Identification and characterization of a novel O-superfamily conotoxin from Conus litteratus

Journal of Peptide Science

Volumn 14, Issue 10, 2008, Pages 1077-1083

  Wang, Lei ^a   Pi, Canhui ^a   Liu, Junliang ^a   Chen, Shangwu ^a   Peng, Can ^a   Sun, Dandan ^a   Zhou, Maojun ^a   Xiang, Hui ^a   Ren, Zhenghua ^a   Xu, Anlong ^a  

^a SUN YAT SEN UNIVERSITY   (China)

Author keywords

Conus litteratus; Fusion expression; O superfamily conotoxin; Voltage gated sodium channel; Whole cell patch clamp

Indexed keywords

BLOOD CLOTTING FACTOR 10A; CONOTOXIN; CONOTOXIN 1T6C; CYSTEINE; CYSTEINYLCYSTEINYLCYSTEINYLCYSTEINYLCYSTEINYLCYSTEINE; POLYPEPTIDE; SIGNAL PEPTIDE; SODIUM CHANNEL; THIOREDOXIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DNA LIBRARY; ESCHERICHIA COLI; EXPRESSED SEQUENCE TAG; EXPRESSION VECTOR; GENE EXPRESSION; NERVE CELL; NERVE CELL MEMBRANE STEADY POTENTIAL; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PATCH CLAMP; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PURIFICATION; RAT; SEQUENCE ANALYSIS; SODIUM CURRENT; SPINAL GANGLION; SPRAGUE DAWLEY RAT;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CELLS, CULTURED; CONOTOXINS; CONUS SNAIL; FEMALE; MALE; MOLECULAR SEQUENCE DATA; MULTIGENE FAMILY; NEURONS; RATS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SODIUM CHANNELS;

CONUS LITTERATUS; ESCHERICHIA COLI; RATTUS;

EID: 57849140606     PISSN: 10752617     EISSN: 10991387     Source Type: Journal    
DOI: 10.1002/psc.1044     Document Type: Article

References (25)

1. Olivera BM, Rivier J, Clark C, Ramilo CA, Corpuz GP, Abogadie FC, Mena EE, Woodward SR, Hillyard DR, Cruz LJ. Diversity of Conus neuropeptides. Science 1990; 249: 257-263.
2. Terlau H, Olivera BM. Conus venoms: a rich source of novel ion channel-targeted peptides. Physiol. Rev. 2004; 84(1): 41-68.
3. Olivera BM. E.E. Just Lecture, 1996. Conus venom peptides, receptor and ion channel targets, and drug design: 50 million years of neuropharmacology. Mol. Biol. Cell 1997; 8(11): 2101-2109.
4. Olivera BM, Cruz LJ. Conotoxins, in retrospect. Toxicon 2004; 39(1): 7-14.
5. Luna-Ramírez KS, Aguilar MB, Falcón A, Heimer de la Cotera EP, Olivera BM, Maillo M. An O-conotoxin from the vermivorous Conus spurius active on mice and mollusks. Peptides 2007; 28(1): 24-30.
6. Jiang H, Xu CQ, Wang CZ, Fan CX, Zhao TY, Chen JS, Chi CW. Two novel O-superfamily conotoxins from Conus vexillum. Toxicon 2006; 47(4): 425-436.
7. Daly NL, Ekberg JA, Thomas L, Adams DJ, Lewis RJ, Craik DJ. Structures of muO-conotoxins from Conus marmoreus. Inhibitors of tetrodotoxin (TTX)-sensitive and TTX-resistant sodium channels in mammalian sensory neurons. J. Biol. Chem. 2004; 279(24): 25774-25782.
8. Luo S, Zhangsun D, Feng J, Wu Y, Zhu X, Hu Y. Diversity of the O-superfamily conotoxins from Conus miles. J. Pept. Sci. 2007; 13(1): 44-53.
9. Wermeling DP. Ziconotide, an intrathecally administered N-type calcium channel antagonist for the treatment of chronic pain. Pharmacotherapy 2005; 25: 1084-1094.
10. Pi C, Liu J, Peng C, Liu Y, Jiang X, Zhao Y, Tang S, Wang L, Dong M, Chen S, Xu A. Diversity and evolution of conotoxins based on gene expression profiling of Conus Litteratus. Genomics 2006; 88: 809-819.
11. Pi C, Liu J,Wang L, Jiang X, Liu Y, Peng C, Chen S, Xu A. Soluble expression, purification and functional identification of a disulfide-rich conotoxin derived from Conus litteratus. J. Biotechnol. 2007; 128: 184-193.
12. Wang MJ, Li ZW. Neurokinin B protentiates ATP-activated currents in rat DRG neurons. Brain Res. 2001; 923: 157-162.
13. Nicholson GM, Walsh T. Characterization of the effects of robustoxin, the lethal neurotoxin form the Sydney funnel-web spider Atrax robustus, on sodium channel activation and inactivation. Pflugers Arch. 1998; 436: 117-126.
14. Liu J, Wu Qi, Pi C, Zhao Y, Zhou M, Wang L, Chen S, Xu A. Isolation and characterization of a T-superfamily conotoxin from Conus litteratus with targeting tetrodoxin-sensitive sodium channels. Peptides 2007; 28(12): 2313-2319.
15. Ramilo CA, Zafaralla GC, Nadasdi L, Hammerland LG, Yoshikami D, Gray WR, Kristipati R, Ramachandran J, Miljanich G, Olivera BM. Novel alpha- and omega-conotoxins from Conus striatus venom. Biochemistry 1992; 31(41): 9919-9926.
16. Lewis RJ, Nielsen KJ, Craik DJ, Loughnan ML, Adams DA, Sharpe IA, LuchianT, Adams DJ, Bond T, Thomas L, Jones A, Matheson JL, Drinkwater R, Andrews PR, Alewood PF. Novel omega-conotoxins from Conus catus discriminate among neuronal calcium channel subtypes. J. Biol. Chem. 2000; 275(45): 35335-35344.
17. Adams DJ, Smith AB, Schroeder CI, Yasuda T, Lewis RJ. Omega-conotoxin CVID inhibits a pharmacologically distinct voltage-sensitive calcium channel associated with transmitter release from preganglionic nerve terminals. J. Biol. Chem. 2003; 278(6): 4057-4062.
18. Ekberg J, Craik DJ, Adams DJ. Conotoxin modulation of voltage-gated sodium channels. Int. J. Biochem. Cell Biol. 2007 (in press). DOI: 10.1016/j.biocel.2007.08.017.
19. Buczek O, Bulaj G, Olivera BM. Conotoxins and the posttranslational modification of secreted gene products. Cell Mol. Life Sci. 2005; 62(24): 3067-3079.
20. Cestèle S, Catterall WA. Molecular mechanisms of neurotoxin action on voltage-gated sodium channels. Biochimie 2000; 82(9-10): 883-892.
21. French RJ, Zamponi GW. Voltage-gated sodium and calcium channels in nerve, muscle, and heart. IEEE Trans. Nanobioscience 2005; 4(1): 58-69.
22. Leipold E, Hansel A, Olivera BM, Terlau H, Heinemann SH. Molecular interaction of delta-conotoxins with voltage-gated sodium channels. FEBS Lett. 2005; 579(18): 3881-3884.
23. Bulaj G, Olivera BM. Folding of conotoxins: formation of the native disulfide bridges during chemical synthesis and biosynthesis of conus peptides. Antioxid. Redox Signal. 2008; 10(1): 141-156.
24. Zhan J, Chen X, Wang C, Qiu J, Ma F, Wang K, Zheng S. A fusion protein of conotoxin MVIIA and thioredoxin expressed in Escherichia coli has significant analgesic activity. Biochem. Biophys. Res. Commun. 2003; 311(2): 495-500.
25. Wang X, Xie LP, Li QA, Zhang RQ, Zhou XW, Huang PT. Effect of O- superfamily conotoxin SO3 on synchronized spontaneous calcium spikes in cultured hippocampal networks. Cell Biol. Toxicol. 2008; 24(1): 11-17.