Biochemical purification and functional analysis of complexes between the G-protein subunit Gβ5 and RGS proteins

Methods in Enzymology

Volumn 390, Issue , 2004, Pages 149-162

  Witherow, D Scott ^a   Slepak, Vladlen Z ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN; PROTEIN DERIVATIVE; PROTEIN SUBUNIT;

ANTIBODY AFFINITY; ARTICLE; BIOCHEMISTRY; COMPLEX FORMATION; CORRELATION ANALYSIS; DIMERIZATION; ENZYME ASSAY; ENZYME PURIFICATION; ENZYME REGULATION; ENZYME STABILITY; FLUORESCENCE RESONANCE ENERGY TRANSFER; FRACTIONATION; IMAGING SYSTEM; IMMUNOPRECIPITATION; MOLECULAR BIOLOGY; MOLECULAR MECHANICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN STABILITY; RETINA; SIGNAL TRANSDUCTION;

EID: 5644272616     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(04)90010-9     Document Type: Chapter

References (38)

1. Arshavsky V., Bownds M.D. Regulation of deactivation of photoreceptor G protein by its target enzyme and cGMP. Nature. 357:1992;416-417
2. Berman D.M., Gilman A.G. Mammalian RGS proteins: Barbarians at the gate. J. Biol. Chem. 273:1998;1269-1272
3. Berman D.M., Kozasa T., Gilman A.G. The GTPase-activating protein RGS4 stabilizes the transition state for nucleotide hydrolysis. J. Biol. Chem. 271:1996a;27209-27212
4. Berman D.M., Wilkie T.M., Gilman A.G. GAIP and RGS4 are GTPase-activating proteins for the Gi subfamily of G protein alpha subunits. Cell. 86:1996b;445-452
5. Berstein G., Blank J.L., Jhon D.Y., Exton J.H., Rhee S.G., Ross E.M. Phospholipase C-beta 1 is a GTPase-activating protein for Gq11, its physiologic regulator. Cell. 70:1992;411-418
6. Bigay J., Chabre M. Purification of transducin. Methods Enzymol. 237:1994;139-146
7. Cabrera J.L., de Freitas F., Satpaev D.K., Slepak V.Z. Identification of the Gbeta5-RGS7 protein complex in the retina. Biochem. Biophys. Res. Commun. 249:1998;898-902
8. Chidiac P., Ross E.M. Phospholipase C-beta1 directly accelerates GTP hydrolysis by Galphaq and acceleration is inhibited by Gbeta gamma subunits. J. Biol. Chem. 274:1999;19639-19643
9. De Vries L., Mousli M., Wurmser A., Farquhar M.G. GAIP, a protein that specifically interacts with the trimeric G protein G alpha i3, is a member of a protein family with a highly conserved core domain. Proc. Natl. Acad. Sci. USA. 92:1995;11916-11920
10. Gold S.J., Ni Y.G., Dohlman H.G., Nestler E.J. Regulators of G-protein signaling (RGS) proteins: Region-specific expression of nine subtypes in rat brain. J. Neurosci. 17:1997;8024-8037
11. Hepler J.R. RGS protein and G protein interactions: A little help from their friends. Mol. Pharmacol. 64:2003;547-549
12. Higgins J.B., Casey P.J. The role of prenylation in G-protein assembly and function. Cell Signal. 8:1996;433-437
13. 4. J. Biol. Chem. 276:2001;4227-4235
14. Jones M.B., Garrison J.C. Instability of the G-protein beta5 subunit in detergent. Anal. Biochem. 268:1999;126-133
15. Levay K., Cabrera J.L., Satpaev D.K., Slepak V.Z. Gbeta5 prevents the RGS7-Galphao interaction through binding to a distinct Ggamma-like domain found in RGS7 and other RGS proteins. Proc. Natl. Acad. Sci. USA. 96:1999;2503-2507
16. Makino E.R., Handy J.W., Li T., Arshavsky V.Y. The GTPase activating factor for transducin in rod photoreceptors is the complex between RGS9 and type 5 G protein beta subunit. Proc. Natl. Acad. Sci. USA. 96:1999;1947-1952
17. Miyawaki A., Tsien R.Y. Monitoring protein conformations and interactions by fluorescence resonance energy transfer between mutants of green fluorescent protein. Methods Enzymol. 327:2000;472-500
18. Muntz K.H., Sternweis P.C., Gilman A.G., Mumby S.M. Influence of gamma subunit prenylation on association of guanine nucleotide-binding regulatory proteins with membranes. Mol. Biol. Cell. 3:1992;49-61
19. Posner B.A., Gilman A.G., Harris B.A. Regulators of G protein signaling 6 and 7: Purification of complexes with Gbeta5 and assessment of their effects on G protein-mediated signaling pathways. J. Biol. Chem. 274:1999;31087-31093
20. Rojkova A.M., Woodard G.E., Huang T.C., Combs C.A., Zhang J.H., Simonds W.F. Ggamma subunit-selective G protein beta 5 mutant defines regulators of G protein signaling protein binding requirement for nuclear localization. J. Biol. Chem. 278:2003;12507-12512
21. Rose J.J., Taylor J.B., Shi J., Cockett M.I., Jones P.G., Hepler J.R. RGS7 is palmitoylated and exists as biochemically distinct forms. J. Neurochem. 75:2000;2103-2112
22. Scholich K., Mullenix J.B., Wittpoth C., Poppleton H.M., Pierre S.C., Lindorfer M.A., Garrison J.C., Patel T.B. Facilitation of signal onset and termination by adenylyl cyclase. Science. 283:1999;1328-1331
23. Sibley D.R., Lefkowitz R.J. Molecular mechanisms of receptor desensitization using the beta-adrenergic receptor-coupled adenylate cyclase system as a model. Nature. 317:1985;124-129
24. Siderovski D.P., Strockbine B., Behe C.I. Whither goest the RGS proteins? Crit. Rev. Biochem. Mol. Biol. 34:1999;215-251
25. Siegel R.M., Chan F.K., Zacharias D.A., Swofford R., Holmes K.L., Tsien R.Y., Lenardo M.J. Measurement of molecular interactions in living cells by fluorescence resonance energy transfer between variants of the green fluorescent protein. Sci. STKE. 2000:2000;PL1
26. Simonds W.F., Butrynski J.E., Gautam N., Unson C.G., Spiegel A.M. G-protein beta gamma dimers: Membrane targeting requires subunit coexpression and intact gamma C-A-A-X domain. J. Biol. Chem. 266:1991;5363-5366
27. Snow B.E., Betts L., Mangion J., Sondek J., Siderovski D.P. Fidelity of G protein beta-subunit association by the G protein gamma-subunit-like domains of RGS6, RGS7, and RGS11. Proc. Natl. Acad. Sci. USA. 96:1999;6489-6494
28. Snow B.E., Krumins A.M., Brothers G.M., Lee S.F., Wall M.A., Chung S., Mangion J., Arya S., Gilman A.G., Siderovski D.P. A G protein gamma subunit-like domain shared between RGS11 and other RGS proteins specifies binding to Gbeta5 subunits. Proc. Natl. Acad. Sci. USA. 95:1998;13307-13312
29. Sondek J., Siderovski D.P. Ggamma-like (GGL) domains: New frontiers in G-protein signaling and beta-propeller scaffolding. Biochem. Pharmacol. 61:2001;1329-1337
30. Tesmer J.J., Berman D.M., Gilman A.G., Sprang S.R. Structure of RGS4 bound to AlF4-activated G(i alpha1): Stabilization of the transition state for GTP hydrolysis. Cell. 89:1997;251-261
31. Watson A.J., Aragay A.M., Slepak V.Z., Simon M.I. A novel form of the G protein beta subunit Gbeta5 is specifically expressed in the vertebrate retina. J. Biol. Chem. 271:1996;28154-28160
32. Watson A.J., Katz A., Simon M.I. A fifth member of the mammalian G-protein beta-subunit family: Expression in brain and activation of the beta 2 isotype of phospholipase C. J. Biol. Chem. 269:1994;22150-22156
33. Witherow D.S., Slepak V.Z. A novel kind of G protein heterodimer: The Gbeta5-RGS complex. Receptors Channels. 9:2003;205-212
34. Witherow D.S., Tovey S.C., Wang Q., Willars G.B., Slepak V.Z. G beta 5.RGS7 inhibits G alpha q-mediated signaling via a direct protein-protein interaction. J. Biol. Chem. 278:2003;21307-21313
35. Witherow D.S., Wang Q., Levay K., Cabrera J.L., Chen J., Willars G.B., Slepak V.Z. Complexes of the G protein subunit Gbeta 5 with the regulators of G protein signaling RGS7 and RGS9: Characterization in native tissues and in transfected cells. J. Biol. Chem. 275:2000;24872-24880
36. Zacharias D.A., Violin J.D., Newton A.C., Tsien R.Y. Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science. 296:2002;913-914
37. Zhang J.H., Barr V.A., Mo Y., Rojkova A.M., Liu S., Simonds W.F. Nuclear localization of G protein beta 5 and regulator of G protein signaling 7 in neurons and brain. J. Biol. Chem. 276:2001;10284-10289
38. Zhang J.H., Lai Z., Simonds W.F. Differential expression of the G protein beta(5) gene: Analysis of mouse brain, peripheral tissues, and cultured cell lines. J. Neurochem. 75:2000;393-403