Characterization of a novel Obg-like ATPase in the protozoan Trypanosoma cruzi

International Journal for Parasitology

Volumn 39, Issue 1, 2009, Pages 49-58

  Gradia, Daniela F ^a   Rau, Karlan ^a   Umaki, Adriana C S ^a   de Souza, Flavia S P ^a   Probst, Christian M ^a,b   Correa, Alejandro ^a   Holetz, Fabíola B ^a   Avila, Andréa R ^a,b   Krieger, Marco A ^a,b   Goldenberg, Samuel ^a,b   Fragoso, Stenio P ^a,b  

^a Instituto de Biologia Molecular do Parana   (Brazil)

^b INSTITUTO OSWALDO CRUZ   (Brazil)

Author keywords

Obg; P loop NTPase; Polysome; Proteasome; Translation; Trypanosoma cruzi; YchF

Indexed keywords

ADENOSINE TRIPHOSPHATASE; GUANINE NUCLEOTIDE BINDING PROTEIN; PROTEIN YCHF; PROTOZOAL PROTEIN; UNCLASSIFIED DRUG;

ENZYME ACTIVITY; HYDROLYSIS; IMMUNOASSAY; PROTEIN; PROTOZOAN;

ARTICLE; CELL GROWTH; CHAGAS DISEASE; CONTROLLED STUDY; EUKARYOTE; GENE SEQUENCE; HYDROLYSIS; IMMUNOPRECIPITATION; LEISHMANIA MAJOR; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYSOME; PROTEIN ANALYSIS; PROTEIN FUNCTION; RIBOSOME SUBUNIT; RNA INTERFERENCE; SEQUENCE ANALYSIS; TRYPANOSOMA BRUCEI; TRYPANOSOMA CRUZI; WESTERN BLOTTING;

ADENOSINE TRIPHOSPHATASES; ANIMALS; BASE SEQUENCE; GENES, PROTOZOAN; GTP PHOSPHOHYDROLASES; GTP-BINDING PROTEINS; MALE; MOLECULAR SEQUENCE DATA; RABBITS; RNA, PROTOZOAN; TRYPANOSOMA BRUCEI BRUCEI; TRYPANOSOMA CRUZI;

BACTERIA (MICROORGANISMS); EUKARYOTA; LEISHMANIA MAJOR; PROTOZOA; TRYPANOSOMA BRUCEI; TRYPANOSOMA CRUZI; TRYPANOSOMATIDAE;

EID: 57549116688     PISSN: 00207519     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijpara.2008.05.019     Document Type: Article

References (38)

1. Ahnn J., March P.E., Takiff H.E., and Inouye M. A GTP-binding protein of Escherichia coli has homology to yeast RAS proteins. Proc. Natl. Acad. Sci. USA 83 (1986) 8849-8853
2. Bourne H.R., Sanders D.A., and McCormick F. The GTPase superfamily: a conserved switch of diverse cell functions. Nature 348 (1990) 125-132
3. Bourne H.R., Sanders D.A., and McCormick F. The GTPase superfamily: conserved structure and molecular mechanisms. Nature 349 (1991) 117-127
4. Brecht M., and Parsons M. Changes in polysome profiles accompany trypanosome development. Mol. Biochem. Parasitol. 97 (1998) 189-198
5. Caldon C.E., and March P.E. Function of the universally conserved bacterial GTPases. Curr. Opin. Microbiol. 6 (2003) 135-139
6. Caldon C.E., Yoong P., and March P.E. Evolution of a molecular switch: universal bacterial GTPases regulate ribosome function. Mol. Microbiol. 41 (2001) 289-297
7. De Souza W. Basic cell biology of Trypanosoma cruzi. Curr. Pharm. Des. 8 (2002) 269-285
8. Gavin A.C., Bosche M., Krause R., Grandi P., Marzioch M., Bauer A., Schultz J., Rick J.M., Michon A.-M., Cruciat C.-M., Remor M., Hofert C., Schelder M., Brajenovic M., Ruffner H., Merino A., Klein K., Hudak M., Dickson D., Rudi T., Gnau V., Bauch A., Bastuck S., Huhse B., Leutwein C., Heurtier M.-A., Copley R.R., Edelmann A., Querfurth E., Rybin V., Drewes G., Raida M., Bouwmeester T., Bork P., Seraphin B., Kuster B., Neubauer G., and Superti-Furga G. Functional organization of the yeast proteome by systematic analysis of protein complexes. Nature 415 (2002) 141-147
9. Guerrero C., Tagwerker C., Kaiser P., and Huang L. An integrated mass spectrometry-based proteomic approach. Mol. Cell. Proteomics 5 (2006) 366-378
10. 2 stimulon in Saccharomyces cerevisiae. J. Biol. Chem. 273 (1998) 22480-22489
11. Huynh T.V., Young R.A., and Davis R.W. Construction and screening cDNA libraries in λgt10 and λgt11. In: Glover D.M. (Ed). DNA Cloning - A Practical Approach vol. I (1985), IRL Press, Oxford, England 49-78
12. Jensen B.C., Wang Q., Kifer C.T., and Parsons M. The NOG1 GTP-binding protein is required for biogenesis of the 60S ribosomal subunit. J. Biol. Chem. 278 (2003) 32204-32211
13. Kallstrom G., Hedges J., and Johnson A. The putative GTPases Nog1p and Lsg1p are required for 60S ribosomal subunit biogenesis and are localized to the nucleus and cytoplasm, respectively. Mol. Cell. Biol. 23 (2003) 4333-4355
14. Koller-Eichhorn R., Marquardt T., Gail R., Wittinghofer A., Kostrewa D., Kutay U., and Kambach C. Human OLA1 defines an ATPase subfamily in the OBG family of GTP-binding proteins. J. Biol. Chem. 282 (2007) 19928-19937
15. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
16. Laird P.W. Trans splicing in trypanosomes-archaism or adaptation?. Trends Genet. 5 (1989) 204-208
17. Lanzetta P.A., Alvarez L.J., Reinach P.S., and Candia O.A. An improved assay for nanomole amounts of inorganic phosphate. Anal. Biochem. 100 (1979) 95-97
18. Leipe D.T., Wolf Y.I., Kooning E.V., and Aravind L. Classification and evolution of P-loop GTPases and related ATPases. J. Mol. Biol. 317 (2002) 41-72
19. Morimoto T., Chin L.P., Hirai T., Asai K., Kobayashi K., Moriya S., and Ogasawara N. Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis. Microbiology 148 (2002) 3539-3552
20. Meier T.I., Peery R.B., McAllister K.A., and Zhao G. Era GTPase of Escherichia coli: binding to 16S rRNA and modulation of GTPase activity by RNA and carbohydrates. Microbiology 146 (2000) 1071-1083
21. Meier T.I., Peery R.B., Jaskunas S.R., and Zhao G. 16S ribosomal RNA is bound to ERA of Streptococcus pneumoniae. J. Bacteriol. 181 (1999) 5242-5249
22. Park J.H., Jensen B.C., Kifer C.T., and Parsons M. A novel nucleolar G-protein conserved in eukaryotes. J. Cell Sci. 114 (2001) 173-185
23. Saraste M., Sibbald P.R., and Wittinghofer A. The P-loop - a common motif in ATP- and GTP-binding proteins. Trends Biochem. Sci. 15 (1990) 430-434
24. Sayed A., Matsuyama S., and Inouye M. Era, an essential Escherichia coli small G-protein, binds to the 30S ribosomal subunit. Biochem. Biophys. Res. Commun. 264 (1999) 51-54
25. Scott J.M., Ju J., Mitchell T., and Haldenwang W.G. The Bacillus subtilis GTP binding protein obg and regulators of the sigma(B) stress response transcription factor cofractionate with ribosomes. J. Bacteriol. 182 (2000) 2771-2777
26. Smith D.B., and Johnson K.S. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione-S-transferase. Gene 67 (1988) 31-40
27. Sprang S.R. G protein, effectors and GAPs: structure and mechanism. Curr. Opin. Struct. Biol. 7 (1977) 849-856
28. Sprang S.R. G-protein mechanisms: Insights from structural analysis. Ann. Rev. Biochem. 66 (1997) 639-678
29. Takai Y., Sasaki T., and Matozaki T. Small GTP-binding proteins. Physiol. Rev. 81 (2001) 153-208
30. Teplyakov A., Obmolova G., Chu S.Y., Toedt J., Eisentein E., Howard A.J., and Gilliland G.L. Crystal structure of the YchF protein reveals binding sites for GTP and nucleic acid. J. Bacteriol. 185 (2003) 4031-4037
31. Trach K., and Hoch J.A. The Bacillus subtilis spoOB stage 0 sporulation operon encodes an essential GTP-binding protein. J. Bacteriol. 171 (1989) 1362-1371
32. Vetter I.R., and Wittinghofer A. The guanine nucleotide-binding switch in three dimensions. Science 294 (2001) 1299-1304
33. Welsh K.M., Trach K.A., Folger C., and Hoch J.A. Bacterial characterization of the essential GTP-binding protein Obg of Bacillus subtilis. J. Bacteriol. 176 (1994) 7161-7168
34. Wickstead B., Ersfeld K., and Gull K. Targeting of a tetracycline-inducible expression system to the transcriptionally silent minichromosomes of Trypanosoma brucei. Mol. Biochem. Parasitol. 125 (2002) 211-216
35. Wirtz E., Leal S., Ochatt C., and Cross G.A. A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei. Mol. Biochem. Parasitol. 99 (1999) 89-101
36. Wolf Y.I., Aravind L., Grishin V., and Koonin E.V. Evolution of aminoacyl-tRNA synthetases - analysis of unique domain architectures and phylogenetic trees reveals a complex history of horizontal gene transfer events. Genome Res. 9 (1999) 689-710
37. Wout P., Pu K., Sullivan S.M., Reese V., Zhou S., Lin B., and Maddock J.R. The Escherichia coli GTPase CgtAE cofractionates with the 50S ribosomal subunit and interacts with SpoT, a ppGpp synthetase/hydrolase. J. Bacteriol. 186 (2004) 5249-5257
38. Yim L., Martínez-Vicente M., Villarroya M., Aguado C., Knecht E., and Armengod M.E. The GTPase activity and C-terminal cysteine of the Escherichia coli MnmE protein are essential for its tRNA modifying function. J. Biol. Chem. 278 (2003) 28378-28387