Detection of antibodies against SARS-coronavirus using recombinant truncated nucleocapsid proteins by ELISA

Journal of Microbiology and Biotechnology

Volumn 18, Issue 10, 2008, Pages 1717-1721

  Lee, Hyun Kyoung ^a   Lee, Byoung Hee ^b   Dutta, Noton Kumar ^a   Seok, Seung Hyeok ^a   Baek, Min Won ^a   Lee, Hui Young ^a   Kim, Dong Jae ^a   Na, Yi Rang ^a   Noh, Kyoung Jin ^a   Park, Sung Hoon ^a   Kariwa, Hiroaki ^c   Nakauchi, Mina ^c   Mai, Le Quynh ^d   Heo, Suk Jin ^e   Park, Jae Hak ^a  

^a Seoul National University   (South Korea)

^b National Institute of Biological Resources   (South Korea)

^c HOKKAIDO UNIVERSITY   (Japan)

^d NATIONAL INSTITUTE OF HYGIENE AND EPIDEMIOLOGY   (Viet Nam)

^e Korea Food and Drug Administration   (South Korea)

Author keywords

ELISA; SARS; Truncated recombinant nucleocapsid proteins

Indexed keywords

NUCLEOCAPSID PROTEIN; NUCLEOCAPSID PROTEIN 1; NUCLEOCAPSID PROTEIN 2; NUCLEOCAPSID PROTEIN 3; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ANTIBODY DETECTION; ANTIGENICITY; ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; IMMUNE RESPONSE; NONHUMAN; NUCLEOTIDE SEQUENCE; SARS CORONAVIRUS; SENSITIVITY AND SPECIFICITY; WESTERN BLOTTING;

ANTIBODIES, VIRAL; ANTIGENS, VIRAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; HUMANS; NUCLEOCAPSID; RECOMBINANT PROTEINS; SARS VIRUS; SEVERE ACUTE RESPIRATORY SYNDROME;

CORONAVIRUS; SARS CORONAVIRUS;

EID: 57849165832     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Che, X. Y., L. W. Qiu, Z. Y. Liao, Y. D. Wang, K. Wen, Y. X. Pan, et al. 2005. Antigenic cross-reactivity between severe acute respiratory syndrome-associated coronavirus and human coronaviruses 229E and OC43. J. Infect. Dis. 191: 2033-2037.
2. Dutta, N. K., K. Mazumdar, B. H. Lee, M. W. Baek, D. J. Kim, Y. R. Na, et al. 2008. Search for potential target site of nucleocapsid gene for the design of an epitope-based SARS DNA vaccine. Immunol. Lettl. 118: 65-71.
3. Huang, L. R., C. M. Chiu, S. H. Yeh, W. H. Huang, P. R. Hsueh, W. Z. Yang, et al. 2004. Evaluation of antibody responses against SARS coronaviral nucleocapsid or spike proteins by immunoblotting or ELISA. J. Med. Virol. 73: 338-346.
4. Marra, M. A., S. J. M. Jones, C. R. Astell, R. A. Holt, A. Brooks-Wilson, Y. S. N. Butterfield, et al. 2003. The genome sequence of the SARS-associated coronavirus. Science 300: 1399-1404.
5. Reithinger, R., R. J. Quinnell, B. Alexander, and C. R. Davies. 2002. Rapid detection of Leishmania infantum infection in dogs: Comparative study using an immunochromatographic dipstick test, enzyme-linked immunosorbent assay, and PCR. J. Clin. Virol. 40: 2352-2356.
6. Rota, P. A., M. S. Oberste, S. S. Monroe, W. A. Nix, R. Campagnoli, J. P. Icenogle, et al. 2003. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science 300: 1394-1399.
7. Saijio, M., M. Nikura, S. Morikawa, T. G. Ksiazek, R. F. Meyer, C. J. Peters, and I. Kurane. 2001. Enzyme-linked immunosorbent assay for detection of antibodies to Ebola and Marburg viruses using recombinant nucleoproteins. J. Clin. Virol. 39: 1-7.
8. Saijo, M., T. Ogino, F. Taguchi, S. Fukushi, T. Mizutani, T. Notomi, et al. 2005. Recombinant nucleocapsid protein-based IgG enzyme-linked immunosorbent assay for the serological diagnosis of SARS. J. Virol. Methods 125: 181-186.
9. Shang, B., X. Y. Wang, J. W. Yuan, A. Vabret, X. D. Wu, R. F. Yang, et al. 2005. Characterization and application of monoclonal antibodies against N protein of SARS-coronavirus. Biochem. Biophys. Res. Commun. 336: 110-117.
10. Shim, Y. Y., W. S. Shin, G. S. Moon, and K. H. Kim. 2007. Quantitative analysis of phosphinothricin-N-acetyltransferase in genetically modified herbicide tolerant pepper by an enzyme-linked immunosorbent assay. J. Microbiol. Biotechnol. 17: 681-684.
11. Shin, G. C., Y. S. Chung, I. S. Kim, H. W. Cho, and C. Kang. 2006. Preparation and characterization of a novel monoclonal antibody specific to severe acute respiratory syndrome-coronavirus nucleocapsid protein. Virus Res. 122: 109-118.
12. Sizun, J., D. Soupre, M. C. Legrand, J. D. Giroux, S. Rubio, J. M. Cauvin, C. Chastel, D. Alix, and L. de Parscau. 1995. Neonatal nosocomial respiratory infection with coronavirus: A prospective study in a neonatal intensive care unit. Acta Paediatr. 84: 617-620.
13. Sizun, J., M. W. Yu, and P. J. Talbot. 2000. Survival of human coronaviruses 229E and OC43 in suspension and after drying on surfaces: A possible source of hospital-acquired infections. J. Hosp. Infect. 46: 55-60.
14. Timani, K. A., L. Ye, L. Ye, Y. Zhu, Z. Wu, and Z. Gong. 2004. Cloning, sequencing, expression, and purification of SARS-associated coronavirus nucleocapsid protein for serodiagnosis of SARS. J. Clin. Virol. 30: 309-312.
15. Wang, J., J. Wen, J. Li, J. Yin, Q. Zhu, H. Wang, et al. 2003. Assessment of immunoreactive synthetic peptides from the structural proteins of severe acute respiratory syndrome coronavirus. Clin. Chem. 49: 1989-1996.
16. World Health Organization. 2003. WHO post-outbreak biosafety guidelines for handling of SARS-CoV specimens and cultures. Available at http://www.who.int/csr/sars/biosafety2003_12_18/en/.
17. Woo, P. C., S. K. Lau, B. H. Wong, K. H. Chan, W. T. Hui, G. S. Kwan, J. S. Peiris, R. B. Couch, and K. Y. Yuen. 2004. False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide. J. Clin. Microbiol. 42: 5885-5888.
18. Yu, F., M. Q. Le, S. Inoue, H. T. C. Thai, F. Hasebe, M. del Carmen Parquet, and K. Morita. 2005. Evaluation of inapparent nosocomial severe acute respiratory syndrome coronavirus infection in Vietnam by use of highly specific recombinant truncated nucleocapsid protein-based enzyme-linked immunosorbent assay. Clin. Diagn. Lab. Immunol. 12: 848-854.