Comparison of the effectiveness of bacterial culture, 16S rDNA directed polymerase chain reaction, and checkerboard DNA-DNA hybridization for detection of fusobacterium nucleatum in endodontic infections

Journal of Endodontics

Volumn 28, Issue 2, 2002, Pages 86-89

  Moraes, Saulo Roni ^a   Siqueira Jr , José Freitas ^b   Colombo, Ana Paula ^a   Rôças, Isabela Das Neves ^b   De Souza Ferreira, Maria Candida ^a   Domingues, Regina Maria Cavalcanti Pilotto ^a  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^b Programa de Pós Graduação em Saúde da Família   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

ADULT; ARTICLE; BACTERIAL COUNT; BACTERIAL INFECTION; BACTERIUM IDENTIFICATION; COMPARATIVE STUDY; FUSOBACTERIUM NUCLEATUM; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; TOOTH PERIAPICAL DISEASE; TOOTH PULP DISEASE;

ADULT; BACTERIAL TYPING TECHNIQUES; COLONY COUNT, MICROBIAL; DENTAL PULP NECROSIS; DNA, BACTERIAL; FUSOBACTERIUM INFECTIONS; FUSOBACTERIUM NUCLEATUM; HUMANS; NUCLEIC ACID HYBRIDIZATION; PERIAPICAL PERIODONTITIS; POLYMERASE CHAIN REACTION;

EID: 0036484934     PISSN: 00992399     EISSN: None     Source Type: Journal    
DOI: 10.1097/00004770-200202000-00009     Document Type: Article

References (19)

1. Sundqvist G. Associations between microbial species in dental root canal infections. Oral Microbiol Immunol 1992;7:257-62.
2. Dzink JL, Sheenan MT, Socransky SS. Proposal of three subspecies of Fusobacterium nucleatum Knorr 1922: Fusobacterium nucleatum subsp. nucleatum subsp. nov., comb. nov.; Fusobacterium nucleatum subsp. polymorphum subsp. nov., nom. rev., comb. nov.; and Fusobacterium nucleatum subsp. vincentii subsp. nov., nom. rev., comb. nov. Int J Syst Bacteriol 1990;40:74-8. (Pubitemid 20023671)
3. Gharbia SE, Shah HN. Fusobacterium nucleatum subsp. fusiforme subsp. animalis subsp. nov. as additional subspecies within Fusobacterium nucleatum. Int J Syst Bacteriol 1992;42:296-8.
4. Jousimies-Somer HR, Summanen PH, Finegold SM. Bacteroides, Porphyromonas, Prevotella, Fusobacterium, and other anaerobic Gram-negative bacteria. In: Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, eds. Manual of clinical microbiology. 6th ed. Washington, D.C.: ASM Press, 1995: 603-20.
5. Summanen P, Baron EJ, Citron DM, Strong C, Wexler HM, Finegold SM. Wadsworth anaerobic bacteriology manual. Belmont, CA: Star Publishing Company, 1993.
6. Dix K, Watanabe SM, McArdle S, Lee DI, Randolph C, Moncla B, Schwartz DE. Species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria. J Clin Microbiol 1990;28:319-23. (Pubitemid 20050943)
7. Teng LJ, Hsueh PR, Tsai JC, Chiang FL, Chen CY, Ho SW, Luh KT. PCR assay for species-specific identification of Bacteroides thetaiotamicron. J Clin Microbiol 2000;38:1672-5.
8. Socransky SS, Smith C, Martin L, Paster BJ, Dewhrist FE, Levin AE. "Checkerboard" DNA-DNA hybridization. Biotechniques 1994;17:788-92.
9. Conrads G, Gharbia SE, Gulabivala K, Lampert F, Shah HN. The use of a 16s rDNA directed PCR for the detection of endodontopathogenic bacteria. J Endodon 1997;23:433-8.
10. Siqueira JF Jr, Rôças IN, Favieri A, Santos KRN. Detection of Treponema denticola in endodontic infections by 16S rRNA gene-directed polymerase chain reaction. Oral Microbiol Immunol 2000;15:335-7.
11. Siqueira JF Jr, Rôças IN, Souto R, Uzeda M, Colombo AP. Checkerboard DNA-DNA hybridization analysis of endodontic infections. Oral Surg 2000;89:744-8.
12. Wang RF, Cao WW, Cerniglia CE. PCR detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples. Appl Environ Microbiol 1996;62:1242-7. (Pubitemid 26113201)
13. Rong-Fu W, Beasley JN, Cao WW, Slavik MF, Johnson MG. Development of PCR method specific for Marek's disease virus. Mol Cell Probes 1993;7:127-31. (Pubitemid 23232228)
14. Kreader CA. Design and evaluation of Bacteroides DNA probes for the specific detection of human fecal pollution. Appl Environ Microbiol 1995;61: 1171-9.
15. Moore WEC, Moore LH. Intestinal floras of populations that have a high risk of colon cancer. Appl Environ Microbiol 1995;61:3202-7.
16. Ward DM, Weller R, Bateson MM. 16S rRNA sequences reveal numerous uncultured microorganisms in a natural community. Nature 1990;345: 63-5.
17. Rivieri GR, Weisz KS, Simonson LG, Lukehart SA. Pathogen-related oral spirochetes from dental plaque are invasive. Infect Immun 1991;59:3377-80.
18. Relman DA, Schmidt TM, MacDermott RP, Falkow S. Identification of the uncultured bacillus of Whipple's disease. N Engl J Med 1992;327:293-301.
19. Gharbia SE, Shah HN, Lawson PA, Haapasalo M. The distribution and frequency of Fusobacterium nucleatum subspecies in the human oral cavity. Oral Microbiol Immunol 1990;5:324-7.