In vitro activity of the antifungal plant defensin RsAFP2 against Candida isolates and its in vivo efficacy in prophylactic murine models of candidiasis

Antimicrobial Agents and Chemotherapy

Volumn 52, Issue 12, 2008, Pages 4522-4525

  Tavares, Patricia M ^a   Thevissen, Karin ^b   Cammue, Bruno P A ^b   François, Isabelle E J A ^b   Barreto Bergter, Eliana ^a   Taborda, Carlos P ^c   Marques, Alexandre F ^c,d   Rodrigues, Marcio L ^a   Nimrichter, Leonardo ^a  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^b UNIVERSITY OF LEUVEN   (Belgium)

^c UNIVERSITY OF SÃO PAULO   (Brazil)

^d UNIVERSITY OF TEXAS AT EL PASO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIFUNGAL AGENT; GLUCOSYLCERAMIDE; RAPHANUS SATIVUS ANTIFUNGAL PEPTIDE 2; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIBIOTIC PROPHYLAXIS; ANTIFUNGAL ACTIVITY; ANTIFUNGAL SUSCEPTIBILITY; ARTICLE; CANDIDA ALBICANS; CANDIDA DUBLINIENSIS; CANDIDA GLABRATA; CANDIDA KRUSEI; CANDIDA PARAPSILOSIS; CANDIDA TROPICALIS; CANDIDIASIS; CONTROLLED STUDY; DISEASE MODEL; DRUG EFFICACY; DRUG SENSITIVITY; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; RADISH;

ANIMALS; ANTIFUNGAL AGENTS; CANDIDA; CANDIDA ALBICANS; CANDIDIASIS; DEFENSINS; GLUCOSYLCERAMIDES; HUMANS; MICE; MICROBIAL SENSITIVITY TESTS; PLANT PROTEINS; TREATMENT OUTCOME;

EID: 57049088716     PISSN: 00664804     EISSN: 10986596     Source Type: Journal    
DOI: 10.1128/AAC.00448-08     Document Type: Article

References (27)

1. Aerts, A. M., I. E. Francois, E. M. Meert, Q. T. Li, B. P. Cammue, and K. Thevissen. 2007. The antifungal activity of RsAFP2, a plant defensin from Raphanus sativus, involves the induction of reactive oxygen species in Candida albicans. J. Mol. Microbiol. Biotechnol. 13:243-247.
2. Barbosa, F. M., S. Daffre, R. A. Maldonado, A. Miranda, L. Nimrichter, and M. L. Rodrigues. 2007. Gomesin, a peptide produced by the spider Acanthoscurria gomesiana, is a potent anticryptococcal agent that acts in synergism with fluconazole. FEMS Microbiol. Lett. 274:279-286.
3. Barns, S. M., D. J. Lane, M. L. Sogin, C. Bibeau, and W. G. Weisburg. 1991. Evolutionary relationships among pathogenic Candida species and relatives. J. Bacteriol. 173:2250-2255.
4. Barreto-Bergter, E., M. R. Pinto, and M. L. Rodrigues. 2004. Structure and biological functions of fungal cerebrosides. An. Acad. Bras. Cienc. 76:67-84.
5. Clemons, K. V., and D. A. Stevens. 2001. Efficacy of the partricin derivative SPA-S-753 against systemic murine candidosis. J. Antimicrob. Chemother. 47:183-186.
6. Collins, B. E., L. J. Yang, and R. L. Schnaar. 2000. Lectin-mediated cell adhesion to immobilized glycosphingolipids. Methods Enzymol. 312:438-446.
7. da Silva, A. F., M. L. Rodrigues, S. E. Farias, I. C. Almeida, M. R. Pinto, and E. Barreto-Bergter. 2004. Glucosylceramides in Colletotrichum gloeosporioides are involved in the differentiation of conidia into mycelial cells. FEBS Lett. 561:137-143.
8. Enoch, D. A., H. A. Ludlam, and N. M. Brown. 2006. Invasive fungal infections: a review of epidemiology and management options. J. Med. Microbiol. 55:809-818.
9. Folch, J., M. Lees, and G. H. Sloane Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497-509.
10. Graybill, J. R., L. K. Najvar, J. D. Holmberg, A. Correa, and M. F. Luther. 1995. Fluconazole treatment of Candida albicans infection in mice: does in vitro susceptibility predict in vivo response? Antimicrob. Agents Chemother. 39:2197-2200.
11. Hendriks, L., A. Goris, Y. Van de Peer, J. M. Neefs, M. Vancanneyt, K. Kersters, G. L. Hennebert, and R. De Wachter. 1991. Phylogenetic analysis of five medically important Candida species as deduced on the basis of small ribosomal subunit RNA sequences. J. Gen. Microbiol. 137:1223-1230.
12. Levery, S. B., M. Momany, R. Lindsey, M. S. Toledo, J. A. Shayman, M. Fuller, K. Brooks, R. L. Doong, A. H. Straus, and H. K. Takahashi. 2002. Disruption of the glucosylceramide biosynthetic pathway in Aspergillus nidulans and Aspergillus fumigatus by inhibitors of UDP-Glc:ceramide glucosyltransferase strongly affects spore germination, cell cycle, and hyphal growth. FEBS Lett. 525:59-64.
13. MacCallum, D. M., and F. C. Odds. 2004. Need for early antifungal treatment confirmed in experimental disseminated Candida albicans infection. Antimicrob. Agents Chemother. 48:4911-4914.
14. Nimrichter, L., E. Barreto-Bergter, R. R. Mendonca-Filho, L. F. Kneipp, M. T. Mazzi, P. Salve, S. E. Farias, R. Wait, C. S. Alviano, and M. L. Rodrigues. 2004. A monoclonal antibody to glucosylceramide inhibits the growth of Fonsecaea pedrosoi and enhances the antifungal action of mouse macrophages. Microbes Infect. 6:657-665.
15. Nimrichter, L., M. D. Cerqueira, E. A. Leitao, K. Miranda, E. S. Nakayasu, S. R. Almeida, I. C. Almeida, C. S. Alviano, E. Barreto-Bergter, and M. L. Rodrigues. 2005. Structure, cellular distribution, antigenicity, and biological functions of Fonsecaea pedrosoi ceramide monohexosides. Infect. Immun. 73:7860-7868.
16. Okawa, Y., M. Miyauchi, S. Takahashi, and H. Kobayashi. 2007. Comparison of pathogenicity of various Candida albicans and C. stellatoidea strains. Biol. Pharm. Bull. 30:1870-1873.
17. Pinto, M. R., M. L. Rodrigues, L. R. Travassos, R. M. Haido, R. Wait, and E. Barreto-Bergter. 2002. Characterization of glucosylceramides in Pseudallescheria boydii and their involvement in fungal differentiation. Glycobiology 12:251-260.
18. Ramamoorthy, V., E. B. Cahoon, J. Li, M. Thokala, R. E. Minto, and D. M. Shah. 2007. Glucosylceramide synthase is essential for alfalfa defensin-mediated growth inhibition but not for pathogenicity of Fusarium graminearum. Mol. Microbiol. 66:771-786.
19. Rittershaus, P. C., T. B. Kechichian, J. C. Allegood, A. H. Merrill, Jr., M. Hennig, C. Luberto, and M. Del Poeta. 2006. Glucosylceramide synthase is an essential regulator of pathogenicity of Cryptococcus neoformans. J. Clin. Investig. 116:1651-1659.
20. Rodrigues, M. L., L. Shi, E. Barreto-Bergter, L. Nimrichter, S. E. Farias, E. G. Rodrigues, L. R. Travassos, and J. D. Nosanchuk. 2007. Monoclonal antibody to fungal glucosylceramide protects mice against lethal Cryptococcus neoformans infection. Clin. Vaccine Immunol. 14:1372-1376.
21. Rodrigues, M. L., L. R. Travassos, K. R. Miranda, A. J. Franzen, S. Rozental, W. de Souza, C. S. Alviano, and E. Barreto-Bergter. 2000. Human antibodies against a purified glucosylceramide from Cryptococcus neoformans inhibit cell budding and fungal growth. Infect. Immun. 68:7049-7060.
22. Rogers, T. R. 2006. Antifungal drug resistance: limited data, dramatic impact? Int. J. Antimicrob. Agents 27(Suppl. 1):7-11.
23. Saito, K., N. Takakuwa, M. Ohnishi, and Y. Oda. 2006. Presence of glucosylceramide in yeast and its relation to alkali tolerance of yeast. Appl. Microbiol. Biotechnol. 71:515-521.
24. Schnaar, R. L., and L. K. Needham. 1994. Thin-layer chromatography of glycosphingolipids. Methods Enzymol. 230:371-389.
25. Terras, F. R., H. M. Schoofs, M. F. De Bolle, F. Van Leuven, S. B. Rees, J. Vanderleyden, B. P. Cammue, and W. F. Broekaert. 1992. Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds. J. Biol. Chem. 267:15301-15309.
26. Thevissen, K., H. H. Kristensen, B. P. Thomma, B. P. Cammue, and I. E. Francois. 2007. Therapeutic potential of antifungal plant and insect defensins. Drug Discov. Today 12:966-971.
27. Thevissen, K., D. C. Warnecke, I. E. Francois, M. Leipelt, E. Heinz, C. Ott, U. Zahringer, B. P. Thomma, K. K. Ferket, and B. P. Cammue. 2004. Defensins from insects and plants interact with fungal glucosylceramides. J. Biol. Chem. 279:3900-3905.