An optimized method for histological detection of dopaminergic neurons in Drosophila melanogaster

Journal of Histochemistry and Cytochemistry

Volumn 56, Issue 12, 2008, Pages 1049-1063

  Drobysheva, Daria ^a   Ameel, Kristen ^a   Welch, Brandon ^a   Ellison, Esther ^a   Chaichana, Khan ^a   Hoang, Bryan ^a   Sharma, Shilpy ^a   Neckameyer, Wendi ^b   Srinakevitch, Irina ^c   Murphy, Kelley J ^a   Schmid, Aloisia ^a  

^a UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

^b SAINT LOUIS UNIVERSITY   (United States)

^c AIX MARSEILLE UNIVERSITY   (France)

Author keywords

Dopamine; Drosophila; Fixation; Histology; Parkinson's disease; Technique

Indexed keywords

DOPAMINE; PARAFFIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRAIN MAPPING; BRAIN MATURATION; CELL COUNT; CONTROLLED STUDY; DENDRITIC CELL; DOPAMINERGIC NERVE CELL; DROSOPHILA MELANOGASTER; EMBEDDING; FEMALE; HISTOLOGY; IMMUNOHISTOCHEMISTRY; MALE; NEUROPATHOLOGY; NONHUMAN; PARKINSON DISEASE; PRIORITY JOURNAL; STAINING; TISSUE FIXATION;

ANIMALS; BRAIN; CELL COUNT; DENDRITES; DOPAMINE; DROSOPHILA MELANOGASTER; FIXATIVES; HISTOCYTOLOGICAL PREPARATION TECHNIQUES; IMMUNOHISTOCHEMISTRY; NEURONS; STAINING AND LABELING; TISSUE EMBEDDING; TISSUE FIXATION;

DROSOPHILA MELANOGASTER; HEXAPODA; PARKINSONIA;

EID: 56449121807     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/jhc.2008.951137     Document Type: Article

References (27)

1. Anathbandhu C, Bowling K, Funderburk C, Lawal H, Inamdar A, Wang Z, O'Donnell JM (2007) Interaction of genetic and environmental factors in a Drosophila Parkinsonism model. J Neurosci 27:2457-2467
2. Ascherio A, Chen H, Weisskopf MG, O'Reilly E, McCullough ML, Calle EE, Schwarzschild MA, et al. (2006) Pesticide exposure and risk for Parkinson's disease. Ann Neurol 60:197-203
3. Auluck PK, Chan HYE, Trojanowski JQ, Lee VM-Y, Bonini NM (2002) Chaperone suppression of α-synuclein toxicity in a Drosophila model for Parkinson's disease. Science 295:865-868
4. Chapman RF (2004) The nervous system. In The Insects: Structure and Function. Cambridge, Cambridge University Press, 561-569
5. Cooper PW (1996) Explosives Engineering. Berlin, Wiley-VHC
6. Davis RJ, Tavsanli BC, Dittrich C, Walldorf U, Mardon G (2003) Drosophila retinal homeobox (drx) is not required for establishment of the visual system but is required for brain and clypeus development. Dev Biol 259:272-287
7. DeGiusti DL, Ezman L (1955) Two methods for serial sectioning of arthropods and insects. Trans Am Microsc Soc 74:197-201
8. Feany MB, Bender WW (2000) A Drosophila model of Parkinson's disease. Nature 404:394-398
9. Giaccone G, Canciani B, Puoti G, Goffredo D, Iussich S, Fociani P, Tagliavini F, et al. (2000) Creutzfeldt-Jakob disease: Carnoy's fixative improves the immunohistochemistry of the proteinasse K-resistant prion protein. Brain Pathol 10:326-329
10. Haider SG, Servos G, Tran N (2007) Structural and histological analysis of Leydig cell steroidogenic function. In Payne AH, Hardy MP, eds. The Leydig Cell in Health and Disease. Totowa, NJ, Humana Press, 33-46
11. Homberg U, Davis NT, Hildebrane JG (1991) Peptide-immunocytochemistry of neurosecretory cells in the brain and retrocerebral complex of the sphinx moth Manduca sexta. J Comp Neurol 303:35-52
12. Kiernan AJ (1981) Fixation. In Histological and Histochemical Methods: Theory & Practice. Oxford, Pergamon Press, 8-24
13. Klatt EC (1994) Internet Pathology Laboratory on WebPath. http://library.med.utah.edu/WebPath/webpath.html (accessed February 13, 2008)
14. Mitchison TJ, Sedat J (1983) Localization of antigenic determinants in whole Drosophila embryos. Dev Biol 99:261-264
15. Nassel DR, Elekes K (1992) Aminergic neurons in the brain of blowflies and Drosophila: dopamine- and tyrosine hydroxylase-immunoreactive neurons and their relationship with putative histaminergic neurons. Cell Tissue Res 267:147-167
16. Neckameyer W, Woodrome S, Holt B, Mayer A (2000) Dopamine and senescence in Drosophila melanogaster. Neurobiol Aging 21:145-152
17. Nussbaum RL, Christopher EE (2003) Alzheimer's disease and Parkinson's disease. N Engl J Med 348:1356-1364
18. Pendleton RG, Parvez F, Saved M, Hillman R (2002) Effects of pharmacological agents upon a transgenic model of Parkinson's disease in Drosophila melanogaster. J Pharmacol Exp Ther 300:91-96
19. Pesah Y, Burgess H, Middlebrooks B, Ronningen K, Prosser J, Tirunagaru V, Zysk J, et al. (2005) Whole-mount analysis reveals normal numbers of dopaminergic neurons following mis-expression of α-synuclein in Drosophila. Genesis 41:154-159
20. Richards AG (1952) Studies on arthropod cuticle. VII: patent and masked carbohydrate in the epicuticle of insects. Science 115:206-208
21. Sabatini DD (2005) In awe of subcellular complexity: 50 years of trespassing boundaries within the cell. Annu Rev Cell Dev Biol 21:1-33
22. Seroogy K, Tsuruo Y, Walsh J, Fahrenkrug J, Enison PC, Goldstein M (1988) Further analysis of presence of peptides in dopamine neurons. Cholecystokini, peptide histodine-isoleucine/vasoactive intestinal polypeptide and substance P in rat upramammillary region and mesencephalon. Exp Brain Res 72:523-534
23. St. Johnston D (2002) The art and design of generic screen: Drosophila melanogaster. Nat Rev Genet 3:176-188
24. Wang D, Qian L, Xiong H, Liu J, Neckameyer WS, Oldham S, Xia K, et al. (2006) Antioxidants protect PINK1-dependent dopaminergic neurons in Drosophila. Proc Natl Acad Sci USA 103:13520-13525
25. Weinstock M, McDonald FJD (1969) The use of mushroom enzymes for softening insect cuticle. Aust J Entomol 8:115-116
26. Whitworth AJ, Theodore DA, Greene JC, Benes H, Wes PD, Pallanck LJ (2005) Increased glutathione S-transferase activity rescues dopaminergic neuron loss in a Drosophila model of Parkinson's disease. Proc Natl Acad Sci USA 102:8024-8029
27. Yang Y, Nishimura I, Takahashi R, Lu B (2003) Parkin suppresses dopaminergic neuro-selective neurotoxicity induced by Pael-R in Drosophila. Neuron 37:911-924