Quantitative Analysis of phosphinothricin-N-acetyltransferase in genetically modified herbicide tolerant pepper by an Enzyme-linked immunosorbent assay

Journal of Microbiology and Biotechnology

Volumn 17, Issue 4, 2007, Pages 681-684

  Shim, Youn Young ^d   Shin, Weon Sun ^a   Moon, Gi Seong ^b   Kim, Kyung Hwan ^c  

^a Hanyang University   (South Korea)

^b Chungju National University   (South Korea)

^c National Institute of Animal Science   (South Korea)

^d SASKATOON RESEARCH CENTRE   (Canada)

Author keywords

Bar gene; GM pepper; Phosphinothricin N acetyltransferase; Sandwich ELISA

Indexed keywords

ACYLTRANSFERASE; BIALAPHOS; HISTIDINE; PHOSPHINOTHRICIN; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENE OVEREXPRESSION; GENETICALLY MODIFIED CROP; HERBICIDE TOLERANCE; INTERMETHOD COMPARISON; NONHUMAN; PEPPER; PROTEIN PURIFICATION; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY; WESTERN BLOTTING;

ACETYLTRANSFERASES; ANIMALS; CAPSICUM; ENZYME-LINKED IMMUNOSORBENT ASSAY; HERBICIDES; PLANTS, GENETICALLY MODIFIED; RABBITS;

CAPSICUM; ESCHERICHIA COLI;

EID: 34248372107     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Ahmed, F. E. 2002. Detection of genetically modified organisms in foods. Trends Biotechnol. 20: 215-223.
2. James, C. 2004. Preview: Global Status of Commercialized Biotech /GM Crops, ISAAA Briefs, No. 32, ISAAA, New York.
3. Kalogianni, D., P. Zhang, T. Zhang, T. K. Christopoulos, and P. C. Ioannou. 2006. Nanoparticle-based DNA biosensor for visual detection of genetically modified organisms. Biosens. Bioelectron. 21: 1069-1076.
4. Kim, J H., H. S. Song, D. H. Kim, and H. Y. Kim. 2006. Quantification of genetically modified canola GT73 using TaqMan real-time PCR. J. Microbiol. Biotechnol. 16: 1778-1783.
5. Kim, Y. H., S. J. Choi, H. A. Lee, and T. W. Moon. 2006. Quantification of CP4 5-enolpyruvylshikimate-3-phosphate synthase in soybean by two-dimensional gel electrophoresis. J. Microbiol. Biotechnol. 16: 25-31.
6. Kwak, B. Y., S. H. Ko, W. S. Shin, and D. H. Shon. 2003. Analysis of genetically-modified soybean and soybean sprout by Enzyme-Linked Immunosorbent Assay (ELISA). Korean J. Food Sci. Technol. 35: 556-560.
7. Lee, S. H., H. J. Park, S. H. Cho, H. K. Chun, D. H. Kim, T. H. Ruy, and M. C. Cho. 2004. Comparison of major nutrients and mineral contents in genetically modified herbicide-tolerant red pepper and its parental cultivars. Food Sci. Biotechnol. 13: 830-833.
8. Lee, Y. H., S. B. Lee, S. C. Suh, M. O. Byun, and H. I. Kim. 2000. Herbicide resistant cabbage plants by Agrobacterium-mediated transformation. J. Plant Biotechnol. 2: 35-41.
9. Lipp, M., E. Anklam, and J. W. Stave. 2000. Validation of an immunoassay for detection and quantitation of a genetically modified soybean in food and food fractions using reference materials: Interlaboratory study. J. AOAC Int. 83: 919-927.
10. McKenzie M. J., V. Mett, and P. E. Jameson. 2000. Modified ELISA for the detection of neomycin phosphotransferase II in transformed plant species. Plant Cell Rep. 9: 286-289.
11. Rudi, K., I. Rud, and A. Holck. 2003. A novel multiplex quantitative DNA array based PCR (MQDA-PCR) for quantification of transgenic maize in food and feed. Nucleic Acids Res. 31: e62.
12. Shim, Y. Y., D. H. Shon, B. Y. Kwak, J. H. Yu, and K. M. Chee. 2004. Comparison of properties of polyclonal anti-N-acetylchitooligosaccharides and anti-chitooligosaccharides antibodies produced for ELISA. J. Microbiol. Biotechnol. 14: 686-692.
13. Song, H. S., J. W. Kim, D. H. Kim, and H. Y. Kim. 2007. Qualitative and quantitative analysis of genetically modified pepper. J. Microbiol. Biotechnol. (in press)
14. Stave, J. W. 2002. Protein immunoassay methods for detection of biotech crops: Applications, limits, and practical considerations. J. AOAC Int. 85: 780-786.