Comparison of cervicovaginal lavage, cervicovaginal lavage enriched with cervical swab, and vaginal tampon for the detection of HIV-1 RNA and HSV-2 DNA in genital secretions

Journal of Acquired Immune Deficiency Syndromes

Volumn 49, Issue 4, 2008, Pages 406-409

  Delany Moretlwe, Sinéad ^a   Rosas, Raul ^b   Mlaba, Nonkululeko ^a   Clayton, Tim ^b   Akpomiemie, Godspower ^a   LeGoff, Jérôme ^c   Capovilla, Alexio ^a   Bélec, Laurent ^c   Stevens, Wendy ^a   Mayaud, Philippe ^b  

^a UNIVERSITY OF THE WITWATERSRAND   (South Africa)

^b LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

^c UNIVERSITÉ PARIS DESCARTES   (France)

Author keywords

African women; Cervicovaginal lavage; Genital shedding; HIV 1 RNA; HSV 2 DNA

Indexed keywords

VIRUS DNA; VIRUS RNA;

ADULT; ARTICLE; BLOOD ANALYSIS; CLINICAL ARTICLE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; FEMALE; HERPES SIMPLEX VIRUS 2; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; MICROSCOPY; MIXED INFECTION; PERFORMANCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SEMEN ANALYSIS; TAMPON; VAGINA SECRETION; VAGINAL LAVAGE; VIRUS DETECTION;

ADOLESCENT; ADULT; CERVIX UTERI; DNA, VIRAL; FEMALE; GENITALIA, FEMALE; HERPES GENITALIS; HERPESVIRUS 2, HUMAN; HIV INFECTIONS; HIV-1; HUMANS; IRRIGATION; MENSTRUAL HYGIENE PRODUCTS; RNA, VIRAL; SPECIMEN HANDLING; VAGINA; YOUNG ADULT;

EID: 57649123369     PISSN: 15254135     EISSN: None     Source Type: Journal    
DOI: 10.1097/QAI.0b013e31818c7f75     Document Type: Article

References (24)

1. Coombs RW, Reichelderfer PS, Landay AL. Recent observations on HIV type-1 infection in the genital tract of men and women. AIDS. 2003;17:455-480.
2. Legoff J, Bouhlal H, Gresenguet G, et al. Real-time PCR quantification of genital shedding of herpes simplex virus (HSV) and human immunodeficiency virus (HIV) in women coinfected with HSV and HIV. J Clin Microbiol. 2006;44:423-432.
3. Ndjoyi-Mbiguino A, Ozouaki F, Legoff J, et al. Comparison of washing and swabbing procedures for collecting genital fluids to assess cervicovaginal shedding of herpes simplex virus type 2 DNA. J Clin Microbiol. 2003;41:2662-2664.
4. Andreoletti L, Gresenguet G, Chomont N, et al. Comparison of washing and swabbing procedures for collecting genital fluids to assess shedding of human immunodeficiency virus type 1 (HIV-1) RNA in asymptomatic HIV-1-infected women. J Clin Microbiol. 2003;41:449-452.
5. Belec L, Meillet D, Levy M, et al. Dilution assessment of cervicovaginal secretions obtained by vaginal washing for immunological assays. Clin Diagn Lab Immunol. 1995;2:57-61.
6. Hart CE, Lennox JL, Pratt-Palmore M, et al. Correlation of human immunodeficiency virus type 1 RNA levels in blood and the female genital tract. J Infect Dis. 1999;179:871-882.
7. Baron P, Bremer J, Wasserman SS, et al. Detection and quantitation of human immunodeficiency virus type 1 in the female genital tract. The Division of AIDS Treatment Research Initiative 009 Study Group. J Clin Microbiol. 2000;38:3822-3824.
8. Nagot N, Foulongne V, Becquart P, et al. Longitudinal assessment of HIV-1 and HSV-2 shedding in the genital tract of West African women. J Acquir Immune Defic Syndr. 2005;39:632-634.
9. Sturm PD, Connolly C, Khan N, et al. Vaginal tampons as specimen collection device for the molecular diagnosis of non-ulcerative sexually transmitted infections in antenatal clinic attendees. Int J STD AIDS. 2004;15:94-98.
10. Tabrizi SN, Paterson BA, Fairley CK, et al. Comparison of tampon and urine as self-administered methods of specimen collection in the detection of Chlamydia trachomatis, Neisseria gonorrhoeae and Trichomonas vaginalis in women. Int J STD AIDS. 1998;9:347-349.
11. Webber MP, Schoenbaum EE, Farzadegan H, et al. Tampons as a self-administered collection method for the detection and quantification of genital HIV-1. AIDS. 2001;15:1417-1420.
12. Coombs RW, Wright DJ, Reichelderfer PS, et al. Variation of human immunodeficiency virus type 1 viral RNA levels in the female genital tract: implications for applying measurements to individual women. J Infect Dis. 2001;184:1187-1191.
13. John GC, Sheppard H, Mbori-Ngacha D, et al. Comparison of techniques for HIV-1 RNA detection and quantitation in cervicovaginal secretions. J Acquir Immune Defic Syndr. 2001;26:170-175.
14. Ndjoyi-Mbiguino A, Ozouaki F, Legoff J, et al. Comparison of washing and swabbing procedures for collecting genital fluids to assess cervicovaginal shedding of herpes simplex virus type 2 DNA. J Clin Microbiol. 2003;41:2662-2664.
15. WHO. Interim WHO Clinical Staging of HIV/AIDS and HIV/AIDS Case Definitions for Surveillance (African Region). Geneva, Switzerland: World Health Organisation; 2005. WHO/HIV/2005.02.
16. Ashley-Morrow R, Nollkamper J, Robinson NJ, et al. Performance of focus ELISA tests for herpes simplex virus type 1 (HSV-1) and HSV-2 antibodies among women in ten diverse geographical locations. Clin Microbiol Infect. 2004;10:530-536.
17. Stevens W, Horsfield P, Scott LE. Evaluation of the performance of the automated NucliSENS easyMAG and EasyQ systems versus the Roche AmpliPrep-AMPLICOR combination for high-throughput monitoring of human immunodeficiency virus load. J Clin Microbiol. 2007;45:1244-1249.
18. Burrows J, Nitsche A, Bayly B, et al. Detection and subtyping of Herpes simplex virus in clinical samples by LightCycler PCR, enzyme immunoassay and cell culture. BMC Microbiol. 2002;2:12.
19. Pepin J, Fink GD, Khonde N, et al. Improving second-generation surveillance: the biological measure of unprotected intercourse using prostate-specific antigen in vaginal secretions of West African women. J Acquir Immune Defic Syndr. 2006;42:490-493.
20. Cowan FF, Pascoe SJ, Barlow KL, et al. Association of genital shedding of herpes simplex virus type 2 and HIV-1 among sex workers in rural Zimbabwe. AIDS. 2006;20:261-267.
21. Kovacs A, Wasserman SS, Burns D, et al. Determinants of HIV-1 shedding in the genital tract of women. Lancet. 2001;358:1593-1601.
22. John-Stewart GC, Nduati RW, Rousseau CM, et al. Subtype C is associated with increased vaginal shedding of HIV-1. J Infect Dis. 2005;192:492-496.
23. Mbopi-Keou FX, Gresenguet G, Mayaud P, et al. Interactions between herpes simplex virus type 2 and human immunodeficiency virus type 1 infection in African women: opportunities for intervention. J Infect Dis. 2000;182:1090-1096.
24. Mark KE, Wald As, Magaret S, et al. Rapid onset and clearance of genital HSV reactivations in immunocompetent adults: The virus is usually "on". Presented at: 17th International Society for STD Research and 10th International Union Against Sexually Transmitted Infections; 2007; Seattle, WA.