Characterisation of the effects of robustoxin, the lethal neurotoxin from the Sydney funnel-web spider Atrax robustus, on sodium channel activatiom and inactivation

Pflugers Archiv European Journal of Physiology

Volumn 436, Issue 1, 1998, Pages 117-126

  Nicholson, Graham M ^a   Walsh, Robyn ^a   Little, Michelle J ^a   Tyler, Margaret I ^b  

^a UNIVERSITY OF TECHNOLOGY SYDNEY   (Australia)

^b CSIRO   (Australia)

Author keywords

Atracotoxin Ar1; Dorsal root ganglion neurons; Funnel web spider; Robustoxin; Sodium channel; Sodium current; Spider toxin; Whole cell patch clamp

Indexed keywords

NEUROTOXIN; ROBUSTOXIN; SODIUM CHANNEL; SPIDER VENOM; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL MEMBRANE POTENTIAL; CHANNEL GATING; CONTROLLED STUDY; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; RAT; SODIUM CURRENT; SPINAL GANGLION; TOXICITY TESTING;

AMINO ACID SEQUENCE; ANIMALS; DRUG RESISTANCE; ELECTRIC CONDUCTIVITY; GANGLIA, SPINAL; KINETICS; MOLECULAR SEQUENCE DATA; NEUROTOXINS; PATCH-CLAMP TECHNIQUES; RATS; SODIUM CHANNELS; SPIDER VENOMS; TETRODOTOXIN;

EID: 0031978687     PISSN: 00316768     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004240050612     Document Type: Article

References (47)

1. Atchison WD, Luke VS, Narahashi T, Vogel SM (1986) Nerve membrane sodium channels as the target site of brevetoxins at neuromuscular junctions. Br J Pharmacol 89: 731-738
2. Baden DG, Rein KS, Gawley RE, Jeglitsch G, Adams DJ (1994) Is the A-Ring lactone of brevetoxin PbTX-3 required for sodium channel orphan receptor binding and activity. Natural Toxins 2: 212-221
3. Benoit E, Legrand AM, Dubois JM (1986) Effects of ciguatoxin on current and voltage clamped frog myelinated nerve fibre. Toxicon 24: 357-364
4. Beress L, Ritter R, Ravens U (1982) The influence of rate of electrical stimulation on the effects of the Anemonia sulcata toxin ATX II in guinea pig papillary muscle. Eur J Pharmacol 79: 265-272
5. Bezanilla F, Armstrong CM (1977) Inactivation of sodium channels. I. Sodium current experiments. J Gen Physiol 70: 594-566
6. Brown MR, Sheumack DD, Tyler MI, Howden MEH (1988) Amino acid sequence of versutoxin, a lethal neurotoxin from the venom of the funnel-web spider Atrax versutus. Biochem J 250: 401-405
7. Brill DM, Wasserstrom JA (1986) Intracellular Na and the positive inotropic effect of veratridine and cardiac glycoside in sheep Purkinje fibers. Circ Res 58: 109-119
8. Catterall WA (1992) Cellular and molecular biology of voltage-gated sodium channels. Physiol Rev 72: 15-48
9. Fainzilber M, Kofman O, Zlotkin E, Gordon D (1994) A new neurotoxin receptor site on sodium channel is identified by a conotoxin that affects sodium channel inactivation in molluscs, and acts as an antagonist in rat brain. J Biol Chem 269: 2574-2580
10. Fainzilber M, Lodder J, Kits KS, Kofman O, Vinnitsky I, Van Rietschoten J, Zlotkin E, Gordon D (1995) A new conotoxin affecting sodium current inactivation interacts with the δ-conotoxin receptor site. J Biol Chem 270: 1123-1129
11. 3) cells. J Physiol (Lond) 356: 565-585
12. Fletcher JI, Smith R, O'Donoghue S, Nilges M, Connor M, Howden MEH, Christie MJ, King GF (1997) The structure of a novel insecticidal neurotoxin, ω-atracotoxin-HV1, from the venom of an Australian funnel web spider. Nature Struct Biol 4: 559-566
13. Fletcher JI, Chapman BE, Mackay JP, Howden MEH, King GF (1998) The structure of versutoxin (δ-atracotoxin-Hv1): implications for binding of site-toxins to the voltage-gated sodium channel. Structure 5: 1525-1535
14. Gage PW, Spence I (1977) The origin of the muscle fasciculation caused by funnel-web spider venom. Aust J Exp Biol Med Sci 55: 453-461
15. Gonoi T, Hille B (1987) Gating of Na channels: inactivation modifiers discriminate among models. J Gen Physiol 89: 253-274
16. Gray MR (1988) Aspects of the systematics of the Australian tunnel-web spiders (Araneae: Hexathelidae: Atracinae) based upon morphological and electrophoretic data. In: Austin AD, Heather NW (eds) Australian arachnology. The Australian Entomological Society, Brisbane, Australia. 5: 113-125
17. Gusovsky F, Rossignol DP, McNeal ET, Daly JW (1988). Pumiliotoxin B binds to a site on the voltage-dependent sodium channel that is allosterically coupled to other binding sites. Proc Natl Acad Sci USA 85:1272-1276
18. Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch 391: 85-100
19. Hille B (1977) Local anesthetics: hydrophilic and Hydrophobic pathways for drug-receptor reaction. J Gen Physiol 69: 497-515
20. Huang JMC, Wu CH, Baden DG (1984) Depolarizing action of a red-tide dinoflagellate brevetoxin on axonal membranes. J Pharmacol Exp Ther 229: 615-621
21. Huang L-Y M, Yatani A, Brown AM (1987) The properties of batrachotoxin-modified cardiac Na channels, including state-dependent block by tetrodotoxin. J Gen Physiol 90: 341-360
22. Khodorov BI, Revenko SV (1979) Further analysis of the mechanisms of action of batrachotoxin on the membrane of myelinated nerve. Neuroscience 4: 1315-1330
23. Little MJ, Nicholson GM, Zappia C, Gordon D, Martin-Eauclaire M-F, Tyler MI (1997) Australian funnel-web spider toxins and α-scorpion toxins share a common binding site on cockroach and rat voltage-gated sodium channels. Proceedings of the 12th World Congress on Animal, Plant and Microbial Toxins, Cuernavaca, Mexico, September 21-26
24. Lund AE, Narahashi T (1981) Interaction of DDT with sodium channels in squid giant axon membranes. Neuroscience 6: 2253-2258
25. Lund AE, Narahashi T (1981) Kinetics of sodium channel modification by the insecticide tetramethrin in squid giant axon membranes. J Pharmacol Exp Ther 219: 464-473
26. Mozhayeva GN, Naumov AP, Negulyaev YA, Nosyreva ED (1977) The permeability of aconitine-modified sodium channels to univalent cations in myelinated nerve. Biochim Biophys Acta 466: 461-473
27. Mylecharane EJ, Spence I, Sheumack DD, Claassens R, Howden MEH (1989) Actions of robustoxin, a neurotoxic polypeptide from the venom of the male tunnel-web spider (Atrax robustus), in anaesthetized monkeys. Toxicon 27: 481-492
28. Narahashi T, Shapiro BI, Deguchi T, Scuka M, Wang CM (1972) Effects of scorpion venom on squid giant axon membranes. Am J Physiol 222: 850-857
29. Nicholson GM, Willow M, Howden MEH, Narahashi T (1994) Modification of sodium channel gating and kinetics by versutoxin from the Australian funnel-web spider Hadronyche versuta. Pflügers Arch 428: 400-409
30. Nicholson GM, Little MJ, Tyler MI, Narahashi T (1996) Selective alteration of sodium channel gating by Australian funnel-web spider toxins. Toxicon 34:1443-1453
31. Ogata N, Nishimura M, Narahashi T (1989) Kinetics of chlorpromazine block of sodium channels in single guinea pig cardiac myocytes. J Pharmacol Exp Ther 248: 605-613
32. Phillips CA, Spence I, Mylecharane EJ, Brown MR, Sheumack DD, Claassens R, Howden MEH (1987) The effects of the venom from the Blue Mountains funnel-web spider (Atrax versutus) in anaesthetized monkeys. Clin Exp Pharmacol Physiol Suppl 10: 14-15
33. Roy ML, Narahashi T (1992) Differential properties of tetrodotoxin-sensitive and tetrodotoxin-resistant sodium channels in rat dorsal root ganglion neurons. J Neurosci 12: 2104-2111
34. Seyama I, Narahashi T (1981) Modulation of sodium channels of squid nerve membranes by grayanotoxin I. J Pharmacol Exp Ther 219: 614-624
35. Sheridan RE, Adler M (1989) The action of a red tide toxin from Ptychodiscus brevis on single sodium channels in mammalian neuroblastoma cells. FEBS Lett 247: 448-452
36. Sheumack DD, Baldo BA, Carrol PR, Hampson F, Howden MEH, Skorulis A (1984) A comparative study of properties and toxic constituents of funnel-web spider (Atrax) venoms. Comp Biochem Physiol 78°C: 55-68
37. Sheumack DD, Claassens R, Whiteley NM, Howden MEH (1985) Complete amino acid sequence of a new type of lethal neurotoxin from the venom of the funnel-web spider Atrax robustus. FEBS Lett 181: 154-156
38. Spence I, Adams DJ, Gage PW (1977) Funnel-web spider venom produces spontaneous action potentials in nerve. Life Sci 20: 243-250
39. Strichartz GR, Wang GK (1986) Rapid voltage-dependent dissociation of scorpion α-toxins coupled to Na channel inactivation in amphibian myelinated nerves. J Gen Physiol 88: 413-435
40. Sutherland S (1983) Australian animal toxins. Oxford University Press, Melbourne, pp 222-298
41. Tatebayashi H, Narahashi T (1994) Differential mechanism of action of the pyrethroid tetramethrin on tetrodotoxin-sensitive and tetrodotoxin-resistant sodium channels. J Pharmacol Exp Ther 270: 596-603
42. Wang CM, Narahashi T, Scuka M (1972) Mechanism of negative temperature coefficient of nerve blocking action of allethrin. J Pharmacol Exp Ther 182: 442-453
43. Wang GK, Strichartz GR (1983) Purification and physiological characterization of neurotoxins from the venoms of the scorpions Centruroides sculpturatus and Leiurus quinquestriatus. Mol Pharmacol 23: 519-533
44. Wang GK, Strichartz GR (1985) Kinetic analysis of the action of Leiurus scorpion α-toxin on ionic currents in myelinated nerve. J Gen Physiol 86: 739-762
45. Warashina A, Fujita S (1983) Effects of sea anemone toxins on the sodium inactivation process in crayfish axons. J Gen Physiol 81: 305-323
46. + channels by anthopleurin-A: effects on gating and kinetics. Pflügers Arch 424: 15-24
47. Willow M, Gonoi T, Catterall WA (1985). Voltage-clamp analysis of the inhibitory actions of diphenylhydantoin and carbamazepine on voltage-sensitive sodium channels in neuroblastoma cells. Mol Pharmacol 27: 549-558