Major Mo(V) EPR signature of rhodobacter sphaeroides periplasmic nitrate reductase arising from a dead-end species that activates upon reduction. Relation to other molybdoenzymes from the DMSO reductase family

Journal of Physical Chemistry B

Volumn 112, Issue 48, 2008, Pages 15478-15486

  Fourmond, Vincent ^a,b   Burlat, Bénédicte ^a,b   Dementin, Sébastien ^a,b   Arnoux, Pascal ^b,c,d   Sabaty, Monique ^b,c,d   Boiry, Séverine ^b,c,d   Guigliarelli, Bruno ^a,b   Bertrand, Patrick ^a,b   Pignol, David ^b,c,d   Léger, Christophe ^a,b  

^a AIX MARSEILLE UNIVERSITÉ   (France)

^b AIX MARSEILLE UNIVERSITY   (France)

^c IRFM   (France)

^d CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSTS; DIMETHYL SULFOXIDE; ELECTRON RESONANCE; ENZYME ACTIVITY; ENZYMES; MOLYBDENUM; NITRATES; PARAMAGNETIC RESONANCE; PROTEINS; QUANTUM THEORY; VOLTAMMETRY;

ACTIVE SITES; ACTIVE SPECIES; CATALYTIC CYCLES; CATALYTIC PROPERTIES; COFACTOR; COMMON PROPERTIES; EPR SIGNALS; EPR SPECTROSCOPIES; FUNCTIONAL INFORMATIONS; MEMBRANE BOUNDS; MOLYBDOENZYMES; MOLYBDOPTERIN; NITRATE REDUCTASES; PERIPLASMIC; PERIPLASMIC NITRATE REDUCTASES; PH INDEPENDENTS; PROTEIN FILMS; REDOX CYCLING; REDUCTIVE ACTIVATION PROCESSES; REDUCTIVE ACTIVATIONS; REDUCTIVE PROCESSES; RHODOBACTER SPHAEROIDES; SPECTROSCOPIC SIGNATURES; SPHAEROIDES; TRANSFER REACTIONS;

ELECTRON SPIN RESONANCE SPECTROSCOPY;

EID: 57949107626     PISSN: 15206106     EISSN: None     Source Type: Journal    
DOI: 10.1021/jp807092y     Document Type: Article

References (45)

1. Hille, R. Trends Biochem. Sci. 2002, 27, 360-367.
2. Hille, R. Chem. Rev. 1996, 96, 2757-2816.
3. George, G.; Hilton, J.; Temple, C.; Prince, R.; Rajagopalan, K. J. Am. Chem. Soc. 1999, 121, 1256-1266.
4. Bray, R.; Adams, B.; Smith, A.; Bennett, B.; Bailey, S. Biochemistry 2000, 39, 11258-11269.
5. George, G.; Hilton, J.; Rajagopalan, K. J. Am. Chem. Soc. 1996, 118, 1113-1117.
6. Bell, A. F.; He, X.; Ridge, J. P.; Hanson, G. R.; McEwan, A. G.; Tonge, P. J. Biochemistry 2001, 40, 440-448.
7. Li, H.-K.; Temple, C.; Rajagopalan, K.; Schindelin, H. J. Am. Chem. Soc 2000, 122, 7673-7680.
8. Zhang, L.; Nelson, K.; Rajagopalan, K.; George, G. Inorg. Chem. 2008, 47, 1074-1078.
9. Tavares, P.; Pereira, A.; Moura, J.; Moura, I. J. Inorg. Biochem. 2006, 100, 2087-2100.
10. Arnoux, P.; Sabaty, M.; Alric, J.; Frangioni, B.; Guigliarelli, B.; Adriano, J.-M.; Pignol, D. Nat. Struct. Mol. Biol. 2003, 10, 928-934.
11. Butler, C. S.; Ferguson, S. J.; Berks, B. C.; Thomson, A. J.; Cheesman, M. R.; Richardson, D. J. FEBS Lett. 2001, 500, 71-74.
12. Dias, J. M.; Than, M. E.; Humm, A.; Huber, R.; Bourenkov, G. P.; Bartunik, H. D.; Bursakov, S.; Calvete, J.; Caldeira, J.; Carneiro, C.; Moura, J. J.; Moura, I.; Romao, M. J. Structure 1999, 7, 65-79.
13. Najmudin, S.; Gonzalez, P.; Trincao, J.; Coelho, C.; Mukhopadhyay, A.; Cerqueira, N.; Romao, C.; Moura, I.; Moura, J.; Brondino, C. Rom. J. Biol. Inorg. Chem. 2008, 13, 1432-1327.
14. Butler, C.; Charnock, J.; Bennett, B.; Sears, H.; Reilly, A.; Ferguson, S.; Garner, C.; Lowe, D.; Thomson, A.; Berks, B.; Richardson, D. Biochemistry 1999, 38, 9000-9012.
15. Bennett, B.; Benson, N.; McEwan, A. G.; Bray, R. C Eur. J. Biochem. 1994, 225, 321-331.
16. Gangeswaran, R.; Lowe, D. J.; Eady, R. R. Biochem. J. 1993, 289 (Pt 2), 335-342.
17. Dementin, S.; Arnoux, P.; Frangioni, B.; Grosse, S. L; Burlat, B.; Guigliarelli, B.; Sabaty, M.; Pignol, D. Biochemistry 2007,46, 9713-9721.
18. Jepson, B. J. N.; Mohan, S.; Clarke, T. A.; Gates, A. J.; Cole, J. A.; Butler, C. S.; Butt, J. N.; Hemmings, A. M.; Richardson, D. J. J. Biol. Chem. 2007, 282, 6425-6437.
19. Bennett, B.; Berks, B. C.; Ferguson, S. J.; Thomson, A. J.; Richardson, D. J. Eur. J. Biochem. 1994, 226, 789-798.
20. Butler, C. S.; Charnock, J. M.; Garner, C. D.; Thomson, A. J.; Ferguson, S. J.; Berks, B. C.; Richardson, D. J. Biochem. J. 2000, 352, 859-864.
21. Jepson, B. J. N.; Anderson, L. J.; Rubio, L. M.; Taylor, C. J.; Butler, C. S.; Flores, E.; Herrero, A.; Butt, J. N.; Richardson, D. J. J. Biol. Chem. 2004, 279, 32212-32218.
22. Gonzalez, P.; Rivas, M.; Brondino, C.; Bursakov, S.; Moura, I.; Moura, J. J. Biol. Inorg. Chem. 2006, 11, 609-616.
23. Sears, H. J.; Bennett, B.; Spiro, S.; Thomson, A. J.; Richardson, D. J. Biochem. J. 1995, 310, 311-314.
24. Butler, C. S.; Fairhurst, S. A.; Ferguson, S. J.; Thomson, A. J.; Berks, B. C.; Richardson, D. J.; Lowe, D. J. Biochem. J. 2002, 363, 817-823.
25. Bursakov, S.; Liu, M.-Y.; Payne, W. J.; LeGall, J.; Moura, I.; Moura, J. J. Anaerobe 1995, 1, 55-60.
26. Frangioni, B.; Arnoux, P.; Sabaty, M.; Pignol, D.; Bertrand, P.; Guigliarelli, B.; Léger, C. J. Am. Chem. Soc 2004, 126, 1328-9.
27. Field, S. J.; Thornton, N. P.; Anderson, L. J.; Gates, A. J.; Reilly, A.; Jepson, B. J. N.; Richardson, D. J.; George, S. J.; Cheesman, M. R.; Butt, J. N. Dalton Trans. 2005, 3580-3586.
28. Elliott, S. J.; Léger, C.; Pershad, H. R.; Hirst, J.; Heffron, K.; Ginet, N.; Blasco, F.; Rothery, R. A.; Weiner, J. H.; Armstrong, F. A. Biochim. Biophys. Acta 2002, 1555, 54-9.
29. Léger, C.; Elliott, S. J.; Hoke, K. R.; Jeuken, L. J. C.; Jones, A. K.; Armstrong, F. A. Biochemistry 2003, 42, 8653-62.
30. Léger, C.; Bertrand, P. Chem. Rev. 2008, 108, 2379-2438.
31. Bertrand, P.; Frangioni, B.; Dementin, S.; Sabaty, M.; Arnoux, P.; Guigliarelli, B.; Pignol, D.; Léger, C. J. Phys. Chem. B 2007, 111, 10300-10311.
32. Hoke, K.; Cobb, N.; Armstrong, F.; Hille, R. Biochemistry 2004, 43, 1667-1674.
33. An activation faster than about Is would be masked by the charging current, whereas very slow activation could be hidden by the decrease in current resulting from film desorption, which occurs under these conditions with first-order kinetics and a characteristic time of about 3000 s.
34. -1.
35. Frangioni, B. Etude fonctionnelle de la nitrate reductase périplasmique de Rhodobacter sphaeroides par spectroscopic RPE et électrochimie directe. Thesis, Université Aix-Marseille I, 2006.
36. As opposed to electrochemically poised using protein film voltammetry.
37. 38
38. Guigliarelli, B.; Bertrand, P.; More, C.; Papavassiliou, P.; Hatchikian, E. C.; Gayda, J. P. Biochim. Biophys. Acta (BBA) - Bioenergetics 1985, 810, 319-324.
39. Bastian, N. R.; Kay, C. J.; Barber, M. J.; Rajagopalan, K. V. J. Biol. Chem. 1991, 266, 45-51.
40. Hagedoorn, P. L.; Hagen, W. R.; Stewart, L. J.; Docrat, A.; Bailey, S.; CD, C. D. G. FEBS Lett. 2003, 555, 606-610.
41. Gates, A. J.; Richardson, D. J.; Butt, J. N. Biochem. J. 2008, 409, 159-168.
42. Cobb, N.; Conrads, T.; Hille, R. J. Biol. Chem. 2005, 280, 11007-11017.
43. Adams, B.; Smith, A.; Bailey, S.; McEwan, A.; Bray, R. Biochemistry 1999, 38, 8501-8511.
44. Léger, C.; Dementin, S.; Bertrand, P.; Rousset, M.; Guigliarelli, B. J. Am. Chem. Soc. 2004, 126, 12162-12172.
45. Léger, C.; Lederer, F.; Guigliarelli, B.; Bertrand, P. J. Am. Chem. Soc. 2006, 128, 180-187.