUMOR NECROSIS FACTOR RECEPTOR ASSOCIATED FACTOR 3; UNCLASSIFIED DRUG; WNT1 PROTEIN; BETA-GLYCEROPHOSPHORIC ACID; C1QTNF3 PROTEIN, HUMAN; GLYCEROPHOSPHATE; PRIMER DNA; TUMOR NECROSIS FACTOR;

ARTICLE; CELL DIFFERENTIATION; CELL STIMULATION; CONTROLLED STUDY; CRANIOFACIAL SYNOSTOSIS; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; IN VIVO STUDY; MALE; OSTEOBLAST; PRIORITY JOURNAL; PROTEIN EXPRESSION; BONE DEVELOPMENT; CELL CULTURE; CRANIAL SUTURE; CULTURE MEDIUM; CYTOLOGY; DRUG EFFECT; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENETICS; INFANT; METABOLISM; NUCLEOTIDE SEQUENCE; PATHOLOGY; PHENOTYPE;

ASCORBIC ACID; BASE SEQUENCE; CELL DIFFERENTIATION; CELLS, CULTURED; CRANIAL SUTURES; CRANIOSYNOSTOSES; CULTURE MEDIA; DEXAMETHASONE; DNA PRIMERS; FIBROBLAST GROWTH FACTOR 2; GENE EXPRESSION; GENE EXPRESSION PROFILING; GLYCEROPHOSPHATES; HUMANS; INFANT; MALE; OSTEOBLASTS; OSTEOGENESIS; PHENOTYPE; TUMOR NECROSIS FACTORS;

EID: 58149147047     PISSN: 00219541     EISSN: None     Source Type: Journal    
DOI: 10.1002/jcp.21586     Document Type: Article

References (44)

1. Anderson PJ, Cox TC, Roscioli T, Elakis G,Smithers L, David DJ, Powell BC. 2007. Somatic FGFR and TWIST mutations are not a common cause of isolated non-syndromic single suture craniosynostosis. J Craniofac Surg 18:312-314.
2. Aubin JE, Triffitt J. 2002. Mesenchymal stem cells and the osteoblast lineage. In: Bilezikian JP, Raisz LG, Rodan GA, Principles of bone biology editors. Academic Press. New York: pp 59-81.
3. Bellows CG, Aubin JE, Heersche JN. 1987. Physiological concentrations of glucocorticoids stimulate formation ofbone nodules from isolated ratcalvaria cells in vitro. Endocrinology 121:1985-1992.
4. Bellows CG, Sodek J, Yao KL, Aubin JE. 1986. Phenotypic differences in subclones and long-term cultures of clonally derived rat bone cell lines. J Cell Biochem 31:153-169.
5. Candeliere GA, Liu F, Aubin JE. 2001. Individual osteoblasts in the developing calvaria express different gene repertoires. Bone 28:351-361.
6. Candeliere GA, Rao Y, Floh A, Sandler SD, Aubin JE. 1999. cDNA fingerprinting of osteoprogenitor cells to isolate differentiation stage-specific genes. Nucleic Acids Res 27:1079-1083.
7. Choi KY, Kim HJ, Lee MH, Kwon TG,Nah HD, Furuichi T, Komori T, Nam SH, Kim YJ, Ryoo HM. 2005. Runx2 regulates FGF2-induced Bmp2 expression during cranial bone development. Dev Dyn 233:115-121.
8. Coussens AK,Hughes IP,Wilkinson CR,Morris CP,Anderson PJ,Powell BC,van Daal A. 2008. Identification of genes differentially expressed by prematurely fused human sutures using a novel in vivo-in vitro approach. Differentiation 76:531-545.
9. Coussens AK,Wilkinson CR,Hughes IP,Morris CP,van Daal A,Anderson PJ, Powell BC. 2007. Unravelling the molecular control of calvarial suture fusion in children with craniosynostosis. BMC Genomics 8:458.
10. de Pollack C, Arnaud E, Renier D, Marie PJ. 1997. Age-related changes in bone formation, osteoblastic cell proliferation, and differentiation during postnatal osteogenesis in human calvaria.J Cell Biochem 64:128-139.
11. de Pollack C, Renier D, Hott M, Marie PJ. 1996. Increased bone formation and osteoblastic cell phenotype in premature cranial suture ossification (craniosynostosis). J Bone Miner Res 11:401-407.
12. Debiais F, Hott M,Graulet AM, Marie PJ. 1998.The effects of fibroblast growth factor-2 on human neonatal calvaria osteoblastic cells are differentiation stage specific. J Bone Miner Res 13:645-654.
13. Ecarot-Charrier B,Glorieux FH,van der Rest M,Pereira G. 1983. Osteoblasts isolated from mouse calvaria initiate matrix mineralization in culture. J Cell Biol 96:639-643.
14. Fakhry A, Ratisoontorn C, Vedhachalam C, Salhab I, Koyama E, Leboy P, Pacifici M, Kirschner RE, Nah HD. 2005. Effects of FGF-2/-9 in calvarial bone cell cultures: Differentiation stage-dependent mitogenic effect, inverse regulation ofBMP-2 and noggin, and enhancement of osteogenic potential. Bone 36:254-266.
15. Franceschi RT. l992. The role ofascorbic acid in mesenchymal differentiation. Nutr Rev 50:65-70.
16. Franceschi RT,Iyer BS.1992.Relationshipbetween collagen synthesisandexpression ofthe osteoblast phenotype in MC3T3-El cells. J Bone Miner Res 7:235-246.
17. Gerber I, Gwynn I. 2001. Influence of cell isolation, cell culture density, and cell nutrition on differentiation ofrat calvarial osteoblast-like cells in vitro. Eur Cell Mater 2:10-20.
18. Guenou H, Kaabeche K, Mee SL, Marie PJ. 2005. A role for fibroblast growth factor receptor-2 in the altered osteoblast phenotype induced by Twist haploinsufficiency in the Saethre-Chotzen syndrome. Hum Mol Genet 14:1429-1439.
19. Hughes FJ, Collyer J, Stanfield M, Goodman SA. 1995. The effects of bone morphogenetic protein-2, -4, and -6 on differentiation ofrat osteoblast cells in vitro. Endocrinology l36:2671-2677.
20. Igarashi M,Kamiya N,Hasegawa M,Kasuya T,Takahashi T,Takagi M. 2004.Inductive effects of dexamethasone on the gene expression of Cbfal, Osterix and bone matrix proteins during differentiation ofcultured primary rat osteoblasts. J Mol Histol 35:3-10.
21. Iseki S, Wilkie AO, Morriss-Kay GM.1999. Fgfrl and Fgfr2have distinctdifferentiation- and proliferation-related roles in the developing mouse skull vault. Development 126:5611-5620.
22. Ishida Y, Heersche JN. 1997. Progesterone stimulates proliferation and differentiation of osteoprogenitor cells in bone cell populations derived from adult female but not from adult male rats. Bone 20:17-25.
23. Kalajzic I, Kalajzic Z, Hurley MM, Lichtler AC, Rowe D. 2003. Stage specific inhibition of osteoblast lineage differentiation by FGF2 and noggin. J Cell Biochem 88:1168-1176.
24. Levenson JM, Sweatt JD.2006. Epigenetic mechanisms:Acommon theme in vertebrate and invertebrate memory formation. Cell Mol Life Sci 63:1009-1016.
25. Liu F, Malaval L, Aubin JE. 1997. The mature osteoblast phenotype is characterized by extensive plasticity. Exp Cell Res 232:97-105.
26. Liu F, Malaval L, Aubin JE. 2003. Global amplification polymerase chain reaction reveals novel transitional stages during osteoprogenitor differentiation. J Cell Sci 116:1787-1796.
27. Lomri A,Lemonnier J,Hott M,deParseval N,Lajeunie E, Munnich A, Renier D,Marie PJ. 1998. Increased calvaria cell differentiation and bone matrix formation induced by fibroblast growth factor receptor 2 mutations in Apert syndrome. J Clin Invest 101:1310-1317.
28. Madras N, Gibbs AL, Zhou Y, Zandstra PW, Aubin JE. 2002. Modeling stem cell development by retrospective analysis of gene expression profiles in single progenitor-derived colonies. Stem Cells 20:230-240.
29. Malaval L, Liu F, Roche P, Aubin JE. 1999. Kinetics of osteoprogenitor proliferation and osteoblast differentiation in vitro. J Cell Biochem 74:6l6-627.
30. Meisinger C,Zeschnigk C,Grothe C.1996. In vivo and in vitro effect of glucocorticoids on fibroblast growth factor (FGF)-2 and FGF receptor 1 expression. J Biol Chem 271:16520-16525.
31. Morriss-Kay GM, Wilkie AO. 2005. Growth ofthe normal skull vault and its alteration in craniosynostosis: Insights from human genetics and experimental studies. JAnat207:637-653.
32. Naderi A, Ahmed AA, Barbosa-Morais NL, Aparicio S, Brenton JD, Caldas C. 2004. Expression microarray reproducibility is improved by optimising purification steps in RNA amplification and labelling. BMC Genomics 5:9.
33. Nefussi JR, Boy-Lefevre ML, Boulekbache H, Forest N. 1985. Mineralization in vitro of matrix formed by osteoblasts isolated by collagenase digestion. Differentiation 29: 160-168.
34. Ogston N, Harrison AJ, Cheung HF, Ashton BA, Hampson G. 2002. Dexamethasone and retinoic acid differentially regulate growth and differentiation in an immortalised human clonal bone marrow stromal cell line with osteoblastic characteristics. Steroids 67:895-906.
35. Opperman LA. 2000. Cranial sutures as intramembranous bone growth sites. Dev Dyn 219:472-485.
36. Owen TA, Aronow M, Shalhoub V, Barone LM, Wilming L, Tassinari MS, Kennedy MB, Pockwinse S, Lian JB, Stein GS. 1990. Progressive development ofthe rat osteoblast phenotype in vitro: Reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol 143:420-430. Ratisoontorn C, Seto ML, Broughton KM, Cunningham ML. 2005. In vitro differentiation profile of osteoblasts derived from patients with Saethre-Chotzen syndrome. Bone 36:627-634.
37. Rice DP, Rice R, Thesleff 1. 2003. Molecular mechanisms in calvarial bone and suture development, and their relation to craniosynostosis. Eur J Orthod 25:139-148.
38. Spector JA,Greenwald JA,Warren SM,Bouletreau PJ,Crisera FE,Mehrara BJ,Longaker MT.2002.Co- cultureofosteoblastswithimmatureduralcellscausesanincreasedrateand degree of osteoblast differentiation. Plast Reconstr Surg 109:631-642; discussion 643-644.
39. Turksen K, Aubin JE. 1991. Positive and negative immunoselection for enrichment of two classes of osteoprogenitor cells. J Cell Biol 114:373-384.
40. Vaes B, Dechering K, van Someren E, Hendriks J, van de Ven C, Feijen A, Mummery C, Reinders M, Olijve W, van Zoelen E, Steegenga W. 2005. Microarray analysis reveals expression regulation of Wnt antagonists in differentiating osteoblasts. Bone 36:803-811.
41. Wang D,Christensen K,Chawla K,Xiao G, Krebsbach PH, Franceschi RT. 1999. Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential. J Bone Miner Res 14:893-903.
42. Wu H, Sun YE. 2006. Epigenetic regulation of stem cell differentiation. Pediatr Res 59:21R-25R.
43. Yousfi M, Lasmoles F, El Ghouzzi V, Marie PJ. 2002. Twist haploinsufficiency in Saethre-Chotzen syndrome induces calvarial osteoblast apoptosis due to increased TNFalpha expression and caspase-2 activation. Hum Mol Genet 11:359-369.
44. Zhou YS, Liu YS, Tan JG. 2006. Is 1, 25-dihydroxyvitamin D3 an ideal substitute for dexamethasone for inducing osteogenic differentiation of human adipose tissue-derived stromal cells in vitro? Chin Med J (Engl) 119:1278-1286.