Recombinant expression of aryl hydrocarbon receptor for quantitative ligand-binding analysis

Analytical Biochemistry

Volumn 384, Issue 2, 2009, Pages 279-287

  Fan, Ming Qi ^a   Bell, Alex R ^b   Bell, David R ^a   Clode, Sally ^c   Fernandes, Alwyn ^d   Foster, Paul M D ^e   Fry, Jeffrey R ^f   Jiang, Tao ^a   Loizou, George ^g   MacNicoll, Alan ^d   Miller, Brian G ^h   Rose, Martin ^d   Shaikh Omar, Osama ^a   Tran, Lang ^h   White, Shaun ^d  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^b ASTRAZENECA   (United Kingdom)

^c Department of Environmental Air Quality   (United Kingdom)

^d CENTRAL SCIENCE LABORATORY   (United Kingdom)

^e NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES   (United States)

^f UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^g HEALTH AND SAFETY LABORATORY   (United Kingdom)

^h INSTITUTE OF OCCUPATIONAL MEDICINE   (United Kingdom)

Author keywords

Aryl hydrocarbon receptor; Dioxin; Ligand binding; PCB; Species difference

Indexed keywords

BINDING ENERGY; HYDROCARBONS; ORGANIC POLLUTANTS; POLYCHLORINATED BIPHENYLS; PROTEINS; RATS;

ARYL HYDROCARBON RECEPTOR; DIOXIN; LIGAND BINDING; LIGAND BINDING AFFINITY; LIGAND BINDING CAPACITY; PROTEIN CONCENTRATIONS; QUANTITATIVE ESTIMATES; SPECIES DIFFERENCE;

LIGANDS;

2,3,7,8 TETRACHLORODIBENZO PARA DIOXIN; AROMATIC HYDROCARBON RECEPTOR; CHAPERONE; PROTEIN P23; RECOMBINANT RECEPTOR;

ANIMAL CELL; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; BINDING AFFINITY; BINDING ASSAY; CONCENTRATION RESPONSE; CONTROLLED STUDY; HUMAN; INSECT CELL; LEPIDOPTERA; LIGAND BINDING; LIVER CYTOSOL; NONHUMAN; NUCLEOTIDE SEQUENCE; PARAMETER; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; RAT; RECEPTOR AFFINITY; REPRODUCIBILITY; SPECIES DIFFERENCE; TECHNIQUE;

RATTUS; SPODOPTERA FRUGIPERDA;

EID: 56749098105     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.10.003     Document Type: Article

References (36)

1. Poland A., Glover E., and Kende A.S. Stereospecific high affinity binding of 2, 3, 7, 8-tetrachlorodibenzo-para-dioxin by hepatic cytosol-evidence that binding species is receptor for induction of aryl-hydrocarbon hydroxylase. J. Biol. Chem. 251 (1976) 4936-4946
2. Poland A., and Knutson J.C. 2, 3, 7, 8-Tetrachlorodibenzo-para-dioxin and related halogenated aromatic-hydrocarbons-examination of the mechanism of toxicity. Annu. Rev. Pharmacol. Toxicol. 22 (1982) 517-554
3. Burbach K.M., Poland A., and Bradfield C.A. Cloning of the Ah-receptor Cdna reveals a distinctive ligand-activated transcription factor. Proc. Natl. Acad. Sci. USA 89 (1992) 8185-8189
4. Ema M., Sogawa K., Watanabe N., Chujoh Y., Matsushita N., Gotoh O., et al. Cdna cloning and structure of mouse putative Ah receptor. Biochem. Biophys. Res. Commun. 184 (1992) 246-253
5. Fernandezsalguero P., Pineau T., Hilbert D.M., McPhail T., Lee S.S.T., Kimura S., et al. Immune-system impairment and hepatic-fibrosis in mice lacking the dioxin-binding Ah receptor. Science 268 (1995) 722-726
6. FernandezSalguero P.M., Hilbert D.M., Rudikoff S., Ward J.M., and Gonzalez F.J. Aryl-hydrocarbon receptor-deficient mice are resistant to 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin-induced toxicity. Toxicol. Appl. Pharmacol. 140 (1996) 173-179
7. Schmidt J.V., Su G.H.T., Reddy J.K., Simon M.C., and Bradfield C.A. Characterization of a murine Ahr null allele: involvement of the Ah receptor in hepatic growth and development. Proc. Natl. Acad. Sci. USA 93 (1996) 6731-6736
8. Shimizu Y., Nakatsuru Y., Ichinose M., Takahashi Y., Kume H., Mimura J., et al. Benzo[a]pyrene carcinogenicity is lost in mice lacking the aryl hydrocarbon receptor. Proc. Natl. Acad. Sci. USA 97 (2000) 779-782
9. Bradfield C.A., Kende A.S., and Poland A. Kinetic and equilibrium studies of Ah receptor-ligand binding-use of (i-125)2-lodo-7, 8-dibromodibenzo-para-dioxin. Mol. Pharmacol. 34 (1988) 229-237
10. Farrell K., and Safe S. Absence of positive cooperativity in the binding of 2, 3, 7, 8-tetrachlorodibenzo-para-dioxin to its cytosolic receptor protein. Biochem. J. 244 (1987) 539-546
11. Bradfield C.A., and Poland A. A Competitive-binding assay for 2, 3, 7, 8-tetrachlorodibenzo-para-dioxin and related ligands of the Ah receptor. Mol. Pharmacol. 34 (1988) 682-688
12. Connor K.T., and Aylward L.L. Human response to dioxin: aryl hydrocarbon receptor (AhR) molecular structure, function, and dose-response data for enzyme induction indicate an impaired human AhR. J. Toxicol. Environ. Health Part B 9 (2006) 147-171
13. Ema M., Ohe N., Suzuki M., Mimura J., Sogawa K., Ikawa S., et al. Dioxin binding activities of polymorphic forms of mouse and human arylhydrocarbon receptors. J. Biol. Chem. 269 (1994) 27337-27343
14. Poland A., Palen D., and Glover E. Analysis of the 4 alleles of the murine aryl-hydrocarbon receptor. Mol. Pharmacol. 46 (1994) 915-921
15. Harper P.A., Golas C.L., and Okey A.B. Characterization of the Ah receptor and aryl-hydrocarbon hydroxylase induction by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin and benz(a)anthracene in the human A431 squamous-cell carcinoma line. Cancer Res. 48 (1988) 2388-2395
16. Manchester D.K., Gordon S.K., Golas C.L., Roberts E.A., and Okey A.B. Ah-receptor in human-placenta-stabilization by molybdate and characterization of binding of 2, 3, 7, 8-tetrachlorodibenzo-para-dioxin, 3-methylcholanthrene, and benzo(a)pyrene. Cancer Res. 47 (1987) 4861-4868
17. 2+-dependent proteolysis of the Ah receptor. Arch. Biochem. Biophys. 261 (1988) 103-111
18. Coumailleau P., Poellinger L., Gustafsson J.A., and Whitelaw M.L. Definition of a minimal domain of the dioxin receptor that is associated with Hsp90 and maintains wild-type ligand-binding affinity and specificity. J. Biol. Chem. 270 (1995) 25291-25300
19. Ramadoss P., and Perdew G.H. Use of 2-azido-3-[I-125]iodo-7, 8-dibromodibenzo-p-dioxin as a probe to determine the relative ligand affinity of human versus mouse aryl hydrocarbon receptor in cultured cells. Mol. Pharmacol. 66 (2004) 129-136
20. Chan W.K., Chu R.Y., Jain S., Reddy J.K., and Bradfield C.A. Baculovirus expression of the Ah receptor and Ah receptor nuclear translocator-evidence for additional dioxin-responsive element-binding species and factors required for signaling. J. Biol. Chem. 269 (1994) 26464-26471
21. Morishima Y., Kanelakis K.C., Murphy P.J.M., Lowe E.R., Jenkins G.J., Osawa Y., et al. The Hsp90 cochaperone p23 is the limiting component of the multiprotein Hsp90/Hsp70-based chaperone system in vivo where it acts to stabilize the client protein center dot Hsp90 complex. J. Biol. Chem. 278 (2003) 48754-48763
22. Holmes J.L., and Pollenz R.S. Determination of aryl hydrocarbon receptor nuclear translocator protein concentration and subcellular localization in hepatic and nonhepatic cell culture lines: development of quantitative Western blotting protocols for calculation of aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator protein in total cell lysates. Mol. Pharmacol. 52 (1997) 202-211
23. Bell D.R., and Poland A. Binding of aryl hydrocarbon receptor (AhR) to AhR-interacting protein-the role of hsp90. J. Biol. Chem. 275 (2000) 36407-36414
24. Yao G., Craven M., Drinkwater N., and Bradfield C.A. Interaction networks in yeast define and enumerate the signaling steps of the vertebrate aryl hydrocarbon receptor. PLoS Biol. 2 (2004) e65
25. Dolwick K.M., Schmidt J.V., Carver L.A., Swanson H.I., and Bradfield C.A. Cloning and expression of a human Ah receptor Cdna. Mol. Pharmacol. 44 (1993) 911-917
26. Cox M.B., and Miller C.A. The p23 co-chaperone facilitates dioxin receptor signaling in a yeast model system. Toxicol. Lett. 129 (2002) 13-21
27. Miller C.A. Two tetratricopeptide repeat proteins facilitate human aryl hydrocarbon receptor signalling in yeast. Cell. Signal. 14 (2002) 615-623
28. Whitelaw M.L., McGuire J., Picard D., Gustafsson J.A., and Poellinger L. Heat-shock protein Hsp90 regulates dioxin receptor function in-vivo. Proc. Natl. Acad. Sci. USA 92 (1995) 4437-4441
29. Shetty P.V., Bhagwat B.Y., and Chan W.K. P23 enhances the formation of the aryl hydrocarbon receptor-DNA complex. Biochem. Pharmacol. 65 (2003) 941-948
30. Pohjanvirta R., Viluksela M., Tuomisto J.T., Unkila M., Karasinska J., Franc M.A., et al. Physicochemical differences in the AH receptors of the most TCDD-susceptible and the most TCDD-resistant rat strains. Toxicol. Appl. Pharmacol. 155 (1999) 82-95
31. Lorenzen A., and Okey A.B. Detection and characterisation of Ah receptor in tissue and cells from human tonsils. Toxicol. Appl. Pharmacol. 107 (1991) 203-214
32. Okey A.B., Giannone J.V., Smart W., Wong J.M.Y., Manchester D.K., Parker N.B., et al. Binding of 2, 3, 7-8-tetrachlorodibenzo-p-dioxin to AH receptor in placentas from normal versus abnormal pregnancy outcomes. Chemosphere 34 (1997) 1535-1547
33. Butler R.A., Kelley M.L., Powell W.H., Hahn M.E., and Van Beneden R.J. An aryl hydrocarbon receptor (AHR) homologue from the soft-shell clam, Mya arenaria: evidence that invertebrate AHR homologues lack 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin and beta-naphthoflavone binding. Gene 278 (2001) 223-234
34. Duncan D.M., Burgess E.A., and Duncan I. Control of distal antennal identity and tarsal development in Drosophila by spineless-aristapedia, a homolog of the mammalian dioxin receptor. Genes Dev. 12 (1998) 1290-1303
35. Powell-Coffman J.A., Bradfield C.A., and Wood W.B. Caenorhabditis elegans orthologs of the aryl hydrocarbon receptor and its heterodimerization partner the aryl hydrocarbon receptor nuclear translocator. Proc. Natl. Acad. Sci. USA 95 (1998) 2844-2849
36. Fernandes A., White S., D'Silva K., and Rose M. Simultaneous determination of PCDDs, PCDFs, PCBs and PBDEs in food. Talanta 63 (2004) 1147-1155