Optical lock-in detection imaging microscopy for contrast-enhanced imaging in living cells

Proceedings of the National Academy of Sciences of the United States of America

Volumn 105, Issue 46, 2008, Pages 17789-17794

  Marriott, Gerard ^a   Mao, Shu ^a   Sakata, Tomoyo ^a   Ran, Jing ^a   Jackson, David K ^a   Petchprayoon, Chutima ^a   Gomez, Timothy J ^a   Warp, Erica ^b   Tulyathan, Orapim ^b   Aaron, Holly L ^b   Isacoff, Ehud Y ^b,c   Yan, Yuling ^d,e  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^d Santa Clara University   (United States)

^e STANFORD UNIVERSITY   (United States)

Author keywords

"ac" imaging; Fluorescence microscopy; High contrast; Optical switches

Indexed keywords

BENZOPYRAN DERIVATIVE; NITROSPIROBENZOPYRAN; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; CELL CULTURE; CONTRAST ENHANCEMENT; CONTROLLED STUDY; FLUORESCENCE; IN VITRO STUDY; IN VIVO STUDY; LARVA; MICROSCOPY; MOUSE; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; WAVEFORM; XENOPUS; ZEBRA FISH;

ACTINS; ANIMALS; CELL SURVIVAL; CELLS, CULTURED; FLUORESCENT DYES; IMAGING, THREE-DIMENSIONAL; MICE; MICROSCOPY, FLUORESCENCE; MICROSCOPY, PHASE-CONTRAST; MUSCLES; NEURONS; NIH 3T3 CELLS; RATS; XENOPUS; ZEBRAFISH;

DANIO RERIO;

EID: 56649099190     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0808882105     Document Type: Article

References (26)

1. Yan Y, Marriott G (2003) Analysis of protein interactions using fluorescence technologies. Curr Opin Chem Biol 7:635-640.
2. Zhang J, Campbell R, Ting A, Tsien R (2002) Creating new fluorescent probes for cell biology. Nat Rev Mol Cell Biol 3:906-918.
3. Westphal M, et al. (1997) Microfilament dynamics in motility and cytokinesis imaged with GFP-actin. Curr Biol 7:176.
4. Axelrod D (2003) Total internal reflection fluorescence microscopy in cell biology. Methods Enzymol 361:1-33.
5. Koyama-Honda I, et al. (2005) Fluorescence imaging for monitoring the colocalization of two single molecules in living cells. Biophys J 88:2126-2136.
6. Hell SW, Dyba M, Jakobs S (2004) Concepts for nanoscale resolution in fluorescence microscopy. Curr Opin Neurobiol 14:599-609.
7. Betzig E, et al. (2006) Imaging intracellular fluorescent proteins at nanometer resolution. Science 313:1642-1645.
8. Rust MJ, Bates M, Zhuang X (2006) Sub-diffraction-limit imaging by stochastic reconstruction optical microscopy (STORM). Nat Methods 3:793-795.
9. Bates M, Huang B, Dempsey GT, Zhuang X (2007) Multicolor super-resolution imaging with photo-switchable fluorescent probes. Science 317:1749-1753.
10. Hofmann M, Eggeling C, Jakobs S, Hell S (2005) Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins. Proc Natl Acad Sci USA 102:565-569.
11. Hess ST, Girirajan TP, Mason MD (2006) Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophys J 91:4258-4272.
12. Aubin J, Histochem J (1979) Autofluorescence of viable cultured mammalian cells. J Histochem Cytochem 27:36-43.
13. So PTC, Kim H, Kochevar IE (1998) Two-photon deep tissue ex vivo imaging of mouse dermal and subcutaneous structures. Opt Express 3:339-350.
14. Eigen M, Maeyer LD, Weissberger A (1963) Technique of Organic Chemistry (Interscience, New York), Vol 8, p 895.
15. MacGregor R, Clegg RM, Jovin TM (1985) Pressure-jump study of the kinetics of ethidium bromide binding to DNA. Biochemistry 24:5503-5510.
16. Mannuzzu L, Moronne M, Isacoff E (1996) Direct physical measure of conformational rearrangement underlying potassium channel gating. Science 271:213-216.
17. Sakata T, Yan Y, Marriott G (2005) Optical switching of dipolar interactions on proteins. Proc Natl Acad Sci USA 102:4759-4764.
18. Sakata T, Yan Y, Marriott G (2005) Family of site-selective molecular optical switches. J Org Chem 70:2009-2013.
19. Ando R, Flors C, Mizuno H, Hofkens J, Miyawaki A (2007) Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Biophys J 92:97-99.
20. Bletz M, Pfeifer-Fukumura U, Kolb U, Baumann W (2002) Ground- and first-excited-singlet-state electric dipole moments of some photochromic spirobenzopyrans in their spiropyran and merocyanine form. J Phys Chem A 106:2232-2236.
21. Görner H, Matter S (2001) Photochromism of nitrospiropyrans: Effects of structure, solvent and temperature. Phys Chem Chem Phys 3:416-423.
22. Giordano L, Jovin T, Irie M, Jares-Erijman E (2002) Diheteroarylethenes as thermally stable photoswitchable acceptors in photochromic fluorescence resonance energy transfer (pcFRET). J Am Chem Soc 124:7481-7489.
23. Dedecker P, et al. (2006) Fast and reversible photoswitching of the fluorescent protein dronpa as evidenced by fluorescence correlation spectroscopy. Biophys J 91:L45-7.
24. Gomez T, Harrigan D, Henely J, Robles E (2003) Working with Xenopus spinal neurons in live cell culture. Methods Cell Biol 71:130-154.
25. Gomez T, Robles E (2003) Imaging calcium dynamics in developing neurons. Methods Enzymol 361:407-422.
26. Scott EK, et al. (2007) Targeting neural circuitry in zebrafish using GAL4 enhancer trapping. Nat Methods 4:323-326.