Molecular cloning, overproduction, purification and biochemical characterization of the p39 nsp2 protease domains encoded by three alphaviruses

Protein Expression and Purification

Volumn 64, Issue 1, 2009, Pages 89-97

  Zhang, Di ^a   Tözsér, József ^b   Waugh, David S ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b UNIVERSITY OF DEBRECEN   (Hungary)

Author keywords

Affinity tag; Fusion protein; Maltose binding protein; Nonstructural protein 2; Protease; Semliki forest virus; Sindbis virus; Venezuelan equine encephalitis virus

Indexed keywords

CYSTEINE PROTEINASE; DITHIOTHREITOL; EDETIC ACID; GLYCEROL; HYBRID PROTEIN; MERCAPTOETHANOL; NSP2 PROTEINASE; PHOSPHINE DERIVATIVE; SODIUM CHLORIDE; TRIS(2 CARBOXYETHYL)PHOSPHINE; TRIS(2-CARBOXYETHYL)PHOSPHINE;

ALPHA VIRUS; ARTICLE; CHEMISTRY; CLASSIFICATION; ENZYME SPECIFICITY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR WEIGHT; PH; PROTEIN TERTIARY STRUCTURE; TEMPERATURE;

ALPHAVIRUS; CLONING, MOLECULAR; CYSTEINE ENDOPEPTIDASES; DITHIOTHREITOL; EDETIC ACID; GLYCEROL; HUMANS; HYDROGEN-ION CONCENTRATION; MERCAPTOETHANOL; MOLECULAR WEIGHT; PHOSPHINES; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SODIUM CHLORIDE; SUBSTRATE SPECIFICITY; TEMPERATURE;

ALPHAVIRUS; NICOTIANA TABACUM; SEMLIKI FOREST VIRUS; SINDBIS VIRUS; TOBACCO ETCH VIRUS; TOBACCO VEIN MOTTLING VIRUS; VENEZUELAN EQUINE ENCEPHALITIS VIRUS;

EID: 57649089398     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.10.013     Document Type: Article

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