The antifungal activity of RsAFP2, a plant defensin from Raphanus sativus, involves the induction of reactive oxygen species in Candida albicans

Journal of Molecular Microbiology and Biotechnology

Volumn 13, Issue 4, 2007, Pages 243-247

  Aerts, An M ^a   François, Isabelle E J A ^a   Meert, Els M K ^a   Li, Qiu Tian ^b   Cammue, Bruno P A ^a   Thevissen, Karin ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Ascorbic acid; Candida albicans; Glucosylceramide; Raphanus sativus antifungal plant defensin; Reactive oxygen species; Small unilamellar vesicle

Indexed keywords

ANTIFUNGAL AGENT; ASCORBIC ACID; RAPHANUS SATIVUS ANTIFUNGAL PEPTIDE 2; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG;

ANTIFUNGAL ACTIVITY; BINDING SITE; CANDIDA ALBICANS; CELL DEATH; CELL GROWTH; CONFERENCE PAPER; CONTROLLED STUDY; DOSE RESPONSE; DRUG BINDING; NONHUMAN; RADISH; YEAST CELL;

ANTIFUNGAL AGENTS; ASCORBIC ACID; CANDIDA ALBICANS; DEFENSINS; GLUCOSYLCERAMIDES; PERMEABILITY; PLANT PROTEINS; RAPHANUS; REACTIVE OXYGEN SPECIES;

CANDIDA ALBICANS; FUNGI; RAPHANUS SATIVUS;

EID: 34548511597     PISSN: 14641801     EISSN: None     Source Type: Journal    
DOI: 10.1159/000104753     Document Type: Conference Paper

References (28)

1. Lay FT, Anderson MA: Defensins, components of the innate immune system in plants. Curr Prot Pept Sci 2005;6:85-101.
2. Thevissen K, Francois IE, Aerts AM, Cammue BP: Fungal sphingolipids as targets for the development of selective antifungal therapeutics. Curr Drug Targets 2005;6:923-928.
3. Terras FR, Schoofs HM, De Bolle MF, Van Leuven F, Rees SB, Vanderleyden J, Cammue BP, Broekaert WF: Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds. J Biol Chem 1992;267:15301-15309.
4. Thevissen K, Warnecke DC, François IE, Leipelt M, Heinz E, Ott C, Zahringer U, Thomma BP, Ferket KK, Cammue BP: Defensins from insects and plants interact with fungal glucosylceramides. J Biol Chem 2004;279:3900-3905.
5. Thevissen K, Ghazi A, De Samblanx GW, Brownlee C, Osborn RW, Broekaert WF: Fungal membrane responses induced by plant defensins and thionins. J Biol Chem 1996;271:15018-15025.
6. Thevissen K, Terras FR, Broekaert WF: Permeabilization of fungal membranes by plant defensins inhibits fungal growth. Appl Environ Microbiol 1999;65:5451-5458.
7. Leipelt M, Warnecke D, Zahringer U, Ott C, Muller F, Hube B, Heinz E: Glucosylceramide synthases, a gene family responsible for the biosynthesis of glucosphingolipids in animals, plants, and fungi. J Biol Chem 2001;276:33621-33629.
8. Park C, Bennion B, Francois IE, Ferket KK, Cammue BP, Thevissen K, Levery SB: Neutral glycolipids of the filamentous fungus Neurospora crassa: altered expression in plant defensin-resistant mutants. J Lipid Res 2005;46:759-768.
9. Ferket KK, Levery SB, Park C, Cammue BP, Thevissen K: Isolation and characterization of Neurospora crassa mutants resistant to antifungal plant defensins. Fungal Genet Biol 2003;40:176-185.
10. Sakaki T, Zahringer U, Warnecke DC, Fahl A, Knogge W, Heinz E: Sterol glycosides and cerebrosides accumulate in Pichia pastoris , Rhynchosporium secalis and other fungi under normal conditions or under heat shock and ethanol stress. Yeast 2001;18:679-695.
11. Valcarcel CA, Dalla Serra M, Potrich C, Bernhart I, Tejuca M, Martinez D, Pazos F, Lanio ME, Menestrina G: Effects of lipid composition on membrane permeabilization by sticholysin I and II, two cytolysins of the sea anemone Stichodactyla helianthus. Biophys J 2001;80:2761-2774.
12. Zhao H, Mattila JP, Holopainen JM, Kinnunen PK: Comparison of the membrane association of two antimicrobial peptides, magainin 2 and indolicidin. Biophys J 2001;81:2979-2991.
13. Therade-Matharan S, Laemmel E, Carpentier S, Obata Y, Levade T, Duranteau J, Vicaut E: Reactive oxygen species production by mitochondria in endothelial cells exposed to reoxygenation after hypoxia and glucose depletion is mediated by ceramide. Am J Physiol 2005;289:R1756-R1762.
14. Moussavi M, Assi K, Gomez-Munoz A, Salh B: Curcumin mediates ceramide generation via the de novo pathway in colon cancer cells. Carcinogenesis 2006;27:1636-1644.
15. Ames BN, Shigenaga MK, Hagen TM: Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci USA 1993;90:7915-7922.
16. François IEJA, Cammue BPA, Borgers M, Ausma J, Dispersyn GD, Thevissen K: Azoles: mode of antifungal action and resistance development. Effect of miconazole on endogenous reactive oxygen species production in Candida albicans. Anti-Infect Agents Med Chem 2006;5:3-13.
17. Kobayashi D, Kondo K, Uehara N, Otokozawa S, Tsuji N, Yagihashi A, Watanabe N: Endogenous reactive oxygen species is an important mediator of miconazole antifungal effect. Antimicrob Agents Chemother 2002;46:3113-3117.
18. Phillips AJ, Sudbery I, Ramsdale M: Apoptosis induced by environmental stresses and amphotericin B in Candida albicans. Proc Natl Acad Sci USA 2003;100:14327-14332.
19. Nakayama K, Yamaguchi T, Doi T, Usuki Y, Taniguchi M, Tanaka T: Synergistic combination of direct plasma membrane damage and oxidative stress as a cause of antifungal activity of polyol macrolide antibiotic niphimycin. J Biosci Bioeng 2002;94:207-211.
20. Hiramoto F, Nomura N, Furumai T, Oki T, Igarashi Y: Apoptosis-like cell death of Saccharomyces cerevisiae induced by a mannose-binding antifungal antibiotic, pradimicin. J Antibiot (Tokyo) 2003;56:768-772.
21. Xing MZ, Zhang XZ, Sun ZL, Zhang HY: Perylenequinones act as broad-spectrum fungicides by generating reactive oxygen species both in the dark and in the light. J Agric Food Chem 2003;51:7722-7724.
22. Machida K, Tanaka T, Fujita K, Taniguchi M: Farnesol-induced generation of reactive oxygen species via indirect inhibition of the mitochondrial electron transport chain in the yeast Saccharomyces cerevisiae. J Bacteriol 1998;180:4460-4465.
23. Narasimhan ML, Damsz B, Coca MA, Ibeas JI, Yun DJ, Pardo JM, Hasegawa PM, Bressan RA: A plant defense response effector induces microbial apoptosis. Mol Cell 2001;8:921-930.
24. Leiter E, Szappanos H, Oberparleiter C, Kaiserer L, Csernoch L, Pusztahelyi T, Emri T, Pocsi I, Salvenmoser W, Marx F: Antifungal protein PAF severely affects the integrity of the plasma membrane of Aspergillus nidulans and induces an apoptosis-like phenotype. Antimicrob Agents Chemother 2005;49:2445-2453.
25. Vorland LH: Lactoferrin: a multifunctional glycoprotein. APMIS 1999;107:971-981.
26. Madeo F, Fröhlich E, Ligr M, Grey M, Sigrist SJ, Wolf DH, Fröhlich K-U: Oxygen stress: a regulator for apoptosis in yeast. J Cell Biol 1999;145:757-767.
27. Fonzi WA, Irwin MY: Isogenic strain construction and gene mapping in Candida albicans. Genetics 1993;134:717-728.
28. Thulborn KR, Sawyer WH: Properties and the locations of a set of fluorescent probes sensitive to the fluidity gradient of the lipid bilayer. Biochim Biophys Acta 1978;511:125-140.