Determination of aryl hydrocarbon receptor nuclear translocator protein concentration and subcellular localization in hepatic and nonhepatic cell culture lines: Development of quantitative western blotting protocols for calculation of aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator protein in total cell lysates

Molecular Pharmacology

Volumn 52, Issue 2, 1997, Pages 202-211

  Holmes, Jennifer L ^a   Pollenz, Richard S ^a  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AROMATIC HYDROCARBON RECEPTOR; CARRIER PROTEIN; NUCLEAR PROTEIN;

ANIMAL CELL; ARTICLE; CELL LYSATE; CONTROLLED STUDY; DIMERIZATION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOCYTOCHEMISTRY; IMMUNOPRECIPITATION; LIVER CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT; REGRESSION ANALYSIS; SIGNAL TRANSDUCTION;

EID: 0030751810     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: 10.1124/mol.52.2.202     Document Type: Article

References (32)

1. Reyes, H., S. Reisz-Porszasz, and O. Hankinson. Identification of the Ah receptor nuclear translocator protein (Arnt) as a component of the DNA binding form of the Ah receptor. Science (Washington D. C.) 256:1193-1195 (1992).
2. Whitlock, J. P., Jr. Genetic and molecular aspects of 2,3,7,8-tetrachlorodibenzo-p-dioxin action. Annu. Rev. Pharmacol. Toxicol. 30:251-277 (1990).
3. Whitlock, J. P. Mechanistic aspects of dioxin action. Chem. Res. Toxicol. 6:754-763 (1993).
4. Hankinson, O. The Aryl hydrocarbon receptor complex. Annu. Rev. Pharm. Toxicol. 35:307-40 (1995).
5. Antonsson, C., V. Arulampalam, M. A. Whitelaw, S. Petersson, and L. Poellinger. Constitutive function of the basic-helix-loop-helix/PAS factor ARNT: regulation of target promoters via the E-box motif. J. Biol. Chem. 270:13968-13972 (1995).
6. Sogawa, K., R. Nakano, A. Kobayashi, Y. Kikuchi, N. Ohe, N. Matsushita, and Y. Fuji-Kuriyama. Possible function of Ah receptor nuclear translocator (ARNT) homodimer in transcriptional regulation. Proc. Natl. Acad. Sci. USA 92:1936-1940 (1995).
7. Swanson, H. I., W. K. Chan, and C. A. Bradfield. DNA binding specificities and pairing rules of the Ah receptor, ARNT, and SIM proteins. J. Biol. Chem. 270:26292-26302 (1996).
8. Li, H., H. P. Ko, and J. P. Whitlock, Jr. Induction of phosphoglycerate kinase 1 gene expression by hypoxia. J. Biol. Chem. 271:21262-21267 (1996).
9. Jiang, B.-H., E. Rue, G. L. Wang, R. Roe, and G. L. Semenza. Dimerization, DNA binding and transactivation properties of hypoxia-inducible factor 1. J. Biol. Chem. 271:17771-17778 (1996).
10. McGuire, J., P. Coumailleau, M. L. Whitelaw, J.-A. Gustafsson, and L. Poellinger. The basic-helix-loop-helix/PAS factor SIM is associated with hsp90. J. Biol. Chem. 270:31353-31357 (1996).
11. Gradin, K., J. McGuire, R. H. Wenger, I. Kvietikova, M. L. Whitelaw, R. Toftgard, L. Tora, M. Gassmann, and L. Poellinger. Functional interference between hypoxia and dioxin signal transduction pathways: competition for recruitment of the Arnt transcription factor. Mol. Cell. Biol. 16: 5221-5321 (1996).
12. 2 tension. Proc. Natl. Acad. Sci. USA 92:5510-5514 (1995).
13. Wood, S. W., J. M. Gleadle, C. W. Pugh, O. Hankinson, and P. J. Ratcliffe. The role of the aryl hydrocarbon receptor nuclear translocator (ARNT) in hypoxic induction of gene expression. J. Biol. Chem. 271:15117-15123 (1996).
14. Hirose, K., M. Morita, M. Ema, J. Mimura, H. Hamada, H. Fuji, Y. Saijo, O. Gotoh, K. Sogawa, and Y. Fuji-Kuriyama. cDNA cloning and tissue-specific expression of a novel bHLH/PAS factor (ARNT2) with close sequence similarity to the aryl hydrocarbon nuclear translocator (ARNT). Mol. Cell. Biol. 16:1706-1713 (1996).
15. Drutel, G., M. Kathmann, A. Heron, J.-C. Schwartz, and J.-M. Arrang. Cloning and selective expression in brain and kidney of ARNT2 homologous to the Ah receptor nuclear translocator (ARNT). Biochem. Biophys. Res. Commun. 225:333-339 (1996).
16. Pollenz, R. S., H. L. Sullivan, J. Holmes, B. Necela, and R. E. Peterson. Isolation and characterization of cDNAs from rainbow trout (Oncorhynchus mykiss) that encode two novel basic-helix-loop-helix/PAS proteins with distinct functions in the presence of the aryl hydrocarbon receptor. J. Biol. Chem. 271:30886-30896 (1996).
17. Maltepe, E., J. V. Schmidt, D. Baunoch, C. A. Bradfield, and M. C. Simon. Abnormal angiogenesis and response to glucose and oxygen deprivation in mice lacking the protein ARNT. Nature (Lond.). 386:403-406 (1997).
18. Pollenz, R. S., C. A. Sattler, and A. Poland. The aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator protein show distinct subcellular localization in Hepa 1c1c7 cell by immunofluorescence microscopy. Mol Pharmacol. 45:428-438 (1994).
19. Pollenz, R. S. The aryl hydrocarbon receptor but not the aryl hydrocarbon receptor nuclear translocator protein is rapidly depleted in hepatic and nonhepatic culture cells exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin. Mol. Pharmacol. 49:391-398 (1996).
20. Pollenz, R. S. Linearity of Western blotting with ECL: quantification of changes in protein concentration in whole cell lysates, in ECL Highlights No. 9 (P. Riis, ed.). Amersham, Arlington Heights, IL, 2-3 (1996).
21. Hein, J. Unified approach to alignment and phylogenies. Methods Enzymol. 183:626-645 (1990).
22. Legraverend, C., R. R. Hannah, H. J. Eisen, I. S. Owens, D. W. Nebert, and O. Hankinson. Regulatory gene product of the Ah locus. J. Biol. Chem. 257:6402-6407 (1982).
23. Lodish, D. Baltimore, A. Berk, S. L. Zipursky, P. Matsudaira, and J. Darnell. Molecular Cell Biology. Scientific American Books, New York (1996).
24. Singh, S. S., N. G. Hord, and G. H. Perdew. Characterization of the activated form of the aryl hydrocarbon receptor in the nucleus of HeLa-1 cells in the absence of exogenous ligand. Arch. Biochem. Biophys. 329: 47-55 (1996).
25. Li, H., L. Dong, and J. P. Whitlock, Jr. Transcriptional activation function of the mouse Ah receptor nuclear translocator. J. Biol. Chem. 269:28094-28105 (1994).
26. Burbach, K. M., A. Poland, and C. A. Bradfield. Cloning of the Ah-receptor cDNA reveals a distinctive ligand activated transcription factor. Proc. Natl. Acad. Sci. USA 89:8185-8189 (1992).
27. Carver, L. A., J. B. Hogenesch, and C. A. Bradfield. Tissue specific expression of the rat Ah-receptor and ARNT mRNAs. Nucleic Acids Res. 22:3038-3044 (1994).
28. Dolwick, K. M., J. V. Schmidt, L. A. Carver, H. I. Swanson, and C. A. Bradfield. Cloning and expression of a human Ah receptor cDNA. Mol. Pharmacol. 44:911-917 (1993).
29. Okey, A. B., G. P. Bondy, M. E. Mason, D. W. Nebert, C. J. Forster-Gibson, J. Muncan, and M. J. Dufresne. Temperature-dependent cytosol-to-nucleus translocation of the Ah receptor for 2,3,7,8-tetrachlorodibenzo-p-dioxin in continuous cell culture lines. J. Biol. Chem. 255:11415-11422 (1980).
30. Hord, H. G. and G. H. Perdew. Physiochemical and immunocytochemical analysis of the aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator: characterization of two monoclonal antibodies to the aryl hydrocarbon receptor nuclear translocator. Mol. Pharmacol. 46:618-626 (1994).
31. Kalderon, D., W. D. Richardson, A. F. Markham, and A. E. Smith, A. E. Sequence requirements for the nuclear localization of simian virus 40 large-T antigen. Nature (Lond.) 311:33-38 (1984).
32. Dingwall, C., and R. A. Laskey. Transport of proteins into the nucleus. Annu. Rev. Cell Biol. 2:367-390 (1986).