Stability of stereoregular oligo(nucleoside phosphorothioate)s in human plasma: Diastereoselectivity of plasma 3'-exonuclease

Antisense and Nucleic Acid Drug Development

Volumn 7, Issue 1, 1997, Pages 43-48

  KozioŁkiewicz, Maria ^a   Wójcik, Marzena ^a   Kobylańska, Anna ^a   Karwowski, BolesŁaw ^a   Rȩbowska, Beata ^a   Guga, Piotr ^a   Stec, Wojciech J ^a  

^a CENTRE OF MOLECULAR AND MACROMOLECULAR STUDIES   (Poland)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYCYTIDINE; EXONUCLEASE; NUCLEIC ACID BASE; OLIGONUCLEOTIDE;

ARTICLE; DIASTEREOISOMER; ENZYME ACTIVITY; ENZYME BLOOD LEVEL; HUMAN; MOLECULAR STABILITY; PRIORITY JOURNAL; STEREOCHEMISTRY;

EID: 0030980028     PISSN: 10872906     EISSN: None     Source Type: Journal    
DOI: 10.1089/oli.1.1997.7.43     Document Type: Article

References (6)

1. BURGERS, P.M., and ECKSTEIN, F. (1979). A study of the mechanism of DNA polymerase I from Escherichia coli with diastereomeric phosphorothioate analogs of deoxyadenosine triphosphate. J. Biol. Chem. 254, 6889-6893.
2. ECKSTEIN, F. (1985). Nucleoside phosphorothioates. Annu. Rev. Biochem. 54, 367-402.
3. EDER, P.S., DEVINE, R.J., DAGLE, J.M., and WALDER, J.A. (1991) Substrate specificity and kinetics of degradation of antisense oligonucleotides by a 3′ exonuclease in plasma. Antisense Res. Dev. 1, 141-151.
4. GERLT, J.A., CODERRE, J.A., and MEHDI, S. (1983). Oxygen chiral phosphate esters. Adv. Enzymol. 55, 291-380.
5. GRIFFITHS, A.D., POTTER, B.V.L., and EPERON, I.C. (1987). Stereospecificity of nucleases towards phosphorothioate-substituted RNA: Stereochemistry of transcription by T7 RNA polymerase. Nucleic Acids Res. 15, 4145-4162.
6. ZON, G., and STEC, W.J. (1991). Phosphorothioate oligonucleotides. In Oligonucleotides and Analogues: A Practical Approach. F. Eckstein, (IRL Press, Oxford), pp. 87-108.