Frequency, Microbial Interactions, and Antimicrobial Susceptibility of Fusobacterium nucleatum and Fusobacterium necrophorum Isolated from Primary Endodontic Infections

Journal of Endodontics

Volumn 34, Issue 12, 2008, Pages 1451-1456

  Jacinto, Rogério C ^a,b   Montagner, Francisco ^a   Signoretti, Fernanda G C ^a   Almeida, Geovania C ^c   Gomes, Brenda P F A ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

^b FEDERAL UNIVERSITY OF PELOTAS   (Brazil)

^c METHODIST UNIVERSITY OF PIRACICABA   (Brazil)

Author keywords

Anaerobic bacteria; antibiotics; antimicrobial susceptibility; endodontic infection; Fusobacterium necrophorum; Fusobacterium nucleatum

Indexed keywords

AMOXICILLIN; AMOXICILLIN PLUS CLAVULANIC ACID; ANTIINFECTIVE AGENT; CEFACLOR; PENICILLIN G;

ADOLESCENT; ADULT; ANAEROBIC GROWTH; ARTICLE; BIFIDOBACTERIUM; BRAZIL; CHILD; DRUG EFFECT; EUBACTERIUM; FUSOBACTERIUM INFECTION; FUSOBACTERIUM NECROPHORUM; FUSOBACTERIUM NUCLEATUM; HUMAN; ISOLATION AND PURIFICATION; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MIDDLE AGED; PEPTOSTREPTOCOCCUS; PHYSIOLOGY; PORPHYROMONAS GINGIVALIS; PREVOTELLA; STAPHYLOCOCCACEAE; TOOTH FISTULA; TOOTH PERIAPICAL DISEASE; TOOTH PULP; TOOTH PULP DISEASE;

ADOLESCENT; ADULT; AMOXICILLIN; AMOXICILLIN-POTASSIUM CLAVULANATE COMBINATION; ANAEROBIOSIS; ANTI-BACTERIAL AGENTS; BIFIDOBACTERIUM; BRAZIL; CEFACLOR; CHILD; DENTAL FISTULA; DENTAL PULP CAVITY; DENTAL PULP DISEASES; EUBACTERIUM; FUSOBACTERIUM INFECTIONS; FUSOBACTERIUM NECROPHORUM; FUSOBACTERIUM NUCLEATUM; HUMANS; MICROBIAL SENSITIVITY TESTS; MIDDLE AGED; PENICILLIN G; PEPTOSTREPTOCOCCUS; PERIAPICAL ABSCESS; PORPHYROMONAS GINGIVALIS; PREVOTELLA; STAPHYLOCOCCACEAE; YOUNG ADULT;

EID: 56549117298     PISSN: 00992399     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.joen.2008.08.036     Document Type: Article

References (38)

1. Gharbia S.E., Shah H.N., Lawson P.A., and Haapasalo M. The distribution and frequency of Fusobacterium nucleatum subspecies in the human oral cavity. Oral Microbiol Immunol 5 (1990) 324-327
2. Möller A.J. Microbiological examination of root canals and periapical tissues of human teeth. Methodological studies. Odontol Tidskr 74 suppl (1966) 1-380
3. Sundqvist G. Bacteriologic studies of necrotic dental pulps (1976), Odontological Dissertation n°7, University of Umea, Sweden
4. Sundqvist G. Ecology of the root canal flora. J Endod 18 (1992) 427-430
5. Moraes S.R., Siqueira Jr. J.F., Colombo A.P., Rôças I.N., Ferreira M.C.S., and Domingues R.M.C.P. Comparison of the effectiveness of bacterial culture, 16S rDNA directed polymerase chain reaction and checkerboard DNA-DNA hybridization for detection of Fusobacterium nucleatum in endodontic infections. J Endod 28 (2002) 86-89
6. Gomes B.P.F.A., Pinheiro E.T., Gadê-Neto C.R., et al. Microbiological examination of infected dental root canals. Oral Microb Immunol 19 (2004) 71-76
7. Siqueira Jr. J.F., Rôças I.N., Moraes S.R., and Santos K.R.N. Direct amplification of rRNA gene sequences for identification of selected oral pathogens in root canal infections. Int Endod J 35 (2002) 345-351
8. Villanueva L.E.C.P. Fusobacterium nucleatum in endodontic flare-ups. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 93 (2002) 179-183
9. Johnson E.M., Flannagan S.E., and Sedgley C.M. Coaggregation interactions between oral and endodontic Enterococcus faecalis and bacterial species isolated from persistent apical periodontitis. J Endod 32 (2006) 946-950
10. Kolenbrander P.E., Andersen R.N., and Moore L.V. Coaggregation of Fusobacterium nucleatum, Selenomonas flueggei, Selenomonas infelix, Selenomonas noxia, and Selenomonas sputigena with strains from 11 genera of oral bacteria. Infect Immun 57 (1989) 3194-3203
11. Bolstad A.I., Jensen H.B., and Bakken V. Taxonomy, biology, and periodontal aspects of Fusobacterium nucleatum. Clin Microb Rev 9 (1996) 55-71
12. Crippin J.S., and Wang K.K. An unrecognized etiology for pyogenic hepatic abscesses in normal hosts: dental disease. Am J Gastroenterol 12 (1992) 1740-1743
13. González-Gay M.A., Sánches-Andrade A., Cereijo M.J., Pulpeiro J.R., and Armesto V. Pyomiositis and septic arthritis from Fusobacterium nucleatum in non immonocompromised adult. J Reumathol 20 (1993) 518-520
14. Khemaleelakul S., Baumgartner C., and Pruksakorn S. Identification of bacteria in acute endodontic infections and their antimicrobial susceptibility. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 94 (2002) 746-755
15. Jacinto R.C., Gomes B.P.F.A., Ferraz C.C.R., Zaia A.A., and Souza-Filho F.J. Microbiological analysis of infected root canals from symptomatic and asymptomatic teeth with periapical periodontitis and the antimicrobial susceptibility of some isolated anaerobic bacteria. Oral Microb Immunol 18 (2003) 285-292
16. Brazier J.S. Human infections with Fusobacterium necrophorum. Anaerobe 12 (2006) 165-172
17. Garcia M.M., Charlton K.M., and MacKay K.A. Characterization of endotoxin from Fusobacterium necrophorum. Infect Immunol 11 (1975) 371-379
18. Duerden B.I. Virulence factors in anaerobes. Clin Infect Dis 18 suppl 4 (1994) S253-S259
19. Siqueira Jr. J.F., Roças I.N., Alves F.R., and Santos K.R. Selected endodontic pathogens in the apical third of infected root canals: a molecular investigation. J Endod 30 (2004) 638-643
20. Heimdahl A., Von Konow L., Satoh T., and Nord C.E. Clinical appearance of orofacial infections of odontogenic origin in relation to microbiological findings. J Clin Microbiol 22 (1985) 299-302
21. Bolmstrom A. Susceptibility test of anaerobes with E-test. Clin Infect Dis 15 (1992) 483-487
22. Jacinto R.C., Gomes B.P., Shah H.N., Ferraz C.C., Zaia A.A., and Souza-Filho F.J. Incidence and antimicrobial susceptibility of Porphyromonas gingivalis isolated from mixed endodontic infections. Int Endod J 39 (2006) 62-70
23. Syed A.S., and Loesche W.J. Survival of human dental plaque flora in various transport media. Appl Microbiol 24 (1972) 638-644
24. Gomes B.P.F.A., Drucker D.B., and Lilley J.D. Association of specific bacteria with some endodontic signs and symptoms. Int Endod J 27 (1994) 291-298
25. Sundqvist G. Taxonomy, ecology, and pathogenicity of the root canal flora. Oral Surg Oral Med Oral Pathol 78 (1994) 522-530
26. Summanen P., Baron E.J., Citron D.M., Strong C., Wexler H.M., and Finegold S.M. Wadsworth Anaerobic Bacteriology Manual (1993), Star Publishing Company, Belmont, CA
27. Dymoc D., Weightman A.J., Scully C., and Wade W.G. Molecular analysis of microflora associated with dentoalveolar abcesses. J Clin Microbiol 34 (1996) 537-542
28. Sassone L.M., Fidel R.A., Faveri M., et al. A microbiological profile of symptomatic teeth with primary endodontic infections. J Endod 34 (2008) 541-545
29. Chu F.C., Tsang C.S., Chow T.W., and Samaranayake L.P. Identification of cultivable microorganisms from primary endodontic infections with exposed and unexposed pulp space. J Endod 31 (2005) 424-429
30. Gomes B.P.F.A., Drucker D.B., and Lilley J.D. Association of endodontic signs and symptoms with particular combinations of specific bacteria. Int Endod J 29 (1996) 69-75
31. Roças I.N., Siqueira Jr. J.F., Santos K.R., and Coelho A.M. "Red complex" (Bacteroides forsythus, Porphyromonas gingivalis, and Treponema denticola) in endodontic infections: a molecular approach. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 91 (2001) 468-471
32. Lana M.A., Ribeiro-Sobrinho A.P., Stehling R., et al. Microorganisms isolated from root canals presenting necrotic pulp and their drug susceptibility in vitro. Oral Microbiol Immunol 16 (2001) 100-105
33. Khemaleelakul S., Baumgartner J.C., and Pruksakom S. Autoaggregation and coaggregation of bacteria associated with acute endodontic infections. J Endod 32 (2006) 312-318
34. Jansen H.J., van der Hoeven J.S., Walji S., Göertz J.H.C., and Bakkeren J.A.J.M. The importance of immunoglobulin-breakdown supporting the growth of bacteria in oral abscesses. J Clin Periodontol 23 (1996) 717-723
35. Ogawa A.T., Brasil de Souza Tde A., de Uzeda M., Jankevicius J.V., and Jankevicius S.I. Characterization of proteolytic activities of Fusobacterium nucleatum. J Endod 32 (2006) 521-523
36. Hecht D.W. Anaerobes: antibiotic resistance, clinical significance, and the role of susceptibility testing. Anaerobe 12 (2006) 115-121
37. Baumgartner J.C., and Xia T. Antibiotic susceptibility of bacteria associated with endodontic abscesses. J Endod 29 (2003) 44-47
38. Stefanopoulus P.K., and Kolokotronis A.E. The clinical significance of anaerobic bacteria in acute orofacial odontogenic infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 98 (2004) 398-408