Global expression of simulated microgravity-responsive genes in Xenopus liver cells

Zoological Science

Volumn 25, Issue 8, 2008, Pages 828-837

  Ikuzawa, Masayuki ^a   Asashima, Makoto ^a,b,c  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

3D clinostat; A8 cell; Liver; Microarray; Random positioning machine; Simulated microgravity; Xenopus laevis

Indexed keywords

MESSENGER RNA; OSTEONECTIN;

ANIMAL; ARTICLE; CELL CULTURE; CYTOLOGY; DOWN REGULATION; GENE EXPRESSION; GENETICS; KIDNEY; LIVER; METABOLISM; METHODOLOGY; MICROARRAY ANALYSIS; PHYSIOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TIME; WEIGHTLESSNESS; XENOPUS LAEVIS;

ANIMALS; CELLS, CULTURED; DOWN-REGULATION; GENE EXPRESSION; KIDNEY; LIVER; MICROARRAY ANALYSIS; OSTEONECTIN; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TIME FACTORS; WEIGHTLESSNESS; WEIGHTLESSNESS SIMULATION; XENOPUS LAEVIS;

XENOPUS LAEVIS;

EID: 56649100673     PISSN: 02890003     EISSN: 02890003     Source Type: Journal    
DOI: 10.2108/zsj.25.828     Document Type: Article

References (17)

1. Evans GL, Morey-Holton E, Turner RT (1998) Spaceflight has compartment- and gene-specific effects on mRNA levels for bone matrix proteins in rat femur. J Appl Physiol 84: 2132-2137
2. Firts RH, Riley DR, Widrick JJ (2001) Functional and structural adaptations of skeletal muscle to microgravity. J Exp Biol 204: 3201-3208
3. Francki A, Sage EH (2001) SPARC and the kidney glomerulus: matricellular proteins exhibit diverse functions under normal and pathological conditions. Trends Cardiovasc Med 11: 32-37
4. Harris SA, Zhang M, Kidder LS, Evans GL, Spelsberg TC, Turner RT (2000) Effects of orbital spaceflight on human osteoblastic cell physiology and gene expression. Bone 26: 325-331
5. Ichigi J, Asashima M (2001) Dome formation and tubule morphogenesis by Xenopus kidney A6 cell cultures exposed to microgravity. In Vitro Cell Dev Biol Anim 37: 31-44
6. Ikuzawa M, Akiduki S, Asashima M (2007) Gene expression profile of Xenopus A6 cells cultured under random positioning machine shows downregulation of ion transporter genes and inhibition of dome formation. Adv Space Res 40: 1694-1702
7. Iwamoto J, Takeda T, Sato Y (2005) Interventions to prevent bone loss in astronauts during space flight. Keio J Med 54: 55-59
8. Kitamoto J, Fukui A, Asashima M (2005) Temporal regulation of global gene expression and cellular morphology in Xenopus kidney cells in response to clinorotation. Adv Space Res 35: 1654-1661
9. Kyuno J, Fukui A, Michiue T, Asashima M (2003) Characterization of a gene respondent to clinorotation in Xenopus A6 cells. Biol Sci Space 17: 171-172
10. Moberly JB, Fanestil DD (1988) A monoclonal antibody that recognizes a basolateral membrane protein in A6 epithelial cells. J Cell Physiol 135: 63-70
11. Nikawa T, et al. (2004) Skeletal muscle gene expression in spaceflown rats. FASEB J 18: 522-524
12. Pardo SJ, Patel MJ, Sykes MC, Platt MO, Boyd NL, Sorescu GP, Xu M, van Loon JJ, Wang MD, Jo H (2005) Simulated microgravity using the random positioning machine inhibits differentiation and alters gene expression profiles of 2T3 preosteoblasts. Am J Physiol Cell Physiol 288: C1211-C1221
13. Patel MJ, Liu W, Sykes MC, Ward NE, Risin SA, Risin D, Jo H (2007) Identification of mechanosensitive genes in osteoblasts by comparative microarray studies using the rotating wall vessel and the random positioning machine. J Cell Biochem 101:587-599
14. Sasayama Y (2001) Physiology of vertebrates under micro-gravity with special reference to the Ca metabolism. Biol Sci Space 15: 3-14
15. Sonnenfeld G, Shearer WT (2002) Immune function during space flight. Nutrition 18: 899-903
16. Zayzafoon M, Gathings WE, McDonald JM (2004) Modeled microgravity inhibits osteogenic differentiation of human mesenchymal stem cells and increases adipogenesis. Endocrinology 145: 2421-2432
17. Watenpaugh DE (2001) Fluid volume control during short-term space flight and implications for human performance. J Exp Biol 204:3209-3215