Three-phase partitioning of α-galactosidase from fermented media of Aspergillus oryzae and comparison with conventional purification techniques

Journal of Industrial Microbiology and Biotechnology

Volumn 36, Issue 1, 2009, Pages 123-128

  Dhananjay, S K ^a   Mulimani, V H ^a  

^a GULBARGA UNIVERSITY   (India)

Author keywords

Galactosidase; Conventional purification; Non aqueous enzymology; T Butanol; Three phase partitioning

Indexed keywords

2 PROPANOL; ALPHA GALACTOSIDASE; AMMONIUM SULFATE; BUTANOL; ORGANIC SOLVENT;

AQUEOUS SOLUTION; ARTICLE; ASPERGILLUS ORYZAE; CHROMATOGRAPHY; CONCENTRATION (PARAMETERS); CULTURE MEDIUM; ENZYME PURIFICATION; FERMENTATION; MOLECULAR WEIGHT; NONHUMAN; PHASE PARTITIONING; POLYACRYLAMIDE GEL ELECTROPHORESIS;

ALPHA-GALACTOSIDASE; AMMONIUM SULFATE; ASPERGILLUS ORYZAE; CHEMICAL FRACTIONATION; CHROMATOGRAPHY; CULTURE MEDIA; FATTY ALCOHOLS; FERMENTATION; FRACTIONAL PRECIPITATION; FUNGAL PROTEINS;

ASPERGILLUS ORYZAE;

EID: 57649149329     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-008-0479-6     Document Type: Article

References (27)

1. A Sadana A Beelaram 1994 Efficiency and economics of bioseparation: some case studies Bioseparation 4 221 235
2. I Roy MN Gupta 2000 Current trends in affinity base separation of proteins/enzymes Curr Sci 78 587 591
3. C Dennison R Lovrein 1997 Three phase-partitioning: concentration and purification of proteins Protein Expr Purif 11 149 161 10.1006/prep.1997.0779
4. L Saxena BK Iyer L Ananthanarayan 2007 Three phase-partitioning as a novel method for purification of ragi (Eleusine coracana) bifunctional amylase/protease inhibitor Process Biochem 42 491 595 10.1016/j.procbio.2006.09. 016
5. A Sharma MN Gupta 2001 Purification of pectinases by three-phase partitioning Biotechnol Lett 23 625 1627 10.1023/A:1011984517432
6. PM Dey JB Pridham 1972 Biochemistry of α-galactosidase Adv Enzymol 36 91 130
7. IV Ulezlo OM Zaprometova 1982 Microbial α-Galactosidase (a review) Prikl Biokhim Mikrobiol 18 3 15
8. Mulimani VH, Ramalingam (1995) Enzymic hydrolysis of raffinose and stachyose present in soymilk by crude α-galactosidase from Gibberella fujikuroi. Biochem Molecul Biol Intern 36:897-905
9. K Girigouda SJ Prashanth VH Mulimani 2005 Oligosaccharides of black gram (Vigna Munga) as effected by processing methods Plant Foods Hum Nutr 60 173 180 10.1007/s11130-005-9552-3
10. RK Morton 1950 Separation and purification of enzymes associated with insoluble particles Nature 166 1092 1095 10.1038/1661092a0
11. RN Pike C Dennison 1989 Protein fractionation by three phase-partitioning (TPP) in aqueous t-butanol mixtures Biotechnol Bioeng 33 221 228 10.1002/bit.260330213
12. SJ Prashanth VH Mulimani 2005 Soymilk oligosaccharides hydrolysis by Aspergillus oryzae α-galactosidase immobilized in calcium alginate Process Biochem 40 1199 1205 10.1016/j.procbio.2004.04.011
13. DS Kulkarni SS Kapanoor K Girigouda NV Kote VH Mulimani 2006 Reduction of flatus inducing factors in soy milk Biotechnol Appl Biochem 45 51 57 10.1042/BA20060027
14. A Sharma S Sharma MN Gupta 2000 Purification of alkaline phosphatase from chicken intestine by three-phase partitioning and use of phenyl-Sepharose 6B in the batch mode Bioseparation 9 155 161 10.1023/A:1008195729472
15. AH Lowry NJ Rosebrough AL Far J Randall 1950 Protein measurement with the phenol reagent J Biol Chem 193 265
16. UK Laemmli 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 680 685 10.1038/227680a0
17. G Ramalingam VH Mulimani 2004 Purification and characterization of guar galactomannann degrading α-galactosidase form Aspergillus oryzae DR-5 J Microbiol Biotechnol 11 863 867
18. SK Kotwal MM Gote IM Khan JM Khire 1999 Production purification and characterization of a constitutive intracellular α-galactosidase form the thermophilic fungus. Hemicola sp J Ind Microbiol Biotechnol 23 661 667 10.1038/sj.jim.2900680
19. S Mi K Meng Y Wang T Yuan H Luo B Yao 2007 Molecular cloning and characterization of a novel α-galactosidase gene from Penicillium sp F63 CGMCC 1669 and expression in Pichia pastoris Enzyme Microb Technol 1380 1373 1380 10.1016/j.enzmictec.2006.10.017
20. Lovrein RE, Goldensoph C, Andreson P, Odegard B (1987) Three phase partitioning (TPP) via t-butanol: enzyme separation from crudes. In: Burgess R (ed) Protein Purification Micro to Macro. Marcel Dekker, New York, pp 521-533
21. C Dennison L Moolman CS Pillay RE Meinesz 2000 t-Butanol: Nature gift for protein isolation S Afr J Sci 96 159 160
22. J Szamos E Kiss 1995 Interfacial behavior of proteins in three phase partitioning using salt containing water/tert-butanol systems J Colloid Interface Sci 170 290 292 10.1006/jcis.1995.1101
23. S Sharma MN Gupta 2000 Three phase partitioning as a large scale separation method for purification of a wheat germ bifunctional protease/amylase inhibitors Process Biochem 37 193 196 10.1016/S0032-9592(01)00199-6
24. MN Gupta 1992 Enzyme function in organic solvents Eur J Biochem 203 25 32 10.1111/j.1432-1033.1992.tb19823.x
25. RK Singh S Gourinath S Sharma I Roy MN Gupta CH Betzel 2001 Enhancement of enzyme activity through three phase-partitioning: crystal structure of a modified serine protease at 1.5 A° resolution Protein Eng 14 307 313 10.1093/protein/14.5.307
26. I Roy A Sharma MN Gupta 2004 Obtaining higher transesterification rates with subtilisin Carlsberg in nonaqueous media Bioorg Med Chem Lett 14 887 889 10.1016/j.bmcl.2003.12.021
27. S Tanuja ND Srinivas MK Gowthman KSMS Raghavarao 2000 Aqueous two phase extraction coupled with ultrafiltration for purification of amyloglucosidase Bioprocess Eng 23 63 68 10.1007/s004499900123