Significance of stereochemistry of 3′-terminal phosphorothioate- modified primer in DNA polymerase-mediated chain extension

Molecular Biotechnology

Volumn 40, Issue 2, 2008, Pages 119-126

  Nawrot, Barbara ^a   Paul, Natasha ^b   Rebowska, Beata ^a   Stec, Wojciech J ^a  

^a CENTRE OF MOLECULAR AND MACROMOLECULAR STUDIES   (Poland)

^b TriLink BioTechnologies Inc   (United States)

Author keywords

Chemical synthesis; Diastereomers; DNA polymerase; Exonuclease; Oxathiaphospholane synthesis; P chirality; PCR; Phosphorothioate; Primer; PS oligonucleotide; Single base extension; Stereochemistry; Stereocontrolled synthesis

Indexed keywords

ABS RESINS; DNA; GENES; ION EXCHANGE; MIXTURES; NUCLEIC ACIDS; OLIGONUCLEOTIDES; ORGANIC ACIDS; PORT TERMINALS; PRECIPITATION (CHEMICAL); STEREOCHEMISTRY;

CHEMICAL SYNTHESIS; DIASTEREOMERS; DNA POLYMERASE; EXONUCLEASE; OXATHIAPHOSPHOLANE SYNTHESIS; P-CHIRALITY; PCR; PHOSPHOROTHIOATE; PRIMER; PS-OLIGONUCLEOTIDE; SINGLE BASE EXTENSION; STEREOCONTROLLED SYNTHESIS;

SYNTHESIS (CHEMICAL);

2' DEOXYNUCLEOSIDE; DNA POLYMERASE; OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATE;

ARTICLE; CHIRALITY; DIASTEREOISOMER; DNA SYNTHESIS; ION EXCHANGE CHROMATOGRAPHY; NUCLEOTIDE SEQUENCE; REACTION ANALYSIS; STEREOCHEMISTRY; STEREOSPECIFICITY;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA PRIMERS; DNA-DIRECTED DNA POLYMERASE; MOLECULAR STRUCTURE; PHOSPHOROTHIOATE OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; STEREOISOMERISM;

EID: 56749164916     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-008-9096-x     Document Type: Article

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